A5100665061- Cancer, Hypoxia, and Metabolism
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Probiotics and Fermented Foods
- Atherosclerosis and Cardiovascular Diseases
- Angiogenesis and VEGF in Cancer
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Inflammasome and immune disorders
- Cell death mechanisms and regulation
- Cytokine Signaling Pathways and Interactions
- Corneal surgery and disorders
National Heart Lung and Blood Institute
2013-2024
Columbia University Irving Medical Center
2018-2024
National Institute of Metrology
2009-2024
National Institutes of Health
2013-2024
Institute of Zoology
2022-2024
Chinese Academy of Sciences
2022-2024
Columbia University
2021-2024
Xinjiang University
2024
Ludong University
2020-2024
Chongqing Medical University
2024
The mechanisms driving atherothrombotic risk in individuals with JAK2 V617F ( Jak2 VF) positive clonal hematopoiesis or myeloproliferative neoplasms are poorly understood.The goal of this study was to assess atherosclerosis and underlying hypercholesterolemic mice hematopoietic VF expression.Irradiated low-density lipoprotein receptor knockout Ldlr-/-) were transplanted bone marrow from wild-type fed a high-fat high-cholesterol Western diet. Hematopoietic functions characterized. After 7...
Up to 90% of cancer-related deaths are caused by metastatic cancer. Circulating tumor cells (CTCs), a type cancer cell that spreads through the blood after detaching from solid tumor, essential for establishment distant metastasis given As new liquid biopsy, analysis CTCs offers possibility avoid invasive tissue biopsy procedures with practical implications diagnostics. The fundamental challenges analyzing and profiling extremely low abundances in intrinsic heterogeneity CTCs. Various...
Olfactomedin 4 (OLFM4) is a glycoprotein that has been found to be up-regulated in inflammatory bowel diseases and Helicobacter pylori infected patients. However, its role biological processes such as inflammation or other immune response not known. In this study, we generated OLFM4 KO mice investigate potential role(s) of gastric mucosal responses H. infection. colonization the mucosa was significantly lower compared with WT littermates. The reduced bacterial load associated enhanced...
The RAV (related to ABI3/viviparous 1) group of transcription factors (TFs) play multifaceted roles in plant development and stress responses. Here, we show that strawberry (Fragaria × ananassa) FaRAV1 positively regulates anthocyanin accumulation during fruit ripening via a hierarchy activation processes. Dual-luciferase assay screening all fruit-expressed AP2/ERFs showed had the highest transcriptional promoter FaMYB10, key activator biosynthesis. Yeast one-hybrid electrophoretic mobility...
Significance A tomato fruit ripening–specific transcription factor, SlLOB1 predominantly influences cell wall–related gene regulation and textural changes during maturation thus is distinct from broadly acting ripening factors described to date that influence many processes. As such, an intermediate regulator primarily influencing a physiological subdomain of the overall transition.
Abstract Despite the initial success of some drugs and vaccines targeting COVID-19, understanding mechanism underlying SARS-CoV-2 disease pathogenesis remains crucial for development further approaches to treatment. Some patients with severe Covid-19 experience a cytokine storm display evidence inflammasome activation leading increased levels IL-1β IL-18; however, other reports have suggested reduced inflammatory responses Sars-Cov-2. In this study we examined effects Sars-Cov-2 envelope (E)...
Elevated hematocrit is associated with cardiovascular risk; however, the causality and mechanisms are unclear. The JAK2V617F (Jak2VF) mutation increases risk in myeloproliferative disorders clonal hematopoiesis. Jak2VF mice elevated WBCs, platelets, RBCs display accelerated atherosclerosis macrophage erythrophagocytosis. To investigate whether selective erythroid expression promotes atherosclerosis, we developed hyperlipidemic erythropoietin receptor Cre that express lineage (VFEpoR mice)....
Background: LNK/SH2B3 inhibits Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling by hematopoietic cytokine receptors. Genome-wide association studies have shown a common single nucleotide polymorphism in LNK (R262W, T allele) with neutrophilia, thrombocytosis, coronary artery disease. We that LNK(TT ) reduces function LNK-deficient mice display prominent platelet–neutrophil aggregates, accelerated atherosclerosis, thrombosis. Platelet–neutrophil interactions...
