A5051437290- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- Gut microbiota and health
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Parasites and Host Interactions
- Lymphatic System and Diseases
- Hepatitis B Virus Studies
- Immune cells in cancer
- NF-κB Signaling Pathways
- Dietary Effects on Health
- Signaling Pathways in Disease
- interferon and immune responses
- Cellular Mechanics and Interactions
- Yersinia bacterium, plague, ectoparasites research
- Inflammatory Bowel Disease
- Parasitic Diseases Research and Treatment
- Immunotherapy and Immune Responses
- Cerebrospinal fluid and hydrocephalus
Regeneron (United States)
2022-2023
National Institute of Allergy and Infectious Diseases
2015-2023
University of Pennsylvania
2009-2022
National Institutes of Health
2015
The relationship of T follicular helper (TFH) cells to other (Th) subsets is controversial. We find that after helminth infection, or immunization with antigens, reactive lymphoid organs 4get IL-4/GFP reporter mice contain populations IL-4/GFP-expressing CD4+ display the TFH markers CXCR5, PD-1, and ICOS. These express canonical BCL6 IL-21, but also GATA3, master regulator Th2 cell differentiation. Consistent a between cells, IL-4 protein production, reported by expression huCD2 in dual...
Long-lived plasma cells (PCs) in the bone marrow (BM) are a critical source of antibodies after infection or vaccination, but questions remain about factors that control PCs. We found systemic alters BM, greatly reducing PCs and regulatory T (Treg) cells, population contributes to immune privilege BM. The use intravital imaging revealed BM Treg display distinct behavior characterized by sustained co-localization with CD11c-YFP+ cells. Gene expression profiling indicated express high levels...
The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling and play crucial roles in various cellular processes. We determined cryo–electron microscopy structures of five complexes this family, containing full ectodomains bound to their respective ligands ciliary neurotrophic factor, cardiotrophin-like cytokine factor 1 (CLCF1), leukemia inhibitory IL-27, IL-6. Our collectively reveal similarities differences the...
The transcription factor T-bet has been most prominently linked to NK and T cell production of IFN-γ, a cytokine required for the control diverse array intracellular pathogens. Indeed, in mice challenged with parasite Toxoplasma gondii, responses are characterized by marked increases expression. Unexpectedly, T-bet(-/-) infected T. gondii develop strong IFN-γ response that controls replication at challenge site, but display high burdens secondary sites colonized succumb infection. loss had...
ABSTRACT B cell responses are required for resistance to Toxoplasma gondii ; however, the events that lead production of class-switched antibodies during T. infection have not been defined. Indeed, mice challenged with parasite exhibited an expansion T follicular helper cells and germinal center in spleen. Unexpectedly, this was associated formation instead accompanied by profound changes splenic organization. This phenomenon transient correlated a decrease expression effector proteins...
The microbiota performs multiple functions vital to host fitness, including defense against pathogens and adaptation dietary changes. Yet, how environmental challenges shape resilience nutrient fluctuation remains largely unexplored. Here, we show that transient gut infection can optimize metabolism toward the usage of carbohydrates. Following acute clearance pathogen, mice gained more weight as a result white adipose tissue expansion. Concomitantly, previously infected exhibited enhanced...
Abstract The gp130 family cytokine signaling complexes have limited structural information despite their crucial roles in various cellular processes. We determined cryo-EM structures of several this family, containing full ectodomains both receptors bound to respective ligands CNTF, CLCF1, LIF, IL-27, and IL-6. Our reveal that serves as a central receptor by engaging Site 2 IL-6, 3 IL-27 acute bends at all bring the membrane-proximal domains ~30 Å range but with distinct distances...
Abstract The lymphatic system facilitates critical processes for immunity, including immune cell trafficking, lipid transport, and the maintenance of tissue homeostasis. In mesentery, these vessels (LVs) allow drainage trafficking from gastrointestinal (GI) tract through mesenteric adipose (mAT) to lymph nodes (mLNs). During GI infection, this vasculature is altered by inflammation, which can induce leakage. Repair LVs after infection resolved necessary restore function. Although preliminary...
Abstract The white adipose tissue represents 15% of healthy mammalian hosts. In addition to serving as a scaffold for the lymphatic and blood vasculature, this compartment plays fundamental role in control host metabolism. To which extent also contributes immune surveillance long-term protective defense remains largely unknown. Our studies show that under steady state conditions, is home abundant diverse effector memory lymphocyte populations. Following infection, accumulates large numbers...