A5074528927- Sulfur Compounds in Biology
- Liver Disease Diagnosis and Treatment
- Folate and B Vitamins Research
- Diet, Metabolism, and Disease
- Amino Acid Enzymes and Metabolism
- Alcohol Consumption and Health Effects
- Adipose Tissue and Metabolism
- Glutathione Transferases and Polymorphisms
- Nutritional Studies and Diet
- Fibroblast Growth Factor Research
- Pancreatic function and diabetes
- Advanced Glycation End Products research
- GABA and Rice Research
- Metabolomics and Mass Spectrometry Studies
- Mast cells and histamine
- Renin-Angiotensin System Studies
- Polyamine Metabolism and Applications
- Neuroscience of respiration and sleep
- Diabetes and associated disorders
- Neonatal Health and Biochemistry
- Eicosanoids and Hypertension Pharmacology
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Regulation of Appetite and Obesity
- Biochemical effects in animals
The University of Texas Medical Branch at Galveston
2016-2021
University of Toronto
2018-2020
Lakehead University
2011-2016
Thunder Bay Regional Research Institute
2011-2016
University of Saskatchewan
2009-2013
St. Joseph’s Healthcare Hamilton
2013
McMaster University
2013
Cystathionine γ-lyase (CSE) produces hydrogen sulfide (H2S) in the cardiovascular system. The deficiency of CSE mice leads to a decreased endogenous H2S level, an age-dependent increase blood pressure, and impaired endothelium-dependent vasorelaxation. To date, there is no direct evidence for causative role altered metabolism atherosclerosis development.Six-week-old gene knockout wild-type were fed with either control chow or atherogenic paigen-type diet 12 weeks. Plasma lipid profile...
Mounting evidence has established hydrogen sulfide (H(2)S) as an important gasotransmitter with multifaceted physiological functions. The aim of the present study was to investigate role H(2)S on glucose utilization, glycogen synthesis, well gluconeogenesis in both HepG(2) cells and primary mouse hepatocytes. Incubation NaHS (a donor) impaired uptake storage via decreasing glucokinase activity. Adenovirus-mediated cystathionine γ-lyase (CSE) overexpression increased endogenous production...
Colon cancer cells contain high levels of cystathionine-beta-synthase (CBS). Its product, hydrogen sulfide (H2S) promotes the growth and proliferation colorectal tumor cells. In order to improve antitumor efficacy prototypical CBS inhibitor aminooxyacetic acid (AOAA), we have designed synthesized YD0171, a methyl ester derivative AOAA. The antiproliferative effect YD0171 exceeded potency AOAA in HCT116 human colon esterase paraoxon prevented cellular inhibition activity by YD0171. suppressed...
γ-Aminobutyric acid (GABA) administration has been shown to increase β-cell mass, leading a reversal of type 1 diabetes in mice. Whether GABA any effect on β cells healthy and prediabetic/glucose-intolerant obese mice remains unknown. In the present study, we show that oral (ad libitum) indeed increased pancreatic which led modest enhancement insulin secretion glucose tolerance. However, treatment did not further insulin-positive islet area high fat diet-fed was unable prevent or reverse...
To investigate the regulation of hepatic glucose production by cystathionine γ-lyase (CSE)-generated hydrogen sulfide (H2S) in under physiological conditions.We found that CSE knockout (KO) mice had a reduced rate gluconeogenesis, which was reversed administration NaHS (an H2S donor) (i.p.). Interestingly, isolated KO hepatocytes exhibited glycemic response to chemical-induced activation cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) and glucocorticoid pathways compared with...
Glycine and β-alanine activate glycine receptors (GlyRs), with known to enhance insulin secretion from pancreatic islet β cells, primarily through GlyR activation. However, the effects of activation on cell proliferation have not been examined. Here, we aim investigate potential proliferative islets. In vitro experiments mouse human islets revealed that β-alanine, via activation, stimulated cells α without affecting or glucagon secretion. Further analysis indicated involvement...
Hydrogen sulfide (H(2)S) is a gasotransmitter with multifaceted physiological functions, including the regulation of glucose metabolism. Methylglyoxal (MG) an intermediate metabolism and plays important role in pathogenesis insulin resistance syndromes. In present study, we investigated effect MG on H(2)S synthesis interaction between these two endogenous substances. cultured vascular smooth muscle cells (VSMCs), (10, 30, 50 microM) significantly decreased cellular levels...
Upregulation of hydrogen sulfide (H2S) biosynthesis, at least in part related to the upregulation cystathionine β-synthetase (CBS) cancer cells, serves as a tumor-promoting factor and has emerged possible molecular target for antitumor drug development. To facilitate future clinical translation, we have synthesized variety novel CBS-targeting, esterase-cleavable prodrugs based on structure prototypical CBS inhibitor aminooxyacetic acid (AOAA). The pharmacological properties these compounds...
We have previously reported that hydrogen sulfide (H2S), a gasotransmitter and vasodilator has cytoprotective properties against methylglyoxal (MG), reactive glucose metabolite associated with diabetes hypertension. Recently, H2S was shown to up-regulate peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, key gluconeogenic regulator enhances the gene expression of rate-limiting enzyme, fructose-1,6-bisphosphatase (FBPase). Thus, we sought determine whether MG levels enzymes...
To examine the mechanism of action γ-aminobutyric acid (GABA) on β-cell proliferation and investigate if co-treatment with Ly49, a novel GABA type A receptor positive allosteric modulator (GABAA -R PAM), amplifies this effect.Human or mouse islets were co-treated for 4-5 days selected cell signalling pathway modulators. Immunofluorescence was used to determine protein co-localization, number proliferation, islet size. Osmotic minipumps surgically implanted in mice assess Ly49 effects...