A5067476138- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Sarcoma Diagnosis and Treatment
- Testicular diseases and treatments
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Endometriosis Research and Treatment
- Glioma Diagnosis and Treatment
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Photoacoustic and Ultrasonic Imaging
- Telomeres, Telomerase, and Senescence
- Olfactory and Sensory Function Studies
- Reproductive System and Pregnancy
- Biochemical Analysis and Sensing Techniques
- MicroRNA in disease regulation
- Ultrasound and Hyperthermia Applications
- Mathematical Biology Tumor Growth
- Pluripotent Stem Cells Research
- Immune cells in cancer
University Health Network
2004-2023
Princess Margaret Cancer Centre
2012-2023
Ontario Genomics
2013-2023
Ontario Institute for Cancer Research
2002-2012
Mount Sinai Hospital
2011
Lunenfeld-Tanenbaum Research Institute
2011
University of Toronto
1996-2005
The liver is the largest solid organ in body and critical for metabolic immune functions. However, little known about cells that make up human its microenvironment. Here we report a map of cellular landscape using single-cell RNA sequencing. We provide transcriptional profiles 8444 parenchymal non-parenchymal obtained from fractionation fresh hepatic tissue five livers. Using gene expression patterns, flow cytometry, immunohistochemical examinations, identify 20 discrete cell populations...
A fundamental principle guiding the publication of scientific results is that data supporting any scholarly work must be made fully available to research community, in a form allows basic conclusions evaluated independently. In context molecular biology, this has typically meant authors paper describing newly sequenced genome, gene, or protein deposit primary permanent, public repository, such as sequence databases maintained by DNA Data Bank Japan (DDBJ), European Bioinformatics Institute...
γ-Aminobutyric acid (GABA) administration has been shown to increase β-cell mass, leading a reversal of type 1 diabetes in mice. Whether GABA any effect on β cells healthy and prediabetic/glucose-intolerant obese mice remains unknown. In the present study, we show that oral (ad libitum) indeed increased pancreatic which led modest enhancement insulin secretion glucose tolerance. However, treatment did not further insulin-positive islet area high fat diet-fed was unable prevent or reverse...
An effective tool for the global analysis of both DNA methylation status and protein–chromatin interactions is a microarray constructed with sequences containing regulatory elements. One type array suited this purpose takes advantage strong association between CpG Islands (CGIs) gene regions. We have obtained 20 736 clones from CGI Library used these to construct arrays. The utility library requires proper annotation assessment clones, including content, genomic origin proximity neighboring...
Abstract Medulloblastoma (MB) is defined by four molecular subgroups (Wnt, Shh, Group 3, 4) with Wnt MB having the most favorable prognosis. Since prior reports have illustrated antitumorigenic role of activation in Shh MB, we aimed to assess effects activated canonical signaling 3 and 4 MBs. By using primary patient-derived brain tumor-initiating cell (BTIC) lines, characterize differences capacity Wnt, MB. With single RNA-seq technology, demonstrate presence rare Wnt-active cells non-Wnt...
Radiotherapy is a widely used cancer treatment. However, understanding how ionizing radiation affects tumor cells and their vasculature, particularly at cellular, subcellular, genetic, protein levels, has been limited by an inability to visualize the response of these interdependent components within solid tumors over time in vivo. Here we describe new preclinical experimental platform combining intravital multimodal optical microscopy for cellular-level longitudinal imaging, small animal...
Sodium salicylate has long been known to be an inducer of the heat shock puffs and presumably gene transcription in polytene chromosomes Drosophila salivary gland cells. Stress-induced genes is mediated by factor as Heat Shock Factor (HSF). In yeast, sodium reported induce DNA binding HSF but not itself, similar findings have human This apparent discrepancy induction certain aspects response between these organisms prompted us carefully reexamine cells third instar larvae tissue culture...
ABSTRACT The induction of the heat shock genes in eukaryotes by and other forms stress is mediated a transcription factor known as 1 (HSF1). HSF1 present unstressed metazoan cells monomer with low affinity for DNA, upon exposure to it converted an ‘active’ homotrimer that binds promoters high induces their transcription. conversion its active form hypothesized be multistep process involving physical changes molecule possible translocation from cytoplasm nucleus. While all studies date have...
The precise relationship between epigenetic alterations and telomere dysfunction is still an extant question. Previously, we showed that eroded telomeres lead to differentiation instability in murine embryonic stem cells (mESCs) via DNA hypomethylation at pluripotency-factor promoters. Here, uncovered telomerase reverse transcriptase null (Tert-/-) mESCs exhibit genome-wide chromatin accessibility gene expression during differentiation. These changes were accompanied by increase of H3K27me3...
Recurrence of solid tumors renders patients vulnerable to advanced, treatment-refractory disease state with mutational and oncogenic landscape distinctive from initial diagnosis. Improving outcomes for recurrent cancers requires a better understanding cell populations that expand the post-therapy, minimal residual (MRD) state. We profile barcoded tumor stem through therapy at initiation, MRD, recurrence in our therapy-adapted, patient-derived xenograft models glioblastoma (GBM). Tumors show...
ABSTRACT Over the last two decades, molecular biology has been changed by introduction of high-throughput technologies. Data sharing requirements have prompted establishment persistent data archives. A standardized approach for recording and managing these was first proposed in Minimal Information About a Microarray Experiment (MIAME) guidelines. The about high throughput nucleotide Sequencing (MINSEQE) proposal introduced 2008 as logical extension guidelines to next-generation sequencing...
To identify differentially expressed genes between relapsed and non-relapsed clinical stage I testicular germ cell tumours (TGCTs).We reviewed patients with non-seminoma seminoma from an institutional database (2000-2012) who were managed by active surveillance. Patients defined as being relapse-free after 2 3 years of surveillance, respectively. RNA extraction gene expression analysis was performed on archival primary tumour samples gene-set enrichment (GSEA) conducted in order to...
Abstract Recurrence of solid tumors renders patients vulnerable to a distinctly advanced, highly treatment-refractory disease state that has an increased mutational burden and novel oncogenic drivers not detected at initial diagnosis. Improving outcomes for recurrent cancers requires better understanding cancer cell populations expand from the post-therapy, minimal residual (MRD) state. We profiled barcoded tumor stem through therapy initiation/engraftment, MRD recurrence in our...
Abstract Introduction: The molecular characterization of patients who exhibit a unique response to treatments that are not effective in most patients, so called “exceptional responders”, can shed light on the biological underpinnings and provide rationale for selection future same treatment. Here we present genomic 22-year-old female patient presented with an unclassifiable kidney cancer diffuse bilateral lung metastases. primary tumour was resected platinum plus gemcitabine therapy achieved...
493 Background: Genomic signatures may compliment pathological features in identifying appropriate patients who benefit from adjuvant therapy Stage I (SI) TGCT. This study aimed to identify a gene expression pattern differentiate between relapsed (R) and non-relapsed (NR) SI Methods: Patients with non-seminoma (NS) seminoma (S) were identified an institutional database 2000 2012. All managed active surveillance. NR-NS NR-S defined as having no evidence of relapse after 2 3 years surveillance...