A5087470177- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Advanced Chemical Sensor Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Algal biology and biofuel production
- Polymer composites and self-healing
- Carbon Nanotubes in Composites
- Innovative Microfluidic and Catalytic Techniques Innovation
- Polymer Foaming and Composites
- Carbon dioxide utilization in catalysis
- Single-cell and spatial transcriptomics
- Mesoporous Materials and Catalysis
- Lipid Membrane Structure and Behavior
- Diet and metabolism studies
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Adenosine and Purinergic Signaling
- Biomedical Research and Pathophysiology
- Photosynthetic Processes and Mechanisms
- Liver Disease Diagnosis and Treatment
- Isotope Analysis in Ecology
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Mechanical and Optical Resonators
Kyushu University
2018-2025
Hiroshima University
2020
Kyoto University
2009-2011
Osaka Prefecture University
2009
Tokyo Metropolitan University
1973
Omega-3 polyunsaturated fatty acids have anti-inflammatory effects in vitro, and high dietary levels are associated with a lower incidence of inflammatory diseases. However, only limited been demonstrated asthma. The supplementation fish oil for 10 months 29 children bronchial asthma was investigated randomized controlled fashion. In order to minimize the environmental inhaled allergens diet, this study performed long-term treatment hospital. Subjects received capsules containing 84 mg...
Lipoproteins, which are synthesized in hepatic cells and secreted into the blood, play an important role lipid transport. The quality of lipoproteins, including small dense low-density lipoproteins cholesterol efflux capacity high-density influences cardiovascular outcomes, although relationship between changes lipoprotein remains unclear. This study characterized molecules apolipoproteins particles during development coronary atherosclerosis myocardial infarction-prone rabbits. Using...
Background: One of the current problems in field metabolomics is difficulty integrating data collected using different equipment at facilities, because many metabolomic methods have been developed independently and are unique to each laboratory. Methods: In this study, we examined whether analytical among 12 laboratories provided comparable relative quantification for certain metabolites. Identical samples extracted from two cell lines (HT-29 AsPc-1) were distributed facility, hydrophilic...
Several studies in hepatocyte cell lines reported that medium-chain fatty acids (MCFAs) with 6–12 carbons showed different metabolic properties from long-chain (LCFAs). However, these unclear effects of acid molecules on metabolism. This study is aimed to capture the kinetics MCFA assimilation AML12 cells treated octanoic (FA 8:0), decanoic 10:0), or lauric (FA12:0) [LCFA; oleic 18:1)] via profiling and dynamic metabolome analysis 13C-labeling. The concentrations total ketone bodies media FA...
The rapid development of next-generation sequencing techniques has enabled single-cell genomic and transcriptomic analyses, which have revealed the importance heterogeneity in biological systems. However, analytical methods to accurately identify quantify comprehensive metabolites from single mammalian cells with a typical diameter 10-20 μm are still process development. aim this study was develop metabolomic system based on highly sensitive nano-liquid chromatography tandem mass...
Phospholipids are asymmetrically distributed in the plasma membrane (PM), with phosphatidylcholine and sphingomyelin abundant outer leaflet. However, mechanisms by which their distribution is regulated remain unclear. Here, we show that transmembrane protein 63B (TMEM63B) functions as a structure-responsive lipid scramblase localized at PM lysosomes, activating bidirectional translocation upon changes curvature thickness. TMEM63B contains two intracellular loops palmitoylated cysteine...
Omics analysis at single-cell resolution has helped to demonstrate the shaping of cellular heterogeneity on basis expression various molecules. However, in-depth proteomic low-quantity samples remained challenging because difficulties associated with measurement large numbers proteins by shotgun proteomics using nanoflow liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). To meet such a demand, we developed method called in-line sample preparation for efficient (ISPEC) in which...
The acetylation of histone tails is a key factor in the maintenance chromatin dynamics and cellular homeostasis. hallmark active hyper-acetylation histones, which appears to result more open structure. Although short nucleosomal arrays have been studied, structural relatively long acetylated remain unclear. We analyzed detail structure hyper-acetylated fibers using atomic force microscopy (AFM). Hyper-acetylated isolated from nuclei that had treated with Trichostatin A (TSA), an inhibitor...
The response of a carbon nanotube field effect transistor (CNT-FET) to vibrating gate has been investigated by scanning microscopy (SGM) with frequency modulation (FM) mode in air at room temperature. Topography and current images were obtained simultaneously. CNT-FET successfully detected cantilever vibration an amplitude less than 50 nm afrequency 80 kHz.
Pre-column fluorescent derivatization has been used for the fast quantification of amino acids using high-performance liquid chromatography (HPLC) systems. However, it generally requires an offline in-vial process with multiple reagents. The same number reaction vials as sample use a chamber in autosampler. Therefore, samples analyzed per batch pre-column method is halved. To benefit from method, we transformed to online in-needle (in-needle Derivatization Amino Quantification; iPDAQ)....
In mass spectrometry-based metabolomics, the differences in analytical results from different laboratories/machines are an issue to be considered because various types of machines used each laboratory. Moreover, methods unique It is important understand reality inter-laboratory metabolomics. Therefore, we have evaluated whether methods, with exception sample pretreatment and including metabolite extraction, involved or not. this study, nine facilities for comparisons metabolomic analysis....
ABSTRACT Lipogenesis-driven metabolic flux is crucial for differentiation of pathogenic Th17 cells. Although our previous CRISPR-based screening identified PLA2G12A as a key player in this process, it has remained obscure how secreted phospholipase A 2 isoform controls differentiation. Here we show that global, T cell-specific, or fibroblast-specific deletion prevents and associated diseases including psoriasis arthritis. acts on Th17-derived extracellular vesicles (EVs) to produce...
Calibration-Curve-Locking Databases (CCLDs) have been constructed for automatic compound search and semi-quantitative screening by gas chromatography/mass spectrometry (GC/MS) in several fields. CCLD felicitates the semi-quantification of target compounds without calibration curve preparation because it contains retention time (RT), curves, electron ionization (EI) mass spectra, which are obtained under stable apparatus conditions. Despite its usefulness, there is no metabolomics. Herein, we...