A5020835389- Ion Channels and Receptors
- Crystallization and Solubility Studies
- Cytomegalovirus and herpesvirus research
- X-ray Diffraction in Crystallography
- Genetic Neurodegenerative Diseases
- Boron Compounds in Chemistry
- Phytochemicals and Antioxidant Activities
- Pneumonia and Respiratory Infections
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Herpesvirus Infections and Treatments
- Ion channel regulation and function
- Polyomavirus and related diseases
- Crystallography and molecular interactions
- Boron and Carbon Nanomaterials Research
- Essential Oils and Antimicrobial Activity
- Lysosomal Storage Disorders Research
- Phytochemistry and biological activities of Ficus species
- Carbohydrate Chemistry and Synthesis
- Hemoglobin structure and function
- Microbial infections and disease research
- Phytochemistry and Biological Activities
- Viral-associated cancers and disorders
- Peptidase Inhibition and Analysis
- Neuroendocrine Tumor Research Advances
University of Leeds
2009-2024
Angelina College
2022
Centogene (Germany)
2019
University of Reading
2017-2018
University Hospital Heidelberg
2015
Heidelberg University
2005-2015
German Center for Lung Research
2015
University of Tübingen
2006-2013
Medical University of Vienna
2006-2011
TH Bingen University of Applied Sciences
2008
Abstract Two prominent concepts for the sensing of shear stress by endothelium are PIEZO1 channel as a mediator mechanically activated calcium ion entry and PECAM1 cell adhesion molecule apex triad with CDH5 VGFR2. Here, we investigated if there is relationship. By inserting non-disruptive tag in native mice, reveal situ overlap PECAM1. Through reconstitution high resolution microscopy studies show that interacts directs it to cell-cell junctions. extracellular N-terminus critical this, but...
Conversion of soluble α-synuclein into insoluble and fibrillar inclusions is a hallmark Parkinson's disease other synucleinopathies. Accumulating evidence points towards relationship between its generation at nerve terminals structural synaptic pathology. Little known about the pathogenic impact conversion deposition nigrostriatal dopaminergic synapses in transgenic mice, mainly owing to expression limitations construct. Here, we explore whether both rat as model bacterial artificial...
There are many efficient ways to connect proteins at termini. However, connecting a loop is difficult because of lower flexibility and variable environment. Here, we have developed DogCatcher, protein that forms spontaneous isopeptide bond with DogTag peptide. DogTag/DogCatcher was generated initially by splitting Streptococcus pneumoniae adhesin. We optimized through rational design evolution, increasing reaction rate 250-fold establishing millimolar solubility DogCatcher. When fused...
Background. Cytomegalovirus (CMV) is a major pathogen in lung transplant recipients (LTRs). The emergence of different CMV strains and blood after transplantation has not yet been analyzed. Methods. In total, 75 serum 91 broncheoalveolar lavage (BAL) samples obtained from 25 LTRs the follow-up were tested for presence strains. gB, gN, gO genes isolates analyzed by subtype-specific PCR, restriction fragment length polymorphism (RFLP), sequencing, phylogenetic analysis. Results. Mixed...
Cytomegalovirus (CMV) is a significant cause of morbidity and mortality in lung transplant recipients (LTRs). The aim the present study was to elucidate relationship between CMV DNA load compartment that plasma. For determination, level plasma bronchoalveolar lavage (BAL) samples measured total 97 paired BAL obtained from 25 LTRs. original virus concentration epithelial lining fluid (ELF) calculated by correcting for dilution using urea method. In addition, Epstein-Barr (EBV) human...
<h3>Background</h3> To guide time- and cost-efficient analyses of the increasing number autosomal-dominant spinocerebellar ataxia genes (SCAs), more information about frequency distributions, phenotypic characteristics optimal diagnostic strategies is warranted. <h3>Objective</h3> assess prevalence spectrum SCA15 to confirm multiplex ligation-dependent probe amplification (MLPA) as a robust efficient strategy for routine molecular diagnosis. <h3>Methods</h3> Fifty-six German SCA families...
Objectives To describe changes over time in extent of idiopathic pulmonary fibrosis (IPF) at multidetector computed tomography (MDCT) assessed by semi-quantitative visual scores (VSs) and fully automatic histogram-based quantitative evaluation to test the relationship between these two methods quantification. Methods Forty IPF patients (median age: 70 y, interquartile: 62-75 years; M:F, 33:7) that underwent 2 MDCT different points with a median interval 13 months (interquartile: 10-17...
