A5100615233- Adipose Tissue and Metabolism
- DNA Repair Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Food Quality and Safety Studies
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Paraquat toxicity studies and treatments
- Hedgehog Signaling Pathway Studies
- Pesticide Exposure and Toxicity
- Metabolism, Diabetes, and Cancer
- Gut microbiota and health
- Sirtuins and Resveratrol in Medicine
- Nutrition, Health and Food Behavior
- CRISPR and Genetic Engineering
- Renal and related cancers
- Bone Tissue Engineering Materials
- Ginseng Biological Effects and Applications
- Bone health and osteoporosis research
- Bone and Dental Protein Studies
- Protein Tyrosine Phosphatases
- Biochemical effects in animals
- Genomics and Chromatin Dynamics
- Genomics, phytochemicals, and oxidative stress
- Essential Oils and Antimicrobial Activity
- Cancer-related gene regulation
Soonchunhyang University
2014-2024
Jeonbuk National University
2015
Wonkwang University
2011-2013
Seoul National University Hospital
2013
Dongguk University Ilsan Hospital
2013
Korea University
2011
National Institutes of Health
2002-2008
National Institute of Environmental Health Sciences
2002-2006
Triangle
2002-2006
Laboratoire de Radiopharmaceutiques Biocliniques
2006
PURPOSE: To determine whether the marrow edema around focal osteonecrosis on magnetic resonance (MR) images is associated with clinical symptoms. MATERIALS AND METHODS: Thirty-three patients 37 hips showing early stage of femoral head were followed up at 3-month intervals evaluation, conventional radiography, and serial MR imaging. RESULTS: Seven (50%) 14 symptomatic showed initial images, whereas only one (4%) 23 asymptomatic (P < .01). Six (86%) seven that moderately to severely painful...
In this study, we describe the generation and partial characterization of Krüppel-like zinc finger protein Glis3 mutant (Glis3(zf/zf)) mice. These mice display abnormalities very similar to those patients with neonatal diabetes hypothyroidism syndrome, including development polycystic kidney disease. We demonstrate that localizes primary cilium, suggesting is part a cilium-associated signaling pathway. Although Glis3(zf/zf) form normal cilia, renal cysts contain relatively fewer cells...
To obtain insight into the physiological functions of Krüppel-like zinc finger protein Gli-similar 2 (Glis2), mice deficient in Glis2 expression were generated. mutant (Glis2mut) exhibit significantly shorter life spans than do littermate wild-type (WT) due to development progressive chronic kidney disease with features resembling nephronophthisis. Glis2mut develop severe renal atrophy involving increased cell death and basement membrane thickening proximal convoluted tubules. This is...
The Krüppel-like zinc finger protein Gli-similar 3 (Glis3) plays a critical role in pancreatic development and has been implicated syndrome with neonatal diabetes hypothyroidism (NDH). In this study, we examine three steps the mechanism of transcriptional regulation by Glis3: its translocation to nucleus, DNA binding activity. We demonstrate that putative bipartite nuclear localization signal is not required, but tetrahedral configuration fourth essential for Glis3. identify...
Abstract Delphinidin (Del) is a major anthocyanin that widely found in pigmented fruits and vegetables. Herein, we investigated the antiadipogenic effect molecular mechanism by which Del affects 3T3‐L1 preadipocyte differentiation. We effectively reduced intracellular lipid accumulation promoted lipolysis while regulating expression of adipogenic transcription factors their target genes. This lowering was largely limited to early phase adipogenesis, governed delayed cell cycle progression...
Abstract Methyl gallate (MG) is a derivative of gallic acid and potent antioxidant. In this study, we confirmed that MG treatment effectively inhibits lipid accumulation, which occurred mostly in the early stages adipogenesis. We also showed shortly after adipogenic induction, facilitated G0/G1 cell cycle arrest. Mechanistic studies revealed inhibited ERK1/2 phosphorylation, key regulator G1‐ to S‐phase transition. Furthermore, prevented hormonal stimuli‐induced inhibition cyclin‐dependent...
In this study, we describe the identification and characterization of a novel Krüppel-like protein named Gli-similar 1 (Glis1). The Glis1 gene encodes an 84.3-kDa proline-rich protein. Its five tandem zinc finger motifs exhibit highest homology with those members Gli Zic subfamilies proteins. was mapped to mouse chromosome 4C6. Northern blot analysis showed that expression 3.3-kb mRNA is most abundant in placenta adult kidney expressed at lower levels testis. Whole mount situ hybridization...
ABSTRACT Oxidative injury can occur in the lung through generation of reactive oxygen species (ROS) via redox cycling owing to intentional or accidental ingestion paraquat (PQ), a common herbicide. A wide array phytochemicals has been shown reduce cellular oxidative damage by modulating cytoprotective genes. Quercetin, well‐known flavonoid, reported display effects up‐regulating certain In this context, we investigated effect quercetin on PQ‐induced cytotoxicity alveolar A549 cells,...
In Brief Study Design. Immunohistochemistry and in situ nick end-labeling (TUNEL) were performed rat lumbar intervertebral discs. Objectives. To demonstrate the mechanism of notochordal cell death nucleus pulposus (NP). Summary Background Data. With age, cells gradually disappear NP. We hypothesized that this phenomenon might be related to Fas-mediated apoptosis. Materials Methods. Expressions Fas; Fas ligand (FasL); caspase 3, 8, 9, 10; Ki-67 protein; TUNEL examined 4-week-, 6-month-...
Obesity poses a substantial threat of worldwide epidemic and requires better understanding adipose-tissue biology as well necessitates research into the etiology therapeutic interventions. In this study, Medicarpin (Med), natural pterocarpan, was selected (by screening) small-molecule inducer adipocyte differentiation among 854 candidates by using C3H10T1/2 mesenchymal stem cell; cellular model adipogenesis. Med induced expression brown-adipocyte commitment marker Bmp7 early regulators brown...
Other SectionsAbstractINTRODUCTIONRESULTSDISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTFiguresReferences
We investigated the effects of amitriptyline, a tricyclic antidepressant, on [3H]norepinephrine ([3H]NE) secretion and ion flux in bovine adrenal chromaffin cells. Amitriptyline inhibited [3H]NE induced by 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) 70 mM K+. The half maximal inhibitory concentration (IC50) was 2 μM 9 μM, respectively. also elevation cytosolic calcium ([Ca2+]i) DMPP K+ with IC50 values 1.1 35 rises sodium ([Na+]i) [Ca2+]i Na+ channel activator veratridine were...
Alpha-lipoic acid (LA), a metabolic antioxidant, is natural compound and its biological function has been well studied in various human diseases. The present study was designed to investigate the cytoprotective effect molecular mechanisms of LA paraquat (PQ)-induced oxidative stress injury using BEAS-2B bronchial epithelial cells. co-treatment prevented PQ-induced cell death. also increases total reactive oxygen species (ROS), lactate dehydrogenase (LDH) malondialdehyde (MDA). increased...
Natural pterocarpan Medicarpin (Med) has been shown to have various beneficial biological roles, including inhibition of osteoclastogenesis, stimulation bone regeneration and induction apoptosis. However, the effect Med on lipolysis in adipocytes not reported. Here, we show different mouse elucidate underlying mechanism. We observed that treatment promoted release glycerol media. Differentiated brown adipose tissue cells were treated with Med. RNA-Seq analysis was performed med subsequently...