A5058842318- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Tryptophan and brain disorders
- Genomics, phytochemicals, and oxidative stress
- Biochemical Acid Research Studies
- Microtubule and mitosis dynamics
- Toxic Organic Pollutants Impact
- Eicosanoids and Hypertension Pharmacology
- Ferroptosis and cancer prognosis
- Cancer-related cognitive impairment studies
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Circadian rhythm and melatonin
- Diet and metabolism studies
- Selenium in Biological Systems
- ATP Synthase and ATPases Research
- Trace Elements in Health
- Genetic Neurodegenerative Diseases
- Heavy Metal Exposure and Toxicity
- Chemotherapy-induced organ toxicity mitigation
- Light effects on plants
- Aluminum toxicity and tolerance in plants and animals
- Nanoparticles: synthesis and applications
- Cardiovascular Health and Risk Factors
Heinrich Heine University Düsseldorf
2020-2024
Leibniz Institute of Environmental Medicine
2015-2024
University of Münster
2017
The ubiquitously expressed aryl hydrocarbon receptor (AhR) induces drug metabolizing enzymes as well regulators of cell growth, differentiation and apoptosis. Certain AhR ligands promote atherosclerosis, an age-associated vascular disease. Therefore, we investigated the role in functionality aging. We report a lower pulse wave velocity young old AhR-deficient mice, indicative enhanced vessel elasticity. Moreover, endothelial nitric oxide synthase (eNOS) showed increased activity aortas these...
Prunus spinosa L. fruit (PSF) ethanol extract, showing a peculiar content of biologically active molecules (polyphenols), was investigated for its wound healing capacity, typical feature that declines during aging and is negatively affected by the persistence inflammation oxidative stress. To this aim, first, PSF anti-inflammatory properties were tested on young senescent LPS-treated human umbilical vein endothelial cells (HUVECs). As result, treatment increased miR-146a decreased IRAK-1...
Abstract Eukaryotic elongation factor 2 (eEF2) kinase (eEF2K) is a stress-responsive hub that inhibits the translation eEF2, and consequently mRNA elongation, in response to hypoxia nutrient deprivation. EEF2K also involved DNA damage but its role crosslinks, as induced by cisplatin, not known. Here we found eEF2K critical mediate cellular cisplatin. We uncovered deficient cells are more resistant cisplatin treatment. Mechanistically, deficiency blunts activation of associated ATM ATR...
Abstract Complex-I-deficiency represents the most frequent pathogenetic cause of human mitochondriopathies. Therapeutic options for these neurodevelopmental life-threating disorders do not exist, partly due to scarcity appropriate model systems study them. Caenorhabditis elegans is a genetically tractable organism widely used investigate neuronal pathologies. Here, we generate C. models mitochondriopathies and show that depletion complex I subunits recapitulates biochemical, cellular aspects...
Excessive iron accumulation or deficiency leads to a variety of pathologies in humans and developmental arrest the nematode Caenorhabditis elegans. Instead, sub-lethal depletion extends C. elegans lifespan. Hypoxia preconditioning protects against severe hypoxia-induced neuromuscular damage across species but it has low feasible application. In this study, we assessed potential beneficial effects genetic chemical interventions acting via mild instead oxygen depletion. We show that limiting...
The mechanisms of cadmium (Cd) resistance are complex and not sufficiently understood. present study, therefore, aimed at assessing the roles important components stress-signaling pathways ABC transporters under severe Cd stress in Caenorhabditis elegans. Survival assays on mutant control animals revealed a significant promotion by PMK-1 p38 MAP kinase, transcription factor DAF-16/FoxO, transporter MRP-1. Transcriptome profiling RNA-Seq wild type pmk-1 conditions revealed, inter alia,...
