Summary Background Adipokines in breast milk have been associated with infant growth trajectories. Objective We aimed to explore the relationship of leptin and adiponectin weight gain body composition up age 2 years. Methods Breast samples were collected from exclusively or partially breastfeeding mothers at 6 weeks ( n = 152) 4 months 120) post‐partum. Leptin determined skim related fat mass assessed by skin‐fold thickness measurements. A total 118 infants examined Results The levels both...

Thus far, clock genes in the heart have been described only rodents, and alterations of these associated with various myocardial malfunctions. In this study, we analyzed expression human hearts. Left papillary muscles 16 patients coronary disease, 39 subjects cardiomyopathy, 9 healthy donors (52 males 12 females, mean age 55.7+/-11.2; 16-70 yrs) were obtained during orthotopic transplantation. We assessed mRNA levels PER1, PER2, BMAL1, CRY1 by real time PCR their rhythmic sliding means...

The presence of a nitrogen atom, charged at physiological pH, has frequently been considered to be hallmark P-glycoprotein (PGP) inhibitors, although certain steroids, such as progesterone, lack atom and still are active modulators PGP. present study was aimed investigating the role plays in activity PGP inhibitors. Propafenone-related amines, anilines, amides that cover broad range pK(a) values, well an ester, were synthesized tested for multidrug resistance-reverting activity. sum hydrogen...

Amphetamine abuse is a major public health concern for which there currently no effective treatment. To develop treatments, the mechanisms by amphetamine produces its abuse-related effects need to be fully understood. It well known that exerts actions targeting high-affinity transporters monoamines, in particular cocaine-sensitive dopamine transporter. Organic cation transporter 3 (OCT3) has recently been found play an important role regulating monoamine signaling. However, whether OCT3...

The sodium channel blocker propafenone and a series of analogs have been identified as effective modulators P-glyco-protein-mediated multidrug resistance in human tumor cells. A closely related structural homologues showed highly significant correlation between lipophilicity pharmacological effect. Reduction the carbonyl group well conversion to methylether led remarkable decrease activity, whereby lost its predictive character main determinant for modulator potency. Similarly, relative...

Cimicifuga racemosa (black cohosh) is commonly used in traditional medicines as treatment for menopausal symptoms and an antiinflammatory remedy. To clarify the mechanism of action active principle action, effects aqueous C. root extracts (CRE) its major constituents on release proinflammatory cytokines IL-6, TNF-alpha, IFN-gamma, chemokine IL-8 were investigated lipopolysaccharide (LPS)-stimulated whole blood healthy volunteers. CRE (3 microg/microL 6 microg/microL) reduced LPS-induced IL-6...

Concurrent cocaine and alcohol use is among the most frequent drug combination, dangerous in terms of deleterious outcomes. Cocaine increases extracellular monoamines by blocking dopamine (DA), norepinephrine (NE) serotonin (5-HT) transporters (DAT, NET SERT, respectively). Likewise, ethanol also monoamines, however evidence suggests that does so independently DAT, SERT. Organic cation transporter 3 (OCT3) an emergent key player regulation monoamine signaling. Using a battery vitro, vivo...

A series of benzofurylethanolamine analogs propafenone (1a) have been prepared and evaluated for multidrug resistance-reversing activity in two vitro assay systems. As propafenones, an excellent correlation biological data with calculated lipophilicity values was found benzofurans, whereby the latter generally had lower activity/lipophilicity ratios. Almost identical slopes regression lines were obtained both propafenones benzofurans. Multiple linear analysis complete set yielded equation...

Patients may develop dual-loop re-entrant atrial arrhythmias late after open-heart surgery, and mapping catheter ablation remain challenging despite computer-assisted techniques.The purpose of the study was to demonstrate prevalence characteristics arrhythmias, define optimal strategy.40 consecutive patients (mean (SD) age 52 (12) years) with intra-atrial tachycardia (IART) surgery (with an incision right free wall) were studied. Dual-loop IART defined as presence two simultaneous circuits....