Background: Cholesterol loaded macrophage foam cells are a prominent feature of atherosclerotic plaques. Single-cell RNA sequencing has identified as TREM2 (triggering receptor expressed on myeloid 2) positive populations with low expression inflammatory genes, resembling the microglia neurodegenerative diseases. loading macrophages in vitro results activation LXR (liver X receptor) transcription factors and suppression genes. Methods: To test hypothesis that LXRs mediate anti-inflammatory...
Abstract Aims Neutrophil extracellular trap formation (NETosis) increases atherosclerotic plaque vulnerability and athero-thrombosis. However, mechanisms promoting NETosis during atherogenesis are poorly understood. We have shown that cholesterol accumulation due to myeloid cell deficiency of the transporters ATP Binding Cassette A1 G1 (ABCA1/G1) promotes NLRP3 inflammasome activation in macrophages neutrophils induces prominent plaques. investigated whether is a cell-intrinsic effect or...
To evaluate the association of systemic immune-inflammatory index (SII) and inflammatory response (SIRI) with clinical pathological features progression diabetic kidney disease (DKD).
Abstract Neutrophils kill bacteria generally through oxidative and nonoxidative mechanisms. Whereas much research has focused on the enzymes essential for neutrophil killing, little is known about regulatory molecules responsible such killing. In this study, we investigated role of olfactomedin 4 (OLFM4), an olfactomedin-related glycoprotein, in bactericidal capability host innate immunity. from OLFM4−/− mice have increased intracellular killing Staphylococcus aureus Escherichia coli vitro....
Hyperhomocysteinemia (HHcy) is associated with liver diseases such as fatty and hepatic fibrosis; however, the underlying mechanism still largely unknown. The current study aimed to explore signaling pathway involved in HHcy-induced steatosis (HS). C57BL/6 mice were fed a high-methionine diet (HMD) for 4 8 weeks establish HHcy mouse model. Compared chow diet, HMD induced elevated expression of CD36, acid transport protein. increased CD36 was activation aryl hydrocarbon receptor (AHR). In...
Abstract Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID-19) are urgently required. We studied an already-approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, severe acute respiratory syndrome 2 (SARS-CoV-2)-related virus. RNA-sequencing results showed virus perturbed expression multiple genes including those associated with cellular stress responses such as endoplasmic reticulum (ER) and heat shock factor 1 (HSF1)-mediated response, which...
Clonal hematopoiesis (CH) has emerged as an independent risk factor for atherosclerotic cardiovascular disease, with activation of macrophage inflammasomes a potential underlying mechanism. The NLRP3 (NLR family pyrin domain containing 3) inflammasome key role in promoting atherosclerosis mouse models
The deposition of cholesterol-rich lipoproteins in the arterial wall triggers macrophage inflammatory responses, which promote atherosclerosis. NLRP3 inflammasome aggravates atherosclerosis; however, cellular mechanisms connecting cholesterol accumulation to activation are poorly understood. We investigated loaded macrophages and atherosclerosis-prone Ldlr-/- mice with defects efflux. found that treated modified LDL or crystals, defective efflux promoting transporters ABCA1 ABCG1, leads...
The human olfactomedin 4 gene (OLFM4) encodes an olfactomedin-related glycoprotein. OLFM4 is normally expressed in a limited number of tissues, including the prostate, but its biological functions prostate are largely unknown. In this study, we found that messenger RNA was reduced or undetectable cancer tissues and cell lines. To study effects on progression, transfected PC-3 cells with to establish OLFM4-expressing clones. clones were have decreased proliferation invasiveness compared...
Chronic granulomatous disease (CGD) patients have recurrent life-threatening bacterial and fungal infections. Olfactomedin 4 (OLFM4) is a neutrophil granule protein that negatively regulates host defense against infection. The goal of this study was to evaluate the impact Olfm4 deletion on Staphylococcus aureus Aspergillus fumigatus in murine X-linked gp91phox-deficiency CGD model. We found intracellular killing vivo clearance S. aureus, as well resistance sepsis, were significantly...
Background and Purpose The ω‐3 polyunsaturated fatty acids (PUFAs) mediate protective effects on several metabolic disorders. However, the functions of their metabolites in early stage nonalcoholic liver disease (NAFLD) are largely unknown. Experimental Approach Mice were fed a control diet, high‐fat diet (HFD) or PUFA‐enriched HFD (ω3HFD) for 4 days phenotypes analysed. LC–MS/MS was used to determine eicosanoid profiles. Primary hepatocytes peritoneal macrophages mechanism study. Key...