A5045261152- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Pharmacological Effects of Natural Compounds
- Plant-based Medicinal Research
- Zoonotic diseases and public health
- Vector-borne infectious diseases
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Bee Products Chemical Analysis
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Ethnobotanical and Medicinal Plants Studies
- Bacterial Identification and Susceptibility Testing
- Botanical Research and Chemistry
- Insect and Pesticide Research
- Viral gastroenteritis research and epidemiology
- Heat shock proteins research
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Agriculture and Biological Studies
- Antimicrobial Peptides and Activities
- Entomopathogenic Microorganisms in Pest Control
- Viral Infections and Outbreaks Research
State Research Center for Applied Microbiology and Biotechnology
2016-2025
Pushchino State Institute of Natural Sciences
2022
Ministry of Health
2009
Brigham and Women's Hospital
2005
N.D. Zelinsky Institute of Organic Chemistry
2005
Russian Academy of Sciences
2005
Yersinia pestis, the pathogen of plague, has greatly influenced human history on a global scale. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR), an element participating in immunity against phages' invasion, is composed short repeated sequences separated by unique spacers and provides basis spoligotyping technology. In present research, three CRISPR loci were analyzed 125 strains Y. pestis from 26 natural plague foci China, former Soviet Union Mongolia analyzed, for...
Yersinia pestis spread throughout the Americas in early 20th century, and it occurs predominantly as a single clone within this part of world. However, Eurasia parts Africa there is significant diversity among Y. strains, which can be classified into different biovars (bv.) and/or subspecies (ssp.), with bv. orientalis/ssp. most closely related to American clone. To determine one aspect relatedness these isolates, structure lipopolysaccharide (LPS) four wild-type LPS-mutant Eurasian/African...
The species Yersinia pestis is commonly divided into three classical biovars, Antiqua, Medievalis, and Orientalis, belonging to subspecies pathogenic for human the (atypical) non-human biovar Microtus (alias Pestoides) including several non-pestis subspecies. Recent progress in molecular typing methods enables large-scale investigations population structure of this species. It now possible test hypotheses about its evolution which were proposed decades ago. For instance biovars different...
ABSTRACT φA1122 is a T7-related bacteriophage infecting most isolates of Yersinia pestis , the etiologic agent plague, and used by CDC in identification Y. . infects grown both at 20°C 37°C. Wild-type pseudotuberculosis strains are also infected but only when Since expresses rough lipopolysaccharide (LPS) missing O-polysaccharide (O-PS) expression O-PS largely suppressed temperatures above 30°C, it has been assumed that phage receptor LPS. We present here several lines evidence to support...
16S rRNA genes sequencing has been used for routine species identification and phylogenetic studies of bacteria. However, the high sequence similarity between some heterogeneity within copies at intragenomic level could be a limiting factor discriminatory ability. In this study, we aimed to compare sequences genome-based analysis (core SNPs ANI) non-pathogenic Yersinia. We complete draft genomes 373 Yersinia strains from NCBI Genome database. The taxonomic affiliations 34 based on core ANI...
Background/Objectives Bacterial ghosts (BGs), non-living empty envelopes of bacteria, are produced either through genetic engineering or chemical treatment retaining the shape their parent cells. BGs considered vaccine candidates, promising delivery systems, and adjuvants. The practical use in development for humans is limited because concerns about preservation viable bacteria BGs. Methods: To increase efficiency Klebsiella pneumoniae BG formation and, accordingly, to ensure maximum killing...
Background/Objectives: Yersinia pestis is an important zoonotic pathogen responsible for the rare but deadly disease of people with bubonic, septic, or pneumonic forms plague. The emergence multidrug-resistant Y. strains has attracted more and researchers’ attention to search molecular targets antivirulence therapy, including anti-nutritional-virulence therapy. glnALG operon plays a crucial role in regulating nitrogen content within bacterial cell. This codes three genes: structural gene...
ABSTRACT Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation different subspecies strains Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37°C; fleas, 25°C; or winter hibernation, 6°C) affects LPS composition structure. We tested susceptibilities Y. grown these PMB killing. Both properties varied significantly in...
Yersinia pestis, a Gram-negative bacterium and the etiologic agent of plague, has evolved from pseudotuberculosis, cause mild enteric disease. However, molecular biological mechanisms how Y. pseudotuberculosis to such remarkably virulent pathogen, are not clear. The ability initiate rapid bacterial dissemination is characteristic hallmark pestis infection. A distinguishing between two species that strains possess an O-antigen lipopolysaccharide (LPS) while lost during evolution therefore...
Yersinia pestis undergoes an obligate flea-rodent-flea enzootic life cycle. The rapidly fatal properties of Y. are responsible for the organism's sustained survival in natural plague foci. Lipopolysaccharide (LPS) plays several roles pathogenesis, prominent among them being resistance to host immune effectors and induction a septic-shock state during terminal phases infection. LPS is acylated with 4-6 fatty acids, number varying growth temperature affecting molecule's toxic properties....
The etiologic agent of plague, Yersinia pestis, includes two subspecies, which Y. pestis subsp. microtus contains the strains that cause only occasional diseases in humans are not accompanied by human-to-human transmission. Here, we report draft genome sequences 19 (across 6 biovars microtus).
Two isogenic sets of Yersinia pestis strains were generated, composed wild-type 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or whole operon, as well restored ability temperature- pH-dependent synthesis adhesion pili constitutive production 6 antigen. The generated by site-directed mutagenesis psa operon subsequent complementation trans. It was shown loss antigen did not influence Y. virulence average survival time...