A5026593822- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Zoonotic diseases and public health
- Bee Products Chemical Analysis
- Pharmacological Effects of Natural Compounds
- Enzyme Production and Characterization
- Advanced Chemical Sensor Technologies
- thermodynamics and calorimetric analyses
- Protein purification and stability
- Agriculture and Biological Studies
- Probiotics and Fermented Foods
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- Microbial infections and disease research
- Insect and Pesticide Research
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Analytical Chemistry and Chromatography
- Botanical Research and Chemistry
- Vibrio bacteria research studies
- Ethnobotanical and Medicinal Plants Studies
- Travel-related health issues
State Research Center for Applied Microbiology and Biotechnology
2011-2023
Increases in the prevalence of antibiotic-resistant strains Staphylococcus aureus have elicited efforts to develop novel antimicrobials treat these drug-resistant pathogens. One potential treatment repurposes lytic enzymes produced by bacteriophages as antimicrobials. The phage Twort endolysin (PlyTW) harbors three domains, a cysteine, histidine-dependent amidohydrolases/peptidase domain (CHAP), an amidase-2 and SH3b-5 cell wall binding (CBD). Our results indicate that CHAP alone is...
Francisella tularensis, a small Gram-negative bacterium, is capable of infecting wide range animals, including humans, and causes plague-like disease called tularemia-a highly contagious with high mortality rate. Because these characteristics, F. tularensis considered potential agent biological terrorism. Currently, divided into four subspecies, which differ in their virulence geographic distribution. Two them, subsp. (primarily found North America) holarctica (widespread across the Northern...
It has recently been shown that the NlpD lipoprotein is essential to Yersinia pestis virulence and subcutaneous administration of nlpD mutant could protect mice against bubonic pneumonic plague better than EV vaccine strain [PLoS One 2009. V. 4. № 9. e7023]. In this study, similar mutants were generated on basis other Y. parent strains, including strains from subspecies microtus, which avirulent guinea pigs humans. Comparative testing confirmed immunization with induces immunity 105 times...
Yersinia pestis Caf1 is a multifunctional protein responsible for antiphagocytic activity and key protective antigen. It generally conserved between globally distributed Y. strains, but subsp. microtus biovar caucasica strains circulating within populations of common voles in Georgia Armenia were reported to carry single substitution alanine serine. We investigated polymorphism the sequences among other which have limited virulence guinea pigs humans. Sequencing caf1 genes from 119 belonging...
It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of outer membrane protease Pla, while epidemic highly virulent strains possessed only T259 Pla isoform. Our sequence analysis pla gene from 118 subsp. microtus revealed was present exclusively in providing a convictive evidence more ancestral origin this Analysis effects I259T polymorphism on intrinsic disorder propensity mutation slightly increases C-terminal tail and makes...
Plague has been the cause of three pandemics and led to death millions people. is a typical zoonosis caused by Yersinia pestis that circulates in populations wild rodents inhabiting natural plague foci on all continents except for Australia. Transmission provided flea bites. Circulation Y. supported numerous pathogenicity factors. This review explores one them, plasminogen activator Pla. protein representatives omptins, family enterobacterial outer membrane proteases are responsible...
Evaluated was the ability of plague microbe antigens F1 and V to activate specifically in vitro system subpopulations T-lymphocytes BALB/c mice immunized against plague. The level CD69 expression at lymphocytes surface proposed be evaluated as marker activation. Expression early activation T-helpers cytotoxic lymphocytes, reciprocated by stimulation with Y. pestis F1, correlated intensity anti-plague postvaccinal immunity induced immunization or F1and mixtures.
Plague is a zoonosis caused by gram-negative bacteria Yersinia pestis, which, as rule, transmitted to humans from septicemic rodents the bites of infected fleas. This microbe killed more people than all wars in human history. Y. pestis circulation natural plague foci ensured whole number pathogenicity factors with differing functional orientation. review devoted one them, capsular antigen (F1 or Caf1). The history its discovery and studying genetic control, biosynthesis, isolation...
Obtained are the immunomagnetic particles with immobilized highly-specific monoclonal antibodies FB 11-x to Francisella tularensis. Their usage in enzyme-linked immunoassays made it possible detect 10 5 - 6 CFU/ml of F. The binding bacterial cells IMP was confirmed by results fluorescent microscopy.
The aim of the work was to put forward methods for direct quantitative determination content Yersinia pestis and Rickettsia raoultii protein antigens in preparations various prototypes subunit vaccines. Materials methods. Y. LcrV Caf1 enclosed substance molecular microencapsulated plague vaccine (MMPV) separately, microcrystals amino acids co-precipitated with proteins were used as model antigens. R. Adr2, OmpB24, YbgF adsorbed on prototype rickettsia vaccine. release from MMPV microcapsules...
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