A5052691073- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Biochemical Analysis and Sensing Techniques
- Protein Tyrosine Phosphatases
- Genomics and Phylogenetic Studies
- Diet, Metabolism, and Disease
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- Pituitary Gland Disorders and Treatments
- Drug Transport and Resistance Mechanisms
- Caveolin-1 and cellular processes
- Adrenal and Paraganglionic Tumors
- Ubiquitin and proteasome pathways
- Neural Networks and Applications
- Fatty Acid Research and Health
- Infant Nutrition and Health
- Computational Physics and Python Applications
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Molecular Biology Techniques and Applications
- Glioma Diagnosis and Treatment
- Reproductive biology and impacts on aquatic species
- Genetics and Physical Performance
Medical College of Wisconsin
2019-2024
Thomas Jefferson University
2024
Cancer Research Center
2024
Normandie Université
2023
National Institute of Diabetes and Digestive and Kidney Diseases
2014-2019
National Institutes of Health
2014-2019
Unité de Glycobiologie Structurale et Fonctionnelle
2009-2016
Centre National de la Recherche Scientifique
2009-2016
Université de Lille
2009-2016
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013-2014
Dysfunctions in Wnt signaling increase β-catenin stability and are associated with cancers, including colorectal cancer. In addition, degradation is decreased by nutrient-dependent O-GlcNAcylation. Human colon tumors colons from mice fed high-carbohydrate diets exhibited higher amounts of O-GlcNAc relative to healthy tissues a standard diet, respectively. Administration the O-GlcNAcase inhibitor thiamet G also increased colonic expression β-catenin. By ETD-MS/MS, we identified 4...
Non-nutritive sweeteners (NNS) are marketed as sugar alternatives providing sweet taste with few or no calories. Yet their consumption has been linked to metabolic dysfunction and changes in the gut microbiome. NNS exposure mostly originates from diet beverages sweetener packages adults breastmilk infants. Consequences of early life remain largely unknown. We exposed pregnant lactating mice (sucralose, acesulfame-K) at doses relevant for human consumption. While pups' was low, were drastic,...
Nutrient-driven O-GlcNAcylation is strikingly abundant in the brain and has been linked to development neurodegenerative disease. We selectively targeted O-GlcNAcase (Oga) gene mouse define role of O-GlcNAc cycling central nervous system. Brain knockout animals exhibited dramatically increased levels pleiotropic phenotypes, including early-onset obesity, growth defects, metabolic dysregulation. Anatomical defects Oga included delayed differentiation neurogenesis as well abnormal...
The short half-life protooncogene β-catenin acquires a remarkable stability in large subset of cancers, mainly from mutations affecting its proteasomal degradation. In this sense, colorectal cancers (CRC) form group pathologies which early steps development are characterized by an aberrant expression and uncontrolled proliferation epithelial cells. Diet has long been described as influence the emergence CRC, but molecular events that link metabolic disorders CRC remain elusive. Part...
The post-translational modification of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is regulated a unique couple enzymes. O-GlcNAc transferase (OGT) transfers the GlcNAc residue from UDP-GlcNAc, final product hexosamine biosynthetic pathway (HBP), whereas O-GlcNAcase (OGA) removes it. This study and others show that OGT O-GlcNAcylation levels are increased in cancer cell lines. In context we studied effect silencing colon lines HT29 HCT116 primary line CCD841CoN. Herein report...
Abstract O-GlcNAcylation is a dynamic post-translational modification that involves the addition of N-acetylglucosamine (GlcNAc) to serine and threonine residues proteins. Over past four decades, this has become increasingly recognized as having critical influence in field endocrinology. The carefully controlled hormonal input for regulating sleep, mood, response stress, growth development, metabolism are often associated with O-GlcNAc-dependent signaling. As protein patterns heavily...
Nuclear and cytoplasmic O-GlcNAc transferase (OGT) is a unique universally expressed enzyme catalyzing O-GlcNAcylation of thousands proteins. Although OGT interferes with many crucial intracellular processes, including cell cycle, only few studies have focused on elucidating the precise role glycosyltransferase during cycle entry. We first demonstrated that starved MCF7 cells reincubated serum quickly induced significant increase concomitantly to activation PI3K MAPK pathways....
Lipid microdomains (rafts) are cholesterol-enriched dynamic ordered lipid domains belonging to cell membranes involved in diverse cellular functions, including signal transduction, membrane trafficking, and infection. Many studies have reported relationships between insulin signaling rafts. Likewise, links O-GlcNAcylation also been described. However, the potential connection O-GlcNAc raft dynamics remains unexplored. Here we show that enzyme creates this modification, transferase (OGT),...
O-GlcNAcylation is a dynamic modulator of signaling pathways, equal in magnitude to the widely studied phosphorylation. With rapid development tools for its detection at single protein level, O-GlcNAc modification rapidly emerged as novel diagnostic and therapeutic target human diseases. Yet, mapping O-GlcNAcome various tissues essential generating relevant biomarkers. In this study, we used banked tissue sample source identify O-GlcNAcylated targets Using term placentas, propose (1) method...
O-GlcNAc Transferase (OGT) catalyzes protein O-GlcNAcylation, an abundant and dynamic nuclear cytosolic modification linked to epigenetic regulation of gene expression. The steady-state levels are influenced by extracellular glucose concentrations suggesting that O-GlcNAcylation may serve as a metabolic sensor. Intriguingly, human OGT is located on the X-chromosome (Xq13) close X-inactivation center (XIC), be controlled dosage compensation. In female cells, compensation accomplished...
Non-nutritive sweeteners (NNS) are popular sugar replacements used in foods, beverages, and medications. Although NNS considered safe by regulatory organizations, their effects on physiological processes such as detoxification incompletely understood. Previous studies revealed that the sucralose (Sucr) altered P-glycoprotein (PGP) expression rat colon. We also demonstrated early-life exposure to Sucr acesulfame potassium (AceK) compromises mouse liver detoxification. Building upon these...
Abstract Post-translational modifications (PTMs) are ubiquitous and essential for protein function signaling, motivating the need sustainable benefit open models of web databases. Highly conserved O-GlcNAcylation is a case example one most recently discovered PTMs, investigated by growing community. Historically, details about O-GlcNAcylated proteins sites were dispersed across literature in non-O-GlcNAc-focused, rapidly outdated or now defunct In first effort to fill gap, we published human...
The widespread use of 17α-ethinylestradiol (EE2), and other estrogenic endocrine disruptors, results in a continuous release compounds into aquatic environments. Xenoestrogens may interfere with the neuroendocrine system organisms produce various adverse effects. aim present study was to expose European sea bass larvae (Dicentrarchus labrax) EE2 (0.5 50 nM) for 8 d determine expression levels brain aromatase (cyp19a1b), gonadotropin-releasing hormones (gnrh1, gnrh2, gnrh3), kisspeptins...