A5083617874- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
- Escherichia coli research studies
- Pharmacological Effects of Natural Compounds
- Bee Products Chemical Analysis
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Viral gastroenteritis research and epidemiology
- Plant-based Medicinal Research
- Zoonotic diseases and public health
- Agriculture and Biological Studies
- Healthcare and Venom Research
- Insect and Pesticide Research
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Animal Ecology and Behavior Studies
- Bacteriophages and microbial interactions
- Ethnobotanical and Medicinal Plants Studies
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
State Research Center for Applied Microbiology and Biotechnology
2011-2024
Two isogenic sets of Yersinia pestis strains were generated, composed wild-type 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or whole operon, as well restored ability temperature- pH-dependent synthesis adhesion pili constitutive production 6 antigen. The generated by site-directed mutagenesis psa operon subsequent complementation trans. It was shown loss antigen did not influence Y. virulence average survival time...
It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of outer membrane protease Pla, while epidemic highly virulent strains possessed only T259 Pla isoform. Our sequence analysis pla gene from 118 subsp. microtus revealed was present exclusively in providing a convictive evidence more ancestral origin this Analysis effects I259T polymorphism on intrinsic disorder propensity mutation slightly increases C-terminal tail and makes...
Data on VNTR typing of anthrax strains from SRC AMB collection are presented. 25 Bacillus anthracis different origin and isolated in territories have been analyzed. Application the scheme P.Keim et al . enabled to correlate Russian with other collections. 4 new alleles: Dakkar-VrrA(296), 157-VrrB2(180), Dakkar-VrrC2(478) M-29-pXO1(138) were determined. Major part refers A group (the size VrrB1 fragment is 229 b.p.) only two (1051 157) possess b.p. The following collection: 1 Kolomna, Ch-7,...
Oral vaccines are drawing more attention due to their ease of administration, lesser invasiveness, and greater safety in general. The review discusses the benefits oral vaccination stimulating humoral cellular immune responses at systemic mucosal level provide expanded longer-lasting protection. Aspects related structure intestine immunological recognition antigen during transformation process after penetration into analyzed. Approaches used improve effectiveness considered. Problems such as...
The causative agent of plague, Yersinia pestis, is a highly virulent bacterial pathogen and potential bioweapon. Depending on the route infection, two prevalent forms disease — bubonic pneumonic, are known. latter featured by high fatality rate. Mortality in untreated plague patients reaches up to 40—60%, whereas pneumonic always lethal. development infectious process susceptible host accounted for whole set pathogenicity factors displaying various functional modalities being expressed...
Antibiotic therapy of plague is hampered by the recent isolation Yersinia pestis strain resistant to all antibiotics recommended for cure. This has constrained a quest new antimicrobials taking aim at alternative targets. Recently Y. cysteine protease YopT been explored as potential drug target. Targets conserved in pathogen populations should be more efficacious; therefore, we evaluated intraspecies variability yopT genes and their products. 114 isolates were screened. Only two full-size...
Designing of new means for the specific prevention plague, especially protein subunit vaccines, is impossible without studying role individual antigens in manifestation pathogenic and immunogenic properties Yersinia pestis . The aim present study was to determine antibody levels Y. guinea pigs that survived infection with sub-lethal doses virulent plague agent strains using enzyme immunoassay (ELISA). Materials methods. Guinea were inoculated subcutaneously 30 CFU wild type subsp. Pestis...
The technique of site-directed mutagenesis psa operon encoding the synthesis pH6 antigen (adhesion pili), followed by trans-complementation procedure were used to construct two sets isogenic Yersinia pestis strains including wild type 231 and I-1996, their non-polar - mutants containing deletions in structural psA gene or entire operon, as well which could recover capability for temperature- pH-dependent adhesion pili constitutive production. loss ability synthesize its production was shown...
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HtpG (high-temperature protein G) is a bacterial homologue of the highly conserved molecular chaperone Hsp90 eukaryotes, which plays an important role in protection against stress many species. The htpG gene encoding synthesis high-temperature prokaryotic G pathogenesis infections still unclear. aim this work to study functional importance plague. Materials and methods. Isogenic Yersinia pestis sets based on attenuated virulent strains differing presence (YPO3119) were generated with help...