A5060639994- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Neurobiology and Insect Physiology Research
- bioluminescence and chemiluminescence research
- Molecular Sensors and Ion Detection
- Biochemical Analysis and Sensing Techniques
- Electrochemical sensors and biosensors
- Retinal Development and Disorders
- Neonatal Health and Biochemistry
- Olfactory and Sensory Function Studies
- Sulfur Compounds in Biology
- Selenium in Biological Systems
- Neuroscience and Neural Engineering
- Photodynamic Therapy Research Studies
- Advanced biosensing and bioanalysis techniques
- Molecular Communication and Nanonetworks
- Advanced Fluorescence Microscopy Techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Cell Image Analysis Techniques
- Liver Disease and Transplantation
- Mitochondrial Function and Pathology
- Electrochemical Analysis and Applications
- Ion Channels and Receptors
Saarland University
2021-2023
Friedrich Schiller University Jena
2014-2019
Jena University Hospital
2014-2019
Photoswitchable reagents can be powerful tools for high-precision biological control. TRPC5 is a Ca
Biliverdin and bilirubin were previously considered end products of heme catabolism; now, however, there is evidence for further degradation to diverse bioactive products. Z-BOX A B arise upon oxidation with unknown implications hepatocellular function integrity. We studied the impact on hepatic functions explored their alterations in health cholestatic conditions.Functional mechanisms investigated rats, hepatocytic HepG2 HepaRG cells, human immortalized hepatocytes, isolated perfused...
Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, OptoDArG—for ultrarapid activation deactivation native TRPC2 channels in mouse vomeronasal sensory neurons olfactory type B cells, as well heterologously expressed human TRPC6 Photoconversion can be...
ABSTRACT Directly probing the endogenous biological roles of target proteins with high spatial and temporal resolution, as non-invasively reproducibly possible, is a shared conceptual goal for research across many fields, well targeted therapies. Here we describe rational design test-case practical implementation photopharmacological paradigm to empower high-performance photomodulation studies in vivo . TRPC4/5 ion channels are involved spatiotemporally resolved circuits, from pain anxiety,...
Abstract Photonic experiments are of key importance in life sciences but light-induced side effects serious confounding factors. Here we introduce roNa V 2, an engineered voltage-gated Na + channel harboring a selenocysteine its inactivation motif, as non-photonic, sensitive, gateable, and reversible sensor for membrane-delimited reactive species. 2 allows the assessment chemical modification induced fluorescence microscopy settings with high sensitivity time resolution it demonstrates...
Photoswitchable reagents to modulate protein activity are powerful tools for high-spatiotemporal-precision control over endogenous biological functions. TRPC5 is a Ca2+-permeable cation channel with distinct tissue-specific roles, ranging from synaptic function hormone regulation. Achieving spatially-resolved in particular cells or tissues, and temporal regulation targeted cells, therefore crucial milestones towards understanding harnessing the biology of TRPC5. Here we develop first...
Abstract Photoswitchable reagents can be powerful tools for high‐precision biological control. TRPC5 is a Ca 2+ ‐permeable cation channel with distinct tissue‐specific roles, from synaptic function to hormone regulation. Reagents giving spatiotemporally‐resolved control over activity may key understanding and harnessing its biology. Here we develop the first photoswitchable TRPC5‐modulator, BTDAzo , address this goal. photocontrol currents in cell culture, as well controlling endogenous...