A5015591780- Influenza Virus Research Studies
- Immune Response and Inflammation
- interferon and immune responses
- Reproductive tract infections research
- Immune Cell Function and Interaction
- Syphilis Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Cell Adhesion Molecules Research
- Animal Virus Infections Studies
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Vitamin C and Antioxidants Research
- Endometriosis Research and Treatment
- Mycobacterium research and diagnosis
- Insect Resistance and Genetics
- Animal Disease Management and Epidemiology
- Female Genital Mutilation/Cutting Issues
- Sex work and related issues
National Center for Immunization and Respiratory Diseases
2014-2024
Centers for Disease Control and Prevention
2015-2024
Zhenjiang City Fourth People's Hospital
2015-2022
Jiangsu University
2012-2022
The Fourth People's Hospital
2015-2022
Oak Ridge Associated Universities
2021
Oak Ridge Institute for Science and Education
2021
National Center for HIV/AIDS Viral Hepatitis STD and TB Prevention
2021
People's Hospital of Bishan District
2020
Emory and Henry College
2010
Human DCs (dendritic cells) express surface CD83 upon activation. Comparing the induction of with upregulation CD40, CD80 and CD86 during LPS (lipopolysaccharide)-induced DC maturation showed that occurred more rapidly. Despite lack on immature DCs, it was detected in these cells by Western blotting flow cytometry. Indirect immunofluorescence revealed inside perinuclear regions. absent monocytes macrophages, but found to be rapidly surface-expressed LPS-induced Whereas expression activated...
Abstract Tunneling nanotubes (TNTs) represent a novel route of intercellular communication. While previous work has shown that TNTs facilitate the exchange viral or prion proteins from infected to naïve cells, it is not clear whether genome also transferred via this mechanism and further, transfer can result in productive replication infectious agents recipient cell. Here we present evidence lung epithelial cells are connected by TNTs, spite presence neutralizing antibodies an antiviral...
Protein energy malnutrition (PEM), a common cause of secondary immune deficiency in children, is associated with an increased risk infections. Very few studies have addressed the relevance PEM as factor for influenza.
Apoptosis induction is an antiviral host response, however, influenza A virus (IAV) infection promotes cell death. The nucleoprotein (NP) of IAV known to contribute viral pathogenesis, but its role in virus-induced death was hitherto unknown. We observed that NP contributes induced and heterologous expression alone can induce apoptosis human airway epithelial cells. apoptotic effect significant when compared with other proapoptotic proteins IAV. by executed through the intrinsic pathway....
ABSTRACT Pattern recognition receptors (PRR) sense certain molecular patterns uniquely expressed by pathogens. Retinoic-acid-inducible gene I (RIG-I) is a cytosolic PRR that senses viral nucleic acids and induces innate immune activation secretion of type interferons (IFNs). Here, using influenza vaccine antigens, we investigated the consequences activating RIG-I pathway for antigen-specific adaptive responses. We found mice immunized with antigens coadministered 5′ppp-double-stranded RNA...
Toll-like receptors (TLRs) are activated by microbial structures. To investigate the mechanisms of TLR activation, 10 human TLRs were expressed as chimeras with integrin alphav and beta5 subunits. Co-expression alphav-TLR beta5-TLR in 293T cells generated homodimers, but only TLR4/4 could effectively activate NF-kappaB. TLR4 monomers also NF-kappaB it was enhanced upon homodimerization. The homodimers showed differential surface/intracellular expression. In heterodimers, TLR2/1 TLR2/6 potent...
ABSTRACT Recognition of pathogen-associated molecular patterns by pattern recognition receptors the innate immune system is crucial for initiation and adaptive responses immunological memory. We investigated role TLR7 in induction immunity long-term memory following influenza virus infection vaccination C57BL/6 mice. During with A/PR8/34 virus, absence either or MyD88 leads to reduced virus-specific antibodies serum antibody-secreting cells their secondary lymphoid organs, particularly bone...
