A5040618529- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Colorectal Cancer Treatments and Studies
- CRISPR and Genetic Engineering
- Helicobacter pylori-related gastroenterology studies
- Breast Cancer Treatment Studies
- Cancer, Lipids, and Metabolism
- Eosinophilic Esophagitis
- Thyroid Disorders and Treatments
- AI in cancer detection
- RNA and protein synthesis mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Estrogen and related hormone effects
- Thyroid Cancer Diagnosis and Treatment
- Digital Imaging for Blood Diseases
- Colorectal Cancer Screening and Detection
- Cancer-related Molecular Pathways
- Cancer Risks and Factors
- Sarcoma Diagnosis and Treatment
- Gene expression and cancer classification
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- Whipple's Disease and Interleukins
Olabisi Onabanjo University
2015-2025
Olabisi Onabanjo University Teaching Hospital
2016-2023
University of Nottingham
2016-2022
Queen's Medical Centre
2018-2019
University College Hospital, Ibadan
2012-2015
University of Ibadan
2014
Recent advances in whole-slide imaging (WSI) technology have led to the development of a myriad computer vision and artificial intelligence-based diagnostic, prognostic, predictive algorithms. Computational Pathology (CPath) offers an integrated solution utilise information embedded pathology WSIs beyond what can be obtained through visual assessment. For automated analysis validation machine learning (ML) models, annotations at slide, tissue, cellular levels are required. The annotation...
Abstract Objectives: Prostate cancer (PCa) is the most common malignancy in men. Geography and environmental factors have been associated with varying incidence mortalities different groups. Vitamin D has antiproliferative effect on PCa cells, its mediated through vitamin receptor (VDR). This study reported correlation of VDR expression some clinicopathological biological features among a cohort patients PCa. Methods: Genomic data 497 reposited The Cancer Genome Atlas were retrieved using...
Introduction: Sickle Cell Disease (SCD) has a high mortality rate in the environment where we practice. There is lack of contemporal autopsy studies describing causes death among SCD patients at our centre.
Background: The prevalence of goitrous swelling has reduced in Nigeria since the introduction salt iodisation programme. Thyroid disorders are second most common endocrine disorder after diabetes mellitus worldwide. They present to general outpatient, medical and surgical clinics accompanied by great anxiety poor health-related quality life.Objectives: study aimed determine describe spectrum thyroid seen at Olabisi Onabanjo University Teaching Hospital over a 10-year period.Materials...
Objective . The osteoporosis in thyroid disorder has the lowest report especially sub-Saharan Africa. This study aims to determine prevalence, predictive factors, and characteristics of hyperthyroid patients. Method Forty (40) patients healthy controls ages 21–50 years were recruited this study. Questionnaires administered capture bio- clinical data. Biochemical tests included blood, functions, intact parathyroid hormone, corrected calcium, 25-hydroxyvitamin D tests. Bone mineral density...
Whilst risk prediction for individual prostate cancer (PCa) cases is of a high priority, the current stratification indices PCa management have severe limitations. This study aimed to identify gene copy number alterations (CNAs) with prognostic values and determine if any combination CNAs could potentials. Clinical genomic data 500 from Cancer Genome Atlas stable were retrieved Genomic Data Commons cBioPortal databases. The CNA statuses total 52 genetic markers, including 21 novel markers 31...
Background The clinicopathological significance of KRAS alterations in clinical prostate cancer (PCa) has yet to be comprehensively studied, and the classic somatic mutations are rare PCa. Methods clinico-genomic data two PCa cohorts were retrieved from genome databases. expression-based gene enrichment for cell proliferation, apoptosis, epithelial-mesenchymal transition /invasion programmes, RAS activation, MAPK PI3K signalling sought using analyses, validated with clinicopathologically...
