A5042509710- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- interferon and immune responses
- Helicobacter pylori-related gastroenterology studies
- Histone Deacetylase Inhibitors Research
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Galectins and Cancer Biology
- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Influenza Virus Research Studies
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Cancer-related gene regulation
- Immune cells in cancer
- Digestive system and related health
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
Augusta University
2013-2025
Augusta University Health
2016-2025
Georgia Regents Medical Center
2013-2016
Cancer Research Center
2014
Emory University
2009-2011
Emory National Primate Research Center
2008-2011
Emory Healthcare
2008-2010
University of Illinois Chicago
2005-2009
University of Illinois Urbana-Champaign
2005-2008
University of Chicago Medical Center
2008
Dendritic cells (DCs) play a vital role in initiating robust immunity against pathogens as well maintaining immunological tolerance to self antigens. However, the intracellular signaling networks that program DCs become tolerogenic remain unknown. We report here Wnt-beta-catenin intestinal dendritic regulates balance between inflammatory versus regulatory responses gut. beta-catenin was required for expression of anti-inflammatory mediators such retinoic acid-metabolizing enzymes,...
Influenza A virus is being extensively studied because of its major impact on human and animal health. However, the dynamics influenza infection cell types infected in vivo are poorly understood. These characteristics challenging to determine, partly there no efficient replication-competent expressing an easily traceable reporter gene. Here, we report generation a recombinant carrying GFP gene NS segment (NS1-GFP virus). Although attenuated when compared with wild-type virus, NS1-GFP...
Although several subsets of intestinal APCs have been described, there has no systematic evaluation their phenotypes, functions, and regional localization to date. In this article, we used 10-color flow cytometry define the major APC in small large intestine lamina propria. Lamina propria could be subdivided into CD11c(+)CD11b(-), CD11c(+)CD11b(+), CD11c(dull)CD11b(+) subsets. CD11c(+)CD11b(-) cells were largely CD103(+)F4/80(-) dendritic (DCs), whereas CD11c(+)CD11b(+) subset comprised...
Mammary stem/progenitor cells (MaSCs) maintain self-renewal of the mammary epithelium during puberty and pregnancy. DNA methylation provides a potential epigenetic mechanism for maintaining cellular memory self-renewal. Although methyltransferases (DNMTs) are dispensable embryonic stem cell maintenance, their role in MaSCs cancer (CSCs) constantly replenishing is unclear. Here we show that DNMT1 indispensable MaSC maintenance. Furthermore, find expression elevated tumours, gland-specific...
Recently, impressive technical advancements have been made in the isolation and validation of mammary stem cells cancer (CSC), but signaling pathways that regulate cell self-renewal are largely unknown. Furthermore, CSCs believed to contribute chemo- radioresistance. In this study, we used MMTV-Neu-Tg mouse tumor model identify potential new strategies for eliminating CSCs. We found both luminal progenitor basal susceptible genetic epigenetic modifications, which facilitate oncogenic...
Abstract At mucosal sites such as the intestine, immune system launches robust immunity against invading pathogens while maintaining a state of tolerance to commensal flora and ingested food Ags. The molecular mechanisms underlying this phenomenon remain poorly understood. In study, we report that signaling by GPR81, receptor for lactate, in colonic dendritic cells macrophages plays an important role suppressing inflammation restoring homeostasis. Genetic deletion GPR81 mice led increased...
Breakdown in immunological tolerance to self-Ags or uncontrolled inflammation results autoimmune disorders. Dendritic cells (DCs) play an important role regulating the balance between inflammatory and regulatory responses periphery. However, factors tissue microenvironment signaling networks critical for programming DCs control chronic promote are unknown. In this study, we show that wnt ligand-mediated activation of β-catenin is promoting limiting neuroinflammation. DC-specific deletion key...
Tumors actively suppress antitumor immunity, creating formidable barriers to successful cancer immunotherapy. The molecular mechanisms underlying tumor-induced immune tolerance are largely unknown. In the present study, we show that dendritic cells (DC) in tumor microenvironment acquire ability metabolize vitamin A produce retinoic acid (RA), which drives regulatory T-cell responses and tolerance. Tolerogenic were dependent on induction of A-metabolizing enzymes via β-catenin/T-cell factor...
Organ cross talk exists in many diseases of the human and animal models diseases. A recent study demonstrated that inflammatory mediators can cause acute kidney injury neutrophil infiltration a mouse model dextran sodium sulfate (DSS)-colitis. However, chemokines their receptors may mediate distant organ effects colitis are unknown. We hypothesized keratinocyte chemoattractant (KC)/IL-8 receptor chemokine (C-X-C motif) ligand 2 (CXCL2) mediates DSS-colitis-induced injury. Consistent with our...
Abstract Dendritic cells (DCs) sense microbes via multiple innate receptors. Signals from different receptors are coordinated and integrated by DCs to generate specific adaptive immune responses against pathogens. Previously, we have shown that two pathogen recognition receptors, TLR2 dectin-1, which recognize the same microbial stimulus (zymosan) on DCs, induce mutually antagonistic regulatory or inflammatory responses, respectively. How diametric signals these in regulate incite immunity...
Abstract Dietary lipids and their metabolites activate members of the peroxisome proliferative–activated receptor (PPAR) family transcription factors are critical for colonic health. The PPARα isoform plays a vital role in regulating inflammation various disease settings, but its intestinal inflammation, commensal homeostasis, mucosal immunity gut unclear. In this study, we demonstrate that pathway innate immune cells orchestrates homeostasis by expression IL-22 antimicrobial peptides...
Retinoic acid inducible gene-I (RIG-I) is an innate RNA sensor that recognizes the influenza A virus (IAV) genome and activates antiviral host responses. Here, we demonstrate RIG-I signaling plays a crucial role in restricting IAV tropism regulating immune Mice deficient RIG-I-MAVS pathway show defects migratory dendritic cell (DC) activation, viral antigen presentation, priming of CD8+ CD4+ T responses during infection. These result decreased frequency polyfunctional effector cells lowered...
Macroautophagy/autophagy contributes to maladaptive kidney repair by inducing pro-fibrotic factors such as FGF2 (fibroblast growth factor 2), but the underlying mechanism remains elusive. Here, we show that EGR1 (early response 1) was induced in injured proximal tubules after ischemic acute injury (AKI) and this induction suppressed autophagy deficiency inducible, renal tubule-specific atg7 (autophagy related 7) knockout (iRT-atg7 KO) mice. In cultured tubular cells, TGFB1 (transforming beta...