A5015963885- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Virus-based gene therapy research
- Biofuel production and bioconversion
- Nanoparticle-Based Drug Delivery
- Microbial Metabolic Engineering and Bioproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Hepatitis B Virus Studies
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Enzyme Catalysis and Immobilization
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Advanced Drug Delivery Systems
- Dendrimers and Hyperbranched Polymers
- Anaerobic Digestion and Biogas Production
Shenzhen Institutes of Advanced Technology
2015-2022
Chinese Academy of Sciences
2012-2022
Sun Yat-sen University
2018
Institute for Biomedicine
2016
Guangzhou Institute of Energy Conversion
2012-2013
Jiangnan University
2011-2013
Abstract HBV covalently closed circular DNA (cccDNA) is drug-resistant and responsible for viral persistence. To facilitate the development of anti-cccDNA drugs, we developed a minicircle vector (MC)-based technology to produce large quantity recombined cccDNA (rcccDNA) resembling closely its wild-type counterpart both in structure function. The rcccDNA differed (wtcccDNA) only that it carried an extra 36-bp recombinant product attR upstream preC/C gene. Using procedure similar standard...
Minicircle DNA (mcDNA) is a kind of enhanced nonviral vector with excellent profiles in biosafety and transgene expression. Herein, we reported novel amphiphilic polymer comprising polyethylenimine(PEI) modified Poly(β-amino ester) PEI–PBAE(C16) for efficient mcDNA delivery vivo. The synthesized could condense into nanoscaled structure exhibited gene transfection ability without detectable cytotoxicity. Importantly, when injected mouse intraperitoneally, these nanocomplexes were able to...
DNA vectors for human gene therapy have to meet the efficacy and safety requirements. Minicircles (MCs), a class of optimized free plasmid backbone (PB) DNAs, emerged as promising candidates because their superior transgene expression profiles. However, existence impure including unrecombined MC producing (PP) PB circle, in products made using current technologies exceed limit. Here, we report development an enhanced triplex (TriD) technology eliminate almost all DNAs from products. To do...
An erythrocyte membrane based gene delivery system presents high transfection efficiency and negligible cytotoxicity.
Bispecific T-cell engagers (BiTEs) are single chain variable fragments, which could connect the surface antigen on cancer cells and CD<sub>3</sub> ligands T cells, then engage for immunotherapy.
Bispecific antibodies (BsAbs) are a class of promising anticancer immunotherapies. Among them, the US Food and Drug Administration (FDA)-approved blinatumomab (BLI) is very effective in eliminating minimum residual disease (MRD) acute lymphoblastic leukemia (ALL), resulting long-term remission many individuals. However, need for months-long intravenous delivery high cost limit its clinical acceptance. Here we demonstrate that these problems can be solved by BsAb expressed one intramuscular...
Breakthroughs have been achieved in cancer immunotherapies recently. Some patients experienced a durable remission or even cure by the treatments activating patients' own T cells. However, these single target technologies failed to result any therapeutic effect large percent of patients, and evidence suggests that further advances depend on effective strategy for coping with heterogeneity dynamics. We hypothesize minicircles, class optimized DNA vectors, are able cope problems; is apply...