Aging is one of the main risk factors for neurodegenerative disorders, which represent a global burden on healthcare systems. Therefore, identifying new strategies to slow progression brain aging compelling challenge. In this article, we first assessed potential anti-aging effects Citrus flavanone naringenin (NAR), an activator enzyme sirtuin-1 (SIRT1), in 3R-compliant and short-lived model (i.e., nematode C. elegans). Then, investigated preventive 6-month treatment with NAR (100 mg/kg,...
Genetic, dietary, and environmental factors concurrently shape the aging process. The aryl hydrocarbon receptor (AhR) was discovered as a dioxin-binding transcription factor involved in metabolism of different toxicants vertebrates. Since then, variety pathophysiological processes regulated by AhR has grown, ranging from immune response, metabolic pathways, aging. Many modulators activity may impact on age-associated pathologies, but, whether their effects are AhR-dependent never been...
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor whose activity can be modulated by polyphenols, such as curcumin. AhR and curcumin have evolutionarily conserved effects on aging. Here, we investigated whether how the mediates anti-aging of across species. Using combination in vivo, vitro, silico analyses, demonstrated that has AhR-dependent or -independent context-specific manner. We found Caenorhabditis elegans, curcumin-induced lifespan extension, most likely...
The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR invertebrates. In C. elegans, ortholog, AHR-1, displays physiological functions. Therefore, we compared transcriptomic and metabolic profiles worms expressing AHR-1 or not investigated putative panel chemical modulators. metabolomic profiling indicated role amino acids, carbohydrates, fatty acids metabolism....
Abstract Complex I deficiency represents the most frequent pathogenetic cause of human mitochondriopathies. Therapeutic options for these neurodevelopmental life-threating disorders do not exist, partly due to scarcity appropriate model systems study them. Caenorhabditis elegans is a genetically tractable organism widely used investigate neuronal pathologies. Here, we generated new C. models mitochondriopathies and showed that depletion subunits recapitulates biochemical, cellular aspects...
Although manganese (Mn) is an essential trace element, overexposure associated with Mn-induced toxicity and neurological dysfunction. Even though oxidative stress discussed extensively, neither the underlying mechanisms of potential consequences on DNA damage repair, nor possibly resulting are characterized yet. In this study, we use model organism Caenorhabditis elegans to investigate mode action Mn toxicity, focusing genomic integrity by means response. Experiments were conducted analyze...
Abstract The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor whose activity can be modulated by polyphenols such as curcumin. AhR and curcumin have evolutionarily conserved effects on aging. Here, we investigated whether how the mediates anti-aging of across species. Using combination in vivo, vitro , silico analyses, demonstrated that has AhR-dependent or -independent context-specific manner. We found Caenorhabditis elegans curcumin-induced lifespan extension,...
Abstract Genetic and environmental factors concurrently shape the aging process. The nematode Caenorhabditis elegans has been instrumental in past 30 years to identify most genes interventions nowadays known modulate (Lopez-Otin et al. 2013, Tigges 2014, Dato 2017). aryl-hydrocarbon-receptor (AhR) was discovered as a dioxin-binding transcription factor involved metabolism of different toxicants xenobiotics (Poland 1976). Since then, variety pathophysiological processes regulated by AhR...
Development of age-associated vascular diseases like atherosclerosis depends not only on genetic predisposition but also environmental influences. Ligands the aryl hydrocarbon receptor (AhR), a ubiquitously expressed transcription factor upregulating detoxifying enzymes, dioxin and benzo[a]pyrene (BaP) have been shown to promote atherosclerosis. Furthermore, recovery blood flow after hindlimb ischemia is significantly enhanced in AhR-deficient mice demonstrating increased angiogenesis...
Severe oxygen and iron deficiency have evolutionarily conserved detrimental effects, leading to pathologies in mammals developmental arrest as well neuromuscular degeneration the nematode C. elegans. Yet, similar beneficial effects of mild hypoxia, non-toxic levels depletion, achieved with chelator bipyridine or through frataxin silencing, extend elegans lifespan hypoxia like-induction mitophagy. The positive health outcomes applying preconditioning are obvious but low feasible application....