A5002817936- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Fatty Acid Research and Health
- Veterinary medicine and infectious diseases
- Immune cells in cancer
- Neonatal Respiratory Health Research
- Pregnancy-related medical research
- Reproductive Physiology in Livestock
- Diabetes Treatment and Management
- Effects of Environmental Stressors on Livestock
- Reproductive System and Pregnancy
- NF-κB Signaling Pathways
- Birth, Development, and Health
- Andrographolide Research and Applications
- Biochemical Analysis and Sensing Techniques
- Lipid metabolism and disorders
- Immune Cell Function and Interaction
- Parasitic Diseases Research and Treatment
- Virus-based gene therapy research
- Ginseng Biological Effects and Applications
- Microbial infections and disease research
- Ruminant Nutrition and Digestive Physiology
Austral University of Chile
2015-2024
Anthocyanins are pigments with antihyperglycemic properties, and they potential candidates for developing functional foods the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism these beneficial effects anthocyanins are, however, hard to explain, given their very low bioavailability due poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect anthocyanidin delphinidin over glucose show direct on...
N-Formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF) induce similar intracellular signalling profiles; but only fMLP induces interleukin-8 (IL-8) release nicotinamide adenine dinucleotide phosphate reduced (NADPH) oxidase activity in neutrophils. Because the role of ROS on IL-8 neutrophils is until now controversial, we assessed if NADPH involved secretions PI3K/Akt, MAPK, NF-κB pathways induced by fMLP. Neutrophils were obtained from healthy volunteers. was...
Fatty acids have been recognized as regulators of immune function in addition to their known metabolic role. Long-chain fatty bind free acid receptor (FFAR)-1/GPR40, which is expressed on bovine neutrophils, and increase responses such granule release gene expression. In this study, we investigated the molecular mechanisms governing up-regulation cyclooxygenase-2 (COX-2) IL-8, well matrix metalloproteinase (MMP)-9 FFAR1/GPR40 agonist-stimulated neutrophils. Our results showed that natural...
Abstract Lameness is a common condition in dairy cattle caused by infectious or noninfectious agents. Joint lesions are the second most cause of lameness and can be diagnosed association with presentation digit injuries. Fibroblast-like synoviocyte (FLS) predominant cells synovia play key role pathophysiology joint diseases, thus increasing expression proinflammatory mediators. Tumor necrosis factor-alpha (TNF-α) potent cytokine involved cyclooxygenase 2 (COX-2) FLS. Previously, TNF-α was...
Abstract Acute ruminal acidosis (ARA) is the result of increased intake highly fermentable carbohydrates, which frequently occurs in dairy cattle and associated with aseptic polysynovitis. To characterise metabolic changes joints animals ARA, we performed an untargeted gas chromatography–mass spectrometry (GC-MS)-based metabolomic analysis synovial fluid. Seven heifers were challenged intraruminal oligofructose overload (13 g/kg body weight [BW]) dissolved water. Synovial fluid samples...
Acute ruminal acidosis (ARA) is caused by the excessive intake of highly fermentable carbohydrates, followed massive production D-lactate and appearance neutrophilic aseptic polysynovitis. Bovines with ARA develop different lesions, such as ruminitis, polioencephalomalacia (calves), liver abscess lameness. Lameness in cattle closely associated presence laminitis However, despite decades research bovine lameness consequence acidosis, aetiology pathogenesis remain unclear. Fibroblast-like...
Acute ruminal acidosis (ARA) occurs after an excessive intake of rapidly fermentable carbohydrates and is characterized by the overproduction D-lactate in rumen that reaches bloodstream. Lameness presentation, one primary consequences ARA cattle, associated with occurrence laminitis aseptic polysynovitis. Fibroblast-like synoviocytes (FLS) are predominant cells synovia play a key role pathophysiology joint diseases, thus increasing chances release pro-inflammatory cytokines. Increased levels...
Interleukin-3 (IL-3) is a well-characterized growth factor in hematopoietic cells, but it also expressed other cell types with poorly described functions. Many studies have provided evidence that IL-3 plays an important role survival. We previously shown able to increase glucose uptake HEK293 suggesting this requires sustained metabolism promote In study, we demonstrate contributes survival under oxidative stress, prominent feature the pathophysiology of cancer, diabetes, and...
During acute ruminal acidosis, the manifestation of aseptic polysynovitis and lameness in cattle has been observed. Evidence suggests that joint inflammation can be attributed to metabolic alterations induced by D-lactate fibroblast-like synoviocytes (FLSs). We aimed investigate whether andrographolide could mitigate bovine (bFLSs). To assess this, bFLSs were cultured presence or absence andrographolide. evaluated its potential interference with expression proinflammatory cytokines, COX-2,...
Fasciola hepatica is a parasitic trematode that causes fasciolosis in sheep, provoking decrease their reproductive capacity, weight gain, meat and milk production, wool quality. In the pathogenesis of F. hepatica, penetration migration stages through liver provoke intense inflammatory immune responses tissue damage. The aim this study was to investigate cytotoxic effects Fascila hepatica-induced ovine NETs exposed hepatocytes vitro, analyze whether antigens (FhAg) trigger release under...
The bovine endometrium has an important defensive role in the postpartum period that acts when inflammatory process associated with tissue damage or infection by bacteria is produced. Endometrial cells release cytokines and chemokines recruit cells, which danger-associated molecular patterns (DAMPs), such as adenosine triphosphate (ATP), initiate regulate response. However, of ATP endometrial unclear. aim this study was to determine effect on interleukin-8 (IL-8) release, intracellular...
Results: Anti-CD8α treatment depleted CD8+ T cells in blood efficiently but not placenta.Placental CD3+CD8+ cell frequency was increased by LPS and decreased DEP.Placental that survive depleting mAb have of effector subset showed the lower capability IFN-γ production following maternal acute inflammation.Expression CD44 on DEP.Conclusions: We demonstrated can altered phenotype function, including cytotoxicity, trafficking, memory, cytokine production, dependent conditions which they are...