Background and Purpose Amphetamines bind to the plasmalemmal transporters for monoamines dopamine ( DAT ), noradrenaline NET ) 5‐HT SERT ); influx of amphetamine leads efflux substrates. Various models have been proposed account this amphetamine‐induced reverse transport in mechanistic terms. A most notable example is molecular stent hypothesis, which posits a special conformation that not likely alternative access transport. The current study was designed evaluate explanatory power these...

While classical cathinones, such as methcathinone, have been shown to be monoamine releasing agents at human transporters, the subgroup of α-pyrrolidinophenones has thus far solely characterized transporter reuptake inhibitors. Herein, we report data from previously undescribed α-pyrrolidinopropiophenone (α-PPP) derivatives and compare them with pharmacologically well-researched α-PVP (α-pyrrolidinovalerophenone). Radiotracer-based in vitro uptake inhibition assays HEK293 cells show that...

High density lipoproteins (HDL) represent a protective factor of central importance that counteracts the development cardiovascular disease, in part by normalizing platelet (hyper)reactivity. As HDL an efficient scavenger naturally occurring oxidant hypochlorite, this work was intended to investigate influence hypochlorite-oxidized on function. Addition human platelets results immediate and transient raise intracellular calcium, surface expression P-selectin aggregation. The observed effects...

Immunomodulatory effects of oenothein B (1), a macrocyclic ellagitannin from various Onagraceae species, have been described previously. However, the mechanisms underlying anti-inflammatory activity 1 not fully clarified. The were investigated on inducible nitric oxide synthase, TLR-dependent and TLR-independent signal transduction cascades, cytokine expression using murine macrophages (RAW 264.7). Compound (10–60 μg/mL) reduced NO production, iNOS mRNA, protein levels in dose-dependent...

The multispecific efflux transporter, P-glycoprotein, plays an important role in drug disposition. Substrate translocation occurs along the interface of its transmembrane domains. rotational C2 symmetry ATP-binding cassette transporters implies existence two symmetry-related sets substrate-interacting amino acids. These are identical homodimeric transporters, and remain evolutionary related full such as which substrates bind preferentially, but nonexclusively, to one binding sites. We...

Cytosolic free calcium ions represent important second-messengers in platelets. Therefore, quantitative measurement of intraplatelet provides a popular and very sensitive tool to evaluate platelet activation reactivity. Current protocols for determination intracellular concentrations platelets have number limitations. Cuvette-based methods do not allow flux complex systems, such as whole blood, therefore require isolation steps that potentially interfere with activation. Flow cytometry has...

Journal Article No influence of socioeconomic factors on severe malarial anaemia, hyperparasitaemia or reinfection Get access D. Luckner, Luckner 1Department Parasitology, Institute Tropical Medicine, University Tübingen, Germany2Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon3Department Infectious Diseases, Internal Medicine I, Vienna, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar B. Lell, Lell Gabon Greve, Greve L.G. Lehman, Lehman R.J....

Abstract: Expression of the Wilms' tumour gene (wt1) has been demonstrated in a large proportion human acute leukaemias and is thought to play role leukaemogenesis. Recent observations adult patients with leukaemia suggest that wt1 expression poor prognostic factor. In childhood leukaemia, clinical not established. We have therefore investigated bone marrow samples from 50 children lymphocytic at time diagnosis for presence transcripts determine whether associated specific characteristics...

In 2010 a case of dual infection with Legionella pneumophila serogroup (sg) 1 and sg 3 was identified by culture blood sample collected from female Austrian patient septic pneumonia. Subsequently all 35 European National Reference Laboratories were interviewed regarding the frequency infections in legionellosis. The Denmark, UK Germany reported detection another 14 cases different strains between 2002 2012. Among 15 cases, there four species, six L. serogroups, five MAb-types. median age 56...

Synthetic cathinone derivatives are a new class of psychoactive substances (NPS), also known as "bath salts", designed to exert psychostimulant effects resembling those well-known psychostimulants, such cocaine and 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"). As major constituents bath salts, the 3,4-methylenedioxypyrovalerone (MDPV) 4-methylmethcathinone (mephedrone), have received considerable media attention. MDPV mephedrone interfere with function high affinity transporters for...