Hepatitis B virus X protein (HBx) is closely involved in the development of hepatocellular carcinoma, a highly vascularized solid tumor. Here we show that HBx increases transcriptional activity and level hypoxia-inducible factor-1alpha (HIF-1alpha) under both normoxic hypoxic conditions, it also stimulates angiogenesis. directly interacted with bHLH/PAS domain HIF-1alpha but not von Hippel-Lindau (pVHL). decreased binding pVHL to prevented ubiquitin-dependent degradation HIF-1alpha. In...

Angiogenesis is an important mediator of tumor progression. As tumors expand, diffusion distances from the existing vascular supply increases, resulting in hypoxia cancer cells. Sustained expansion a mass requires new blood vessel formation to provide rapidly proliferating cells with adequate oxygen and nutrients. The key regulator hypoxia-induced angiogenesis transcription factor known as hypoxia-inducible (HIF)-1. HIF-1alpha stabilized by reactive species (ROS) enhances expression several...

The sustained expansion of a tumor mass requires new blood vessel formation to provide rapidly proliferating cells with an adequate supply oxygen and nutrients. Hypoxia-inducible factor-1 (HIF-1) plays essential role in angiogenesis growth by regulating the transcription genes response hypoxic stress. This study was designed investigate effects melatonin on angiogenesis, as well mechanism underlying antitumor activities melatonin. In this study, we show that administration inhibits blocks...

p ‐Coumaric acid, a hydroxy derivative of cinnamic has been known to possess antioxidant and anticancer activities. Despite its potential contribution chemopreventive effects, the mechanism by which ‐coumaric acid exerts antiangiogenic actions remains elusive. In this study, we revealed that inhibited sprouting endothelial cells in rat aortic rings tube formation migration cells. We observed could downregulate mRNA expression levels key angiogenic factors vascular growth factor basic...

Hypoxia-inducible factor 1 (HIF-1) plays an essential role in tumor angiogenesis and growth by regulating the transcription of several genes response to hypoxic stress changes factors. This study was designed investigate effects deguelin on angiogenesis, mechanisms underlying antitumor activities deguelin. We show here that orally administered inhibits blocks mice. Deguelin decreased expression HIF-1alpha protein its target genes, such as VEGF, a subset cancer cell lines, including H1299...

Glycyrrhizic acid (GA) is the bioactive compound of licorice and has been used as a herbal medicine because its anti‐viral, anti‐cancer, anti‐inflammatory properties. This study was designed to investigate effects GA on tumor growth, angiogenesis, mechanisms underlying anti‐angiogenic activities GA. We observed that inhibited growth angiogenesis in mice. decreased angiogenic activities, such migration, invasion, tube formation endothelial cells. also demonstrated reduced production reactive...

Midbrain dopamine (mDA) neurons play critical roles in the regulation of voluntary movement and their dysfunction is associated with Parkinson's disease. Pitx3 has been implicated proper development mDA substantia nigra pars compacta, which are selectively lost However, basic mechanisms underlying its role neuron and/or survival poorly understood. Toward this goal, we sought to identify downstream target genes by comparing gene expression profiles wild-type Pitx3-deficient aphakia mice. This...

Cinnamic aldehyde (CA), a key flavor compound in cinnamon essential oil, has been identified as an anti-oxidant, anti-angiogenic, and anti-inflammatory material. Recently, the neuroprotective effects of CA have reported various neurodegenerative disorders, including Parkinson’s disease (PD). In neurons, autophagy is tightly regulated, consequently, dysregulation may induce disorders. present study, we found that selective dopaminergic neuronal death substantia nigra...

Abstract Although peripheral artery disease (PAD) is a major health problem, there have been limited advances in medical therapies. In PAD patients, angiogenesis regarded as promising therapeutic strategy to promote new arterial vessels and improve perfusion of ischemic tissue. Autophagy plays critical role catabolic processes for cell survival under normal stressful conditions fundamental biological roles various cellular functions. the present study, we showed that autophagy endothelial...

Cancer cells undergo uncontrolled proliferation resulting from aberrant activity of various cell-cycle proteins. Therefore, despite recent advances in intensive chemotherapy, it is difficult to cure cancer completely. Recently, regulators became attractive targets therapy. Zingerone, a phenolic compound isolated ginger, nontoxic and inexpensive with varied pharmacological activities. In this study, the therapeutic effect zingerone as an anti-mitotic agent human neuroblastoma was...

Angiogenesis is an essential step for tumor survival and progression, the inhibition of angiogenesis a good strategy therapeutics. In this study, we investigated therapeutic effect zingerone in mouse model. Zingerone suppressed progression angiogenesis. Moreover, found that inhibited angiogenic activities endothelial cells by both direct indirect means. A mechanistic study showed MMP-2 MMP-9 were decreased treatment with zingerone. Interestingly, zingerone-mediated was involved JNK pathway....

Non-thermal atmospheric-pressure plasma has been introduced in various applications such as sterilization, wound healing, blood coagulation, and other biomedical applications.The most attractive application of non-thermal is cancer treatment, where the used to produce reactive oxygen species (ROS) facilitate cell apoptosis.We investigate effects different durations exposure dielectric-barrier discharge (DBD) on colon cells using measurement viability ROS levels, western blot,...

We examined the effects of purified ginseng components, panaxadiol (PD) and panaxatriol (PT), on expression matrix metalloproteinase-9 (MMP-9) in highly metastatic HT1080 human fibrosarcoma cell line. A significant down-regulation MMP-9 by PD PT was detected Northern blot analysis. However, MMP-2 not changed treatment with PT. Quantitative gelatin based zymography confirmed a markedly reduced MMP-9, but To investigate whether level affects invasive capacity cells, we conducted an vitro...