The putative cache (Ca 2+ channel and chemotaxis receptor) domain containing 1 (CACHD1) protein has predicted structural similarities to members of the α2δ voltage-gated Ca auxiliary subunit family. CACHD1 mRNA were highly expressed in male mammalian CNS, particular thalamus, hippocampus, cerebellum, with a broadly similar tissue distribution V 3 subunits, 3.1. In expression studies, increased cell-surface localization 3.1, these proteins close proximity at cell surface, consistent formation...
Background and Purpose The TRPC1, TRPC4, TRPC5 proteins form homotetrameric or heterotetrameric, calcium‐permeable cation channels that are involved in various disease states. Recent research has yielded specific potent xanthine‐based TRPC1/4/5 inhibitors. Here, we investigated the possibility of activators these channels. Experimental Approach An analogue inhibitor Pico145, AM237, was synthesized its activity using HEK cells overexpressing TRPC5, TRPC4–C1, TRPC5–C1, TRPC1:C4 TRPC1:C5...
Spinocerebellar ataxia 17 (SCA17) is an autosomal-dominant, late-onset neurodegenerative disorder caused by expanded polyglutamine (polyQ) repeat in the TATA-box-binding protein (TBP). To further investigate this devastating disease, we sought to create a first transgenic rat model for SCA17 that carries full human cDNA fragment of TBP gene with 64 CAA/CAG repeats (TBPQ64). In line previous observations mouse models SCA17, TBPQ64 rats show severe neurological phenotype including ataxia,...
Niemann-Pick type C disease (NP-C) is a rare, autosomal recessive lipid storage disorder. At least 96% of all NP-C patients link to NPC1 which encodes for lysosomally-targeted protein. We describe the complete genomic sequence 57,052 kb corresponding transcribed region human including several exonic and intronic single nucleotide polymorphisms (SNPs). Sequencing exons, splice sites, promoter in 12 unrelated Caucasian revealed nine novel four known most likely disease-causing mutations. Ten...
Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. During acute, lytic phase infection, DNA can also be detected in serum. In the present study, diagnostic utility EBV detection and quantitation serum was investigated. The level 98 immunocompetent patients aged 1-47 years with symptomatic, antibody-confirmed assessed using a quantitative real-time PCR assay. association between load time after onset disease, age...
Human cytomegalovirus (CMV) is a major cause of disease and transplant dysfunction in lung recipients. Simultaneous emergence more than one CMV-genotype can occur, appears to be disadvantageous for the patient. In this study, dynamics individual CMV-genotypes blood was assessed within mixed populations emerging after transplantation. 69 plasma 76 bronchoalveolar lavage samples 16 recipients with infections first year posttransplantation each glycoprotein B (gB) H (gH) genotypes selectively...
Nonsense mutations, which occur in ∼11% of patients with genetic disorders, introduce premature termination codons (PTCs) that lead to truncated proteins and promote nonsense-mediated mRNA decay. Aminoglycosides such as G418 permit PTC readthrough so may be used address this problem. However, their effects are variable between patients, making clinical use aminoglycosides challenging. In study, we tested whether TRPC nonselective cation channels contribute the effect by controlling cellular...
Sublethal carbon monoxide poisoning causes prolonged neurological damage involving oxidative stress. Given the central role of Ca(2+) homeostasis and its vulnerability to stress, we investigated whether CO disrupts neuronal homeostasis.Cytosolic transients evoked by muscarine in SH-SY5Y cells were (applied via donor CORM-2), capacitative entry (CCE) was dramatically enhanced. store mobilization cyclopiazonic acid similarly augmented, as subsequent CCE, that thapsigargin. rises depolarization...
Diazirine-containing photoaffinity probes, based on the potent and selective TRPC1/4/5 channel inhibitor Pico145, allowed development of an assay to probe cellular interactions between TRPC5 protein xanthine-based modulators.
ABSTRACT Directly probing the endogenous biological roles of target proteins with high spatial and temporal resolution, as non-invasively reproducibly possible, is a shared conceptual goal for research across many fields, well targeted therapies. Here we describe rational design test-case practical implementation photopharmacological paradigm to empower high-performance photomodulation studies in vivo . TRPC4/5 ion channels are involved spatiotemporally resolved circuits, from pain anxiety,...