We sought to use PCR followed by high-resolution melting analysis develop a single closed-tube screening panel screen for Lynch syndrome. This comprises tests microsatellite instability (MSI), MLH1 methylation promoter and BRAF mutation.For MSI testing, five mononucleotide markers (BAT25, BAT26, BCAT25, MYB, EWSR1) were developed. In addition, primers designed interrogate Region C of the (using bisulphite-modified DNA) test mutations in codon 600 BRAF. Two separate cohorts from Nottingham...
Aim: The most recent study on the clinicopathological features of gastric carcinoma from University College Hospital (UCH), Ibadan, was done in 2000. aim this is to update knowledge diagnosed Pathology Department UCH Ibadan between 2000 and 2011. Materials Methods: This a 12-year retrospective review clinical demographic data histopathological cancers at UCH. chi square test, Fisher's exact t-independent test were used as applicable statistical analyses. Results: A total 117 cases...
Disparities in DNA template quantity across sample wells is a common occurrence PCR experiments and frequent cause of bogus results research diagnostics. High Resolution Melt (HRM) analysis, robust genotyping technique, was shown to be highly reproducible irrespective the reaction volume concentration used for PCR. The aim this study determine if HRM can maintain its reproducibility/reliability when varying amounts starting templates are utilized upstream PCR.Serial dilutions differentially...
Aim: To demonstrate that MSI-WES is an accurate testing method for microsatellite instability (MSI). Materials & methods: Microsatellite-based indels were counted in the variant call-formatted whole exome sequencing (WES) data of 441 gastric cancer cases using Unix-based algorithms, and counts expressed as a fraction genome sequenced to obtain next-generation sequencing-based MSI indices. Results: The indices showed near-perfect concordance with PCR-based status, moderate good correlations...
High-resolution melt analysis (HRMA) is a cheap and reliable post-polymerase chain reaction (PCR) cancer mutation screening technique, which fast gaining clinical relevance. The HRMA capabilities of the LightScanner (Idaho Technology) have been severally studied. However, ABI 7500 HRM has not tested against purpose-built instrument such as LightScanner. DNA from formalin-fixed, paraffin-embedded gastric cancer, colorectal normal tissue well cell lines were amplified at exons 2, 3, 4 KRAS, 11...
Currently, short DNA segments of sub-100 bp can be sequenced either directly by next-generation sequencing and pyrosequencing, which are expensive, or indirectly, via Sanger combined with the cumbersome failure-prone plasmid cloning. To circumvent these issues, we have generated a novel sequencing-purposed PCR assay using long-tailed primers (squirrel primers) to sequence genomic amplicons. Squirrel primers, 40-65 nt in length, were used amplify 51-93 long sequences KRAS exons 2 3, BRAF exon...
Gene dosage can have a major impact on cell biology, although, hitherto, it has been difficult to study using in vitro models. We sought refine and accelerate the development of 'gene dosage' models through CRISPR/Cas9 (a gene editing technology) for sequential knockout alleles.
Prostaglandin-endoperoxide synthase-2 (ptgs2), otherwise called Cyclooxygenase 2, is overexpressed in colorectal carcinoma (CRC) compared to normal tissues. However, the impact of differential expression among ptgs2-positive tumours on CRC prognosis has not been well investigated. By sub-stratifying positive tumour expression, this study determined its potential influence patients' outcomes. The Cancer Genome Atlas database was explored determine cases with RNA-Sequence (RNA-Seq) transcript...
Currently, risk stratification is the most difficult problem in prostate cancer (PCa) management. Gleason grading cannot adequately predict progression. This study aimed to identify chromosome-specific segment size alterations that could aid and metastasis using a retrospective cohort-study strategy. A binary logistic regression model was generated 16 segments with (deletions amplifications) showed associations disease stage (primary versus metastatic). The trained MSKCC PIK3R1 PCa cohort (n...
Ovarian cancer is the leading cause of death from all gynaecological malignancies. Only few biomarkers epithelial ovarian (EOC) prognosis have been studied so far among Nigerian patients.