A5026654697- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Reproductive Physiology in Livestock
- Ion Channels and Receptors
- Advanced Glycation End Products research
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Gout, Hyperuricemia, Uric Acid
- Cancer, Hypoxia, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism and Genetic Disorders
- Andrographolide Research and Applications
- Ion channel regulation and function
- Reproductive System and Pregnancy
- Caveolin-1 and cellular processes
- Animal health and immunology
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Cholesterol and Lipid Metabolism
- Calcium signaling and nucleotide metabolism
- Immunodeficiency and Autoimmune Disorders
- Infant Nutrition and Health
- Veterinary medicine and infectious diseases
- Milk Quality and Mastitis in Dairy Cows
Austral University of Chile
2015-2024
Bovine ruminal acidosis is of economic importance as it contributes to reduced milk and meat production. This phenomenon mainly attributed an overload highly fermentable carbohydrate, resulting in increased D (-) lactic acid levels serum plasma. Ruminal correlates with elevated acute phase proteins blood, along neutrophil activation infiltration into various tissues leading laminitis aseptic polysynovitis. Previous studies bovine neutrophils indicated that decreased expression L-selectin...
Non-esterified fatty acids (NEFAs) such as oleic acid (OA) and linoleic (LA) are associated with a higher incidence of infectious diseases metritis mastitis during the bovine peripartum. Fatty can induce an increase in release ATP, changes expression levels purinergic receptors polymorphonuclears (PMN) peripartum have also been reported. PMN respond to inflammatory processes production ROS, proteolytic bactericidal proteins, formation neutrophil extracellular traps (NETs). NETs is known...
N-Formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF) induce similar intracellular signalling profiles; but only fMLP induces interleukin-8 (IL-8) release nicotinamide adenine dinucleotide phosphate reduced (NADPH) oxidase activity in neutrophils. Because the role of ROS on IL-8 neutrophils is until now controversial, we assessed if NADPH involved secretions PI3K/Akt, MAPK, NF-κB pathways induced by fMLP. Neutrophils were obtained from healthy volunteers. was...
Fatty acids have been recognized as regulators of immune function in addition to their known metabolic role. Long-chain fatty bind free acid receptor (FFAR)-1/GPR40, which is expressed on bovine neutrophils, and increase responses such granule release gene expression. In this study, we investigated the molecular mechanisms governing up-regulation cyclooxygenase-2 (COX-2) IL-8, well matrix metalloproteinase (MMP)-9 FFAR1/GPR40 agonist-stimulated neutrophils. Our results showed that natural...
Abstract Lameness is a common condition in dairy cattle caused by infectious or noninfectious agents. Joint lesions are the second most cause of lameness and can be diagnosed association with presentation digit injuries. Fibroblast-like synoviocyte (FLS) predominant cells synovia play key role pathophysiology joint diseases, thus increasing expression proinflammatory mediators. Tumor necrosis factor-alpha (TNF-α) potent cytokine involved cyclooxygenase 2 (COX-2) FLS. Previously, TNF-α was...
Abstract Neutrophil’s responses to G protein-coupled chemoattractants are highly dependent on store-operated calcium (Ca2+) entry (SOCE). Platelet-activating factor (PAF), a primary chemoattractant, simultaneously increases cytosolic-free Ca2+, intracellular pH (pHi), ERK1/2, and Akt/protein kinase B (PKB) phosphorylation. In this study, we looked at the efficacy of several putative SOCE inhibitors whether mediates alkalinization, Akt/PKB phosphorylation in bovine neutrophils. We...
Reactive oxygen species (ROS) production is one of the main mechanisms used to kill microbes during innate immune response.D-lactic acid, which augmented acute ruminal acidosis, reduces platelet activating factor (PAF)-induced ROS and L-selectin shedding in bovine neutrophils vitro.This study was conducted investigate whether acidosis induced by oligofructose overload heifers interferes with blood neutrophils.Blood plasma were obtained jugular venipuncture, while samples collected using...
Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA the result increased intake highly fermentable carbohydrates that frequently occurs in dairy subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are synovial fluid, including cytokines, prostaglandin E2 (PGE2), metalloproteinases, neutrophil extracellular traps (NETs). The aim this study was identify...
Acute ruminal acidosis (ARA) is caused by the excessive intake of highly fermentable carbohydrates, followed massive production D-lactate and appearance neutrophilic aseptic polysynovitis. Bovines with ARA develop different lesions, such as ruminitis, polioencephalomalacia (calves), liver abscess lameness. Lameness in cattle closely associated presence laminitis However, despite decades research bovine lameness consequence acidosis, aetiology pathogenesis remain unclear. Fibroblast-like...
Abstract Elevated plasma concentrations of the ketone body β-hydroxybutyrate (BHB), an endogenous agonist hydroxycarboxylic acid receptor 2 (HCA2), is associated with increased incidence inflammatory diseases during lactation in dairy cows. In early stages this pathology, increase neutrophil recruitment observed; however, role BHB remains elusive. This study characterized effect and synthetic agonists HCA2 on bovine chemotaxis signaling pathways involved process. We demonstrated that...
Acute ruminal acidosis (ARA) occurs after an excessive intake of rapidly fermentable carbohydrates and is characterized by the overproduction D-lactate in rumen that reaches bloodstream. Lameness presentation, one primary consequences ARA cattle, associated with occurrence laminitis aseptic polysynovitis. Fibroblast-like synoviocytes (FLS) are predominant cells synovia play a key role pathophysiology joint diseases, thus increasing chances release pro-inflammatory cytokines. Increased levels...
Nonesterified fatty acids (NEFAs) are involved in proinflammatory processes cattle, including the increased expression of adhesion molecules endothelial cells. However, mechanisms underlying these effects still unknown. The aim this study was to assess NEFAs on intracellular calcium (Ca2+ i) influx, nitric oxide production, and ICAM-1 IL-8 primary bovine umbilical vein cells (BUVECs). Myristic (MA), palmitic (PA), stearic (SA), oleic (OA) linoleic acid (LA) rapidly Ca2+ i. response all...