Staphylococcus aureus is a major cause of nosocomial infections and secretes diverse spectrum virulence determinants as well forms biofilm. The emergence antibiotic-resistant S. highlights the need for alternative therapeutics other than conventional antibiotics. One route to meet this screening small molecule derivatives potential anti-infective activity. Using previously optimized C. elegans - screen, we identified benzimidazole derivative, UM-C162, which rescued nematodes from infection....

Abstract Background The limited antibiotic options for effective control of methicillin-resistant Staphylococcus aureus infections has led to calls new therapeutic approaches combat this human pathogen. An alternative approach MRSA is through the use anti-infective agents that selectively disrupt virulence-mediated pathways without affecting microbial cell viability or by modulating host natural immune defenses Methods We established a C. elegans – S. liquid-based assay screen potential...

Hydrogels comprised of alginate and gelatin are potential biomaterials for extrusion-based bioprinting. However, existing polymeric inks inadequate in printability, shape fidelity mechanical properties that desire to produce scaffold using 3D Here, a novel printable hydrogel-based ink solution gelatin-alginate (GA) reinforced by boron nitride nanotubes (BNNTs) was developed bioprinting tissue engineering applications. The solutions were tested printing accuracy, compressive behaviour, while...

Chronic bacterial infections occur as a result of the infecting pathogen's ability to live within biofilm, hence escaping detrimental effects antibiotics and immune defense system. Burkholderia pseudomallei, gram-negative facultative pathogen, is distinctive in its survive phagocytic non-phagocytic cells, persist vivo for many years subsequently leading relapse well development chronic disease. The capacity has been attributed form biofilm. However, underlying biology B. pseudomallei biofilm...

Zinc oxide nanoparticles (ZnONPs) are employed widely in various fields. However, these ZnONPs may potentially inhibit enzyme activities. Cytochrome P450 (P450-) isozymes, which play a crucial function human bio-transforming drugs, can be inhibited by ZnONPs, leading to slower clearance of co-administrated drugs and elevated drug plasma concentrations. This study aimed reveal the inhibitory effects ZnO nano-sized particles, (<50 nm ZnONP50), (<100nm ZnONP100), bulk particles (ZnOBP) on...

Significance Bacterial pathogens use multiple mechanisms to survive and proliferate within an infected host, including blunting the host’s ability defend itself from pathogenic assaults. We identified a new immune suppression mechanism by Burkholderia pseudomallei , causative agent of melioidosis, which life-threatening disease in humans. Analyses whole-genome transcriptional responses Caenorhabditis elegans B. infection revealed that through its type III secretion system, recruits host...

Alginate (Alg) hydrogels are commonly used as bioinks in 3D bioprinting. However, one of the significant drawbacks using Alg is their unstable mechanical properties. In this study, a novel hydrogel-based ink composed reinforced with functionalised boron nitride nanotubes (f-BNNTs) was developed and systematic quantitative characterisation conducted to validate its printability, physiochemical properties biocompatibility. The contact angle test results indicated good structural stability...

Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, identification alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans-S. screening platform to identify potential anti-infective agents from collection natural extracts and synthetic compounds. One hits obtained screen was aqueous extract Orthosiphon stamineus leaves (UE-12) that enhanced survival infected nematodes without interfering with...

Over the last decade, there has been an increasing interest in use of bioceramics for biomedical purposes. Bioceramics, specifically those made calcium phosphate, are commonly used dental and orthopaedic applications. In this context, hydroxyapatite (HA) is considered a viable option hard tissue engineering applications given its compositional similarity to bioapatite. However, owing their poor mechanobiology biodegradability, traditional HA-based composites have limited utilisation...

Abstract Toxins are believed to play a crucial role in Burkholderia pseudomallei pathogenicity, however date, only few have been identified. The discovery of additional toxic molecules is limited by the lack sensitive indicator B. toxicity. Previously, from whole genome transcriptome analysis -infected Caenorhabditis elegans , we noted significant overexpression number worm genes encoding detoxification enzymes, indicating host’s attempt clear bacterial molecules. One these genes, ugt–29...

Burkholderia pseudomallei , a Gram-negative pathogen, is the causative agent of melioidosis in humans. This bacterium can be isolated from soil, stagnant and salt-water bodies, human animal clinical specimens. While extensive studies have contributed to our understanding B. pathogenesis, little known about how harmless soil adapts when it shifts host exhibits its virulence. The bacterium’s large genome encodes an array factors that support pathogen’s ability survive under stressful...

This study investigated the vasorelaxant effects of schwarzinicine A, an alkaloid recently reported from Ficus schwarzii Koord. Regulation calcium homeostasis in vascular smooth muscle cells (VSMC) is viewed as one main mechanisms for controlling blood pressure. L-type voltage-gated channel (VGCC) blockers are commonly used hypertension. Recently, transient receptor potential canonical (TRPC) channels were found vessels different animal species with evidence their roles regulation...

Graphene has gained tremendous attention due to its unlimited potential in various applications while poly(lactic acid) (PLA) is a biodegradable thermoplastic polyester produced from fermenting corn starch. The incorporation of graphene into PLA been proven exhibit excellent mechanical and thermal properties. However, there are not many reports on the toxic effect these materials towards living organisms. In this study, we investigated possible toxicity PLA-graphene live animal model,...

The significant influence of khat, cathinone, and cathine on appetite suppression, reproduction impairment, inhibitory effects CYPs enzyme activity is widely recognised, but much remains unknown about its mode action. This study demonstrated that exposure to high doses KEE, sibutramine at 12.5, 1.8, 1.8 mg/ml showed the most visible reduction in pharyngeal pumping rate, respectively. C. elegans an anomalous dynamic rate upon these compounds overall, a reduced amount progeny. extended...

N-Phenethyl-1-phenyl-pentan-3-amine (1) is a new compound synthesised as simplified analogue of schwarzinicine A (2), natural extracted from Ficus schwarzii. Compound 1 differs 2 due to its structural simplification, featuring two phenyl rings without methoxy substitution, opposed 2, which possesses three 3,4-dimethoxy aromatic rings. Our previous research findings highlighted the calcium-inhibitory effects but mechanism action for remains unexplored, serving primary focus this study....

This work aimed to investigate the effects of boron nitride nanotubes (BNNTs) on mechanical and thermal properties BNNT-polycaprolactone (PCL) nanocomposites. BNNTs were synthesized via co-precipitation annealing process by using amorphous powder as a precursor. PCL-BNNT nanocomposites prepared sonication curing. Their morphology, tensile characterized. Morphological observation revealed that arbitrarily evenly dispersed within PCL which indicated good dispersion in polymer matrix. Both...

Thallium-based high temperature superconductor (HTS) with nominal starting composition (Tl 0.85 Cr 0.15 )Sr 2 CaCu O 7 -δ (Tl-1212) was prepared using purity oxide powders via solid state reaction method. Small amounts (0.0 – wt. %) of cobalt ferrite nanoparticles (CoFe 4 ) were added into Tl-1212 superconductors. The effect CoFe addition on the critical ( T c ), phase formation and microstructure properties including elemental compositional analysis studied. samples investigated by...