A5057143697- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Reproductive Physiology in Livestock
- Cell Adhesion Molecules Research
- Andrographolide Research and Applications
- Gout, Hyperuricemia, Uric Acid
- Ion Channels and Receptors
- Nitric Oxide and Endothelin Effects
- Phytochemicals and Antioxidant Activities
- Fatty Acid Research and Health
- Receptor Mechanisms and Signaling
- Signaling Pathways in Disease
- Reproductive System and Pregnancy
- Inflammation biomarkers and pathways
- Tea Polyphenols and Effects
- Lung Cancer Treatments and Mutations
- Genomics, phytochemicals, and oxidative stress
- Protein Kinase Regulation and GTPase Signaling
- Ion channel regulation and function
- Animal health and immunology
- Advanced Glycation End Products research
- Caveolin-1 and cellular processes
- Tannin, Tannase and Anticancer Activities
- Lymphoma Diagnosis and Treatment
Austral University of Chile
2016-2025
University of Córdoba
2014
Valparaiso University
2012
Consejo Superior de Investigaciones Científicas
2011-2012
Instituto de Ciencia y Tecnología de Alimentos y Nutrición
2012
Universidad Nacional de Córdoba
2009
Universidad de Burgos
2004
Charité - Universitätsmedizin Berlin
2004
City Of Hope National Medical Center
2004
City of Hope
2004
Eimeria bovis is an important coccidian parasite that causes high economic losses in the cattle industry. We recently showed polymorphonuclear neutrophils (PMN) react upon E. sporozoite exposure by neutrophil extracellular trap (NET) formation. focused here on molecular mechanisms are involved this process. The encounter led to enhanced surface expression of CD11b suggesting a potential role receptor bovis-mediated NETosis. Antibody-mediated blockage confirmed assumption and significantly...
1. Andrographolide, the major active component from Andrographis paniculata, has shown to possess anti-inflammatory activity. Andrographolide inhibits expression of several proinflammatory proteins that exhibit a nuclear factor kappa B (NF-kappaB) binding site in their gene. 2. In present study, we analyzed effect andrographolide on activation NF-kappaB induced by platelet-activating (PAF) and N-formyl-methionyl-leucyl-phenylalanine (fMLP) HL-60 cells differentiated neutrophils. 3. PAF (100...
Bovine ruminal acidosis is of economic importance as it contributes to reduced milk and meat production. This phenomenon mainly attributed an overload highly fermentable carbohydrate, resulting in increased D (-) lactic acid levels serum plasma. Ruminal correlates with elevated acute phase proteins blood, along neutrophil activation infiltration into various tissues leading laminitis aseptic polysynovitis. Previous studies bovine neutrophils indicated that decreased expression L-selectin...
Anthocyanins are pigments with antihyperglycemic properties, and they potential candidates for developing functional foods the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism these beneficial effects anthocyanins are, however, hard to explain, given their very low bioavailability due poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect anthocyanidin delphinidin over glucose show direct on...
Non-esterified fatty acids (NEFAs) such as oleic acid (OA) and linoleic (LA) are associated with a higher incidence of infectious diseases metritis mastitis during the bovine peripartum. Fatty can induce an increase in release ATP, changes expression levels purinergic receptors polymorphonuclears (PMN) peripartum have also been reported. PMN respond to inflammatory processes production ROS, proteolytic bactericidal proteins, formation neutrophil extracellular traps (NETs). NETs is known...
N-Formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF) induce similar intracellular signalling profiles; but only fMLP induces interleukin-8 (IL-8) release nicotinamide adenine dinucleotide phosphate reduced (NADPH) oxidase activity in neutrophils. Because the role of ROS on IL-8 neutrophils is until now controversial, we assessed if NADPH involved secretions PI3K/Akt, MAPK, NF-κB pathways induced by fMLP. Neutrophils were obtained from healthy volunteers. was...
Fatty acids have been recognized as regulators of immune function in addition to their known metabolic role. Long-chain fatty bind free acid receptor (FFAR)-1/GPR40, which is expressed on bovine neutrophils, and increase responses such granule release gene expression. In this study, we investigated the molecular mechanisms governing up-regulation cyclooxygenase-2 (COX-2) IL-8, well matrix metalloproteinase (MMP)-9 FFAR1/GPR40 agonist-stimulated neutrophils. Our results showed that natural...
Abstract Lameness is a common condition in dairy cattle caused by infectious or noninfectious agents. Joint lesions are the second most cause of lameness and can be diagnosed association with presentation digit injuries. Fibroblast-like synoviocyte (FLS) predominant cells synovia play key role pathophysiology joint diseases, thus increasing expression proinflammatory mediators. Tumor necrosis factor-alpha (TNF-α) potent cytokine involved cyclooxygenase 2 (COX-2) FLS. Previously, TNF-α was...
Abstract Neutrophil’s responses to G protein-coupled chemoattractants are highly dependent on store-operated calcium (Ca2+) entry (SOCE). Platelet-activating factor (PAF), a primary chemoattractant, simultaneously increases cytosolic-free Ca2+, intracellular pH (pHi), ERK1/2, and Akt/protein kinase B (PKB) phosphorylation. In this study, we looked at the efficacy of several putative SOCE inhibitors whether mediates alkalinization, Akt/PKB phosphorylation in bovine neutrophils. We...
Long chain fatty acids (LCFAs), which are ligands for the G-protein coupled receptor FFAR1 (GPR40), increased in cow plasma after parturition, a period they highly susceptible to infectious diseases. This study identified and analyzed functional role of bovine neutrophils, first line host defense against agents. We cloned putative from neutrophils sequence construct homology model. Our results revealed that shares 84% identity with human 31% FFAR3/GPR41. Therefore, we constructed model using...
Kinins are pro-inflammatory peptides that mimic the cardinal features of inflammation. We examined concept expression levels endothelial intercellular adhesion molecule-1 (ICAM-1) and neutrophil integrins Mac-1 LFA-1 modulated by kinin B 1 receptor (B R) agonist, Lys-des[Arg 9 ]bradykinin (LDBK). Stimulation cells with LDBK increased ICAM-1 mRNA transcripts/protein, also E-selectin platelet molecule-1. in a magnitude comparable produced TNF-α. This stimulatory effect was reduced when cells,...
Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA the result increased intake highly fermentable carbohydrates that frequently occurs in dairy subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are synovial fluid, including cytokines, prostaglandin E2 (PGE2), metalloproteinases, neutrophil extracellular traps (NETs). The aim this study was identify...