Abstract Myeloid dendritic cells (mDCs) have long been thought to function as classical APCs for T cell responses. However, we demonstrate that influenza viruses induce rapid differentiation of human monocytes into mDCs. Unlike the classic mDCs, virus-induced mDCs failed upregulate DC maturation markers and were unable allogeneic lymphoproliferation. Virus-induced secreted little, if any, proinflammatory cytokines; however, they a substantial amount chemoattractants (MCP-1 IP-10)....
The NLR protein, NLRC5 is an important regulator of MHC class I gene expression, however, the role in other innate immune responses less well defined. In present study, we report that binds RIG-I and this interaction critical for robust antiviral against influenza virus. Overexpression human lung epithelial cell line, A549, normal bronchial cells resulted impaired replication virus A/Puerto Rico/8/34 (PR8) enhanced IFN-β expression. Influenza leads to induction drives expression host cells....
Abstract Background. Influenza disproportionately impacts older adults while current vaccines have reduced effectiveness in the population. Methods. We conducted a comprehensive evaluation of cellular and humoral immune responses aged 50 years to 2008–2009 seasonal trivalent inactivated influenza vaccine assessed factors influencing response. Results. Vaccination increased hemagglutination inhibition neutralizing antibody; however, 66.3% subjects did not reach titers ≥ 40 for H1N1, compared...
Abstract Background RIV4 and cell-culture based inactivated influenza vaccine (ccIIV4) have not been compared to egg-based IIV4 in healthcare personnel, a population with frequent vaccination that may blunt immune responses over time. We conducted randomized trial among personnel (HCP) aged 18–64 years compare humoral ccIIV4 IIV4. Methods During the 2018–2019 season, participants were receive ccIIV4, RIV4, or had serum samples collected prevaccination, 1 6 months postvaccination. Serum...
Syphilis is a sexually transmitted, disseminated acute and chronic infection caused by the bacterial pathogen Treponema pallidum subspecies . Primary syphilis typically presents as single or multiple mucocutaneous lesions and, if left untreated, can progress through stages with various clinical manifestations.
Abstract Background Sexually transmitted infections (STIs) such as syphilis and HIV remain to be a significant public health issue worldwide. Dual rapid point-of-care tests (POCTs) have shown promise for detecting antibodies but not been fully evaluated in the field. Our study supported WHO ProSPeRo on Transmitted Infection Point-of-Care Testing (STI POCT) by providing external quality assessment (EQA) testing reference laboratories their associated clinical sites seven countries. Methods...
Dendritic cells (DCs) accumulate in atherosclerotic lesions but their characteristics and role atherogenesis are poorly understood. C1q, an element of the first component complement, is expressed by interdigitating dendritic follicular spleen. It has been suggested that C1q involved capturing immune complexes lymphoid tissue. Immune also detected lesions. The present study investigated whether DCs arterial wall. Because accumulating within might originate from monocytes infiltrate intima...
The aim of this study was to examine the interaction Notch/Notch ligand with Th17/Treg, cytokines IL-35 and IL-17 in cases preeclampsia (PE). Methods. Peripheral blood obtained from 42 PE patients 22 health pregnant women. mRNA expressions ligand, Treg transcription factor FoxP3 Th17 ROR γ t, EBI3 P35 (IL-35 two subunits), were determined by qPCR. serum levels measured ELISA. Results. It observed that Foxp3, EBI3, lower compared normal pregnancy, whereas t expression significantly increased....
The objective of the present study was to investigate role blood glucose, lipid metabolism, body mass index (BMI), C-reactive protein (CRP) as well an interleukin (IL)-17/IL-35 imbalance in pathogenesis concurrent gestational diabetes mellitus (GDM) and preeclampsia (PE) (DPE). mRNA expression forkhead box 3 (FoxP3), IL-35 [including Epstein-Barr virus-induced gene (EBI3) P35 subunits] IL-17 peripheral mononuclear cells patients with DPE (n=30), GDM (n=33), PE (n=33) normal pregnancy were...
Cytotoxic T lymphocytes (CTLs) mediate host defense against viral and intracellular bacterial infections tumors. However, the magnitude of CTL response their function needed to confer heterosubtypic immunity influenza virus infection are unknown. We addressed role CD8