A5010215553- Cancer Genomics and Diagnostics
- Occupational and environmental lung diseases
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Neonatal Health and Biochemistry
- Cancer Immunotherapy and Biomarkers
- Global Cancer Incidence and Screening
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Endometriosis Research and Treatment
- Fibroblast Growth Factor Research
- Uterine Myomas and Treatments
- Glioma Diagnosis and Treatment
- Endometrial and Cervical Cancer Treatments
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Pancreatic and Hepatic Oncology Research
- Neonatal and fetal brain pathology
- Breast Cancer Treatment Studies
- Mass Spectrometry Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Radiomics and Machine Learning in Medical Imaging
University of Trieste
1998-2024
Ospedale di Cattinara
2017-2022
Azienda Sanitaria Universitaria Integrata di Trieste
2020
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Ospedale di Monfalcone
2011
Pathological Society
1998
University of Rijeka
1996
Tumor organoids are tridimensional cell culture systems that generated in vitro from surgically resected patients’ tumors. They can be propagated maintaining several features of the tumor origin, including cellular and genetic heterogeneity, thus representing a promising tool for precision cancer medicine. Here, we established patient-derived (PDOs) different breast subtypes (luminal A, luminal B, human epidermal growth factor receptor 2 (HER2)-enriched, triple negative). The model showed...
With the advent of immunotherapies, field cancer therapy has been revived with new hope, especially for cancers dismal prognoses, such as glioblastoma multiforme (GBM). Currently, immunotherapies should potentiate host’s own antitumor immune response against cells, but it documented that they are effective only in small subsets patients. Therefore, accurate predictors urgently needed to identify who will benefit from immune-modulatory therapies. Brain tumors challenging terms treatments. The...
Occupational or environmental exposure to asbestos fibres is associated with pleural and parenchymal lung diseases. A histopathologic hallmark of the presence in parenchyma so-called bodies. They are final product biomineralization processes resulting deposition endogenous iron organic matter (mainly proteins) around inhaled fibres. For shedding light on formation mechanisms bodies it fundamental importance characterize at same length scales not only their structural morphology chemical...
We investigated the correlation between pancreatic ductal adenocarcinoma patient prognosis and presence of tumour infiltrating lymphocytes expression 521 immune system genes.Intratumoural CD3+, CD8+, CD20+ were examined by immunohistochemistry in 12 PDAC patients with different outcomes who underwent pancreaticoduodenectomy. The results correlated gene profile using digital multiplexed NanoString nCounter analysis (NanoString Technologies, Seattle, WA, USA).Twenty genes significantly...
Abstract Pathways that direct the selection of telomerase-dependent or recombination-based, alternative lengthening telomere (ALT) maintenance pathway in cancer cells are poorly understood. Using human lung and tumor organoids we show formation 2,2,7-trimethylguanosine (TMG) cap structure at telomerase RNA 5′ end by Trimethylguanosine Synthase 1 (TGS1) is central for recruiting to telomeres engaging Cajal bodies maintenance. TGS1 depletion inhibition natural nucleoside sinefungin impairs...
The neurologic manifestations of neonatal hyperbilirubinemia in the central nervous system (CNS) exhibit high variations severity and appearance motor, auditory cognitive symptoms, which is suggestive a still unexplained selective topography bilirubin-induced damage. By applying organotypic brain culture (OBC: preserving vitro cellular complexity, connection architecture vivo brain) technique to study hyperbilirubinemia, we mapped regional target damage, demonstrated multifactorial toxic...
Eukaryotic elongation factor 1 alpha 2 (eEF1A2) is a translation selectively expressed by heart, skeletal muscle, nervous system and some specialized cells. Its ectopic expression relates with tumorigenesis in several types of human cancer. No data are available about the role eEF1A2 Triple Negative Breast Cancers (TNBC). This study investigated relation between protein levels prognosis TNBC. A total 84 TNBC diagnosed period 2002-2011 were included study. level was measured formalin-fixed...
Abstract Bilirubin neurotoxicity has been studied for decades and shown to affect various mechanisms via significant modulation of gene expression. This suggests that vital regulatory expression, such as epigenetic mechanisms, could play a role in bilirubin neurotoxicity. Histone acetylation recently received attention the CNS due its numerous biological processes, synaptic plasticity, learning, memory, development differentiation. Aberrant regulation expression psychiatric neurodegenerative...
Endometriosis is a local pelvic inflammatory process, frequently associated with infertility, altered function of immune-related cells in the peritoneal environment. Mast are known to be key players immune system and have been recently involved endometriosis their mediators directly suppressing sperm motility. In this study, we evaluated mast cell population fluid infertile patients impact on human Peritoneal fluids, collected by laparoscopy from 11 9 fertile controls were for presence...
Recent findings indicated aberrant epigenetic control of the central nervous system (CNS) development in hyperbilirubinemic Gunn rats as an additional cause cerebellar hypoplasia, landmark bilirubin neurotoxicity rodents. Because symptoms severely human neonates suggest other regions privileged targets neurotoxicity, we expanded study potential impact on postnatal brain to correlating with symptoms. Histology, transcriptomic, gene correlation, and behavioral studies were performed. The...
The time of death can be established by determining the length postmortem interval. Many methods have been proposed to achieve this goal. Flow cytometric evaluation DNA degradation seems reliable for first 72 hours after death. Our study evaluated correspondence corruption process between in vitro and corpse tissues. We chose spleen tissue perform our investigation because it is rich nucleated cells. Results showed a precise two kinds samples period 24 36 hours. from characterized much...
Breast cancer (BC) is the most common malignancy and second only to lung in terms of mortality women. Despite incredible progress made this field, metastatic breast leaves a poor prognosis. In an era personalized medicine, there urgent need for better knowledge biology leading disease, which can lead design always more accurate drugs against patients’ specific molecular aberrations. Among one actionable targets Fibroblast Growth Factor Receptor (FGFR) pathway, triggered by ligands....
Complement component C1q can act as a pro-tumorigenic factor in the tumor microenvironment (TME). The TME malignant pleural mesothelioma (MPM) is rich and hyaluronic acid (HA), whose interaction enhances adhesion, migration proliferation of cells. HA-bound also capable modulating HA synthesis. Thus, we investigated whether HA-C1q would affect degradation, analyzing main degradation enzymes, hyaluronidase (HYAL)1 HYAL2, receptor candidate. We first proceeded with characterization HYALs MPM...
Freezing—cryosurgery, and electrolysis—electrochemical therapy (EChT), are two important minimally invasive surgery tissue ablation technologies. Despite major advantages they also have some disadvantages. Cryosurgery cannot induce cell death at high subzero freezing temperatures requires multiple freeze thaw cycles, while EChT concentrations of electrolytic products—which makes it a lengthy procedure. Based on the observation that increases concentration solutes (including products...
Bilirubin toxicity to the central nervous system (CNS) is responsible for severe and permanent neurologic damage, resulting in hearing loss, cognitive, movement impairment. Timely effective management of neonatal hyperbilirubinemia by phototherapy or exchange transfusion crucial avoiding neurological consequences, but these therapies are not always possible, particularly low-income countries. To explore alternative options, we investigated a pharmaceutical approach focused on protecting CNS...
Background: In Western countries, breast cancer (BC) is the most common in women. Early detection has a positive impact on survival, quality of life, and public health costs. Mammography screening programs have increased early rates, but new approaches to more personalized surveillance could further improve diagnosis. Circulating cell-free DNA (cfDNA) blood provide potential tool for diagnosis by analyzing cfDNA quantity, circulating tumor mutations, or integrity (cfDI). Methods: Plasma was...
Ferruginous bodies (FB) are polymorphic structures whose formation is macrophage dependent, and composed of a core, which may consist an asbestos fiber coated with proteins, among ferritin the main component. Within ferritin, ferric ferrous ions coordinated as ferrihydrite, iron (Fe) storage compound. However, when accumulates in some tissues following Fe overload it also contains magnetite along endows magnetic properties. Recently studies showed that exerts peroxidase-like activity, since...
DNA repair has an important role in malignant pleural mesothelioma (MPM) tumorigenesis and progression. Prognostic/predictive biomarkers for better management of MPM patients are needed. In the present manuscript, we analyzed expression more than 700 genes a cohort to possibly find correlated with survival.A total 54 patients, all epithelioid histology, whose survival follow-up formalin-fixed paraffin-embedded tumors were available, included study. Gene profiles evaluated using Nanostring...
Her2 protein is the key marker determining choice of Herceptin therapy after a diagnosis breast cancer. Its evaluation made in most laboratories by immunohistochemistry, and interpreted pathologist using an optical microscope, process subject to inter-observer variability, particularly for samples scored as equivocal (2+). Software analysis products have been introduced, seeking reduce this variability. In study, we compared results both traditional specific software package (VISIA Imaging)...
Although the eukaryotic elongation factor eEF1A1 plays a role in various tumours, there is little information on its prognosis/therapeutic value prostate carcinoma. In high-grade and castration-resistant carcinoma (CRPC), identification of novel therapeutic markers/targets remains priority. The expression protein was determined formalin-fixed, paraffin-embedded cancer hyperplasia tissue by IHC. investigated cellular model using DNA aptamer (GT75) we previously developed. We used aggressive...
Introduction and Objectives. Analysis of cancer biomarkers is an important tool in developing targeted-therapy modulating chemoresistance. Here, we analyze the relevance CD90, a marker stem cells (CSC) hepatocellular carcinoma (HCC) its correlation with autophagy. Materials Methods. For vivo study, 86 specimens were collected from 43 patients undergoing liver resections. In each patient, HCC nodule surrounding non-tumor (SNT) collected. vitro JHH6 subpopulations expressing CD90+ CD90-...