Background The great interest in cell-free mRNA, microRNA (miRNA) as molecular biomarkers for clinical applications, and 'signaling' molecules intercellular communication highlights the need to reveal their physical nature. Here this issue was explored human seminal mRNA (cfs-mRNA) miRNA (cfs-miRNA). Methodology/Principal Findings Selected male reproductive organ-specific mRNAs, miRNAs, piRNAs were quantified by quantitative real-time PCR all experiments. While stability of cfs-miRNA...

Abstract The maintenance of genome integrity in the germline is crucial for mammalian development. Long interspersed element type 1 (LINE-1, L1) a mobile genetic that makes up about 17% human and poses threat to integrity. N6-methyl-adenosine (m 6 A) plays an essential role regulating various biological processes. However, function m A modification L1 retrotransposons development remains largely unknown. Here we knocked out methyltransferase METTL3 or reader YTHDF2 embryonic stem cells...

Background The age-related decline in testosterone levels is thought to be of great importance for male aging and cardiovascular diseases. However, data are controversial on whether abnormal sex hormones linked the presence diseases it also uncertain how blood pressure modifies association between major Methods Results This a multicenter, population-based, cross-sectional study 6296 men conducted 2013 2016. Basic information clinical symptoms were obtained by questionnaires. Blood plasma...

We recently detected cell-free seminal RNA (cfsRNA) and set out to study its concentration, integrity, stability in healthy individuals, mechanisms for protection from ribonucleases.We quantified cfsRNA by reverse-transcription quantitative real-time PCR (RT-qPCR) targeting of the 5' region ACTB (actin, beta) transcript. integrity was analyzed microcapillary electrophoresis amplification full-length DDX4 [DEAD (Asp-Glu-Ala-Asp) box polypeptide 4] transcripts, including measurement relative...

DNA methylation analysis is useful for investigation of male fertility in mammals, whereas the reliance on tissues limits research human. We have previously found presence high concentration cell-free seminal (cfsDNA) human semen. proposed that some testis and epididymis-specific methylated promoters could be detected cfsDNA, thus hold promise as noninvasive epigenetic biomarkers infertility, which most cases are caused by defects testicular sperm production or epididymal maturation.The...

The present study aims to analyze sperm concentration trends among young and healthy Chinese adults in Wuhan, Central China, from 2010 2015. Semen analysis data 9357 participants were collected analyzed using a general linear model the Cochran-Armitage trend test. A significant decline was observed (β [standard deviation]: -1.53 [0.16]; P < 0.001). In addition, density by stratifying student versus nonstudent donors analyzing year of birth or cohort participants. Furthermore, percentage with...

Comprehensive analyses showed that SARS-CoV-2 infection caused COVID-19 and induced strong immune responses sometimes severe illnesses. However, cellular features of recovered patients long-term health consequences remain largely unexplored. In this study, we collected peripheral blood samples from nine (median age 36 years old) Hubei province, China, 3 months after discharge as well 5 age- gender-matched healthy controls; carried out RNA-seq whole-genome bisulfite sequencing to identify...

Urokinase-type plasminogen activator (uPA) is closely related to male reproduction. With the aim of investigating possibility for uPA as a potential contraceptive target, in present work, Kunming mice were immunized by human subcutaneous injection at three separate doses 3 times. Then potency anti-human antibody serum was analyzed, and mouse fertility evaluated. Serum titers groups all reached 1:10,240 or higher levels enzyme linked immunosorbent assay, mating experiments revealed that...

Oligoasthenospermia (OAZ) is the most common element contributing to male infertility. However, etiology of OAZ remains unknown in majority cases. Growing evidence indicates that exosomal circular (circ)RNAs may exhibit potential as biological markers for detection various disorders. The available information on exosomes derived from seminal plasma limited. present study investigated composition and role circRNAs isolated patients with OAZ. Exosomes were 12 matched healthy controls....

Can we identify diurnal oscillations in human semen parameters as well peak times of quality?

Abstract Background The most serious condition of male infertility is complete Sertoli cell-only syndrome (SCOS), which refers to the lack all spermatogenic cells in testes. genetic cause SCOS remains be explored. We aimed investigate and assess effects identified causative variant on human germ cells. Methods Whole-exome sequencing was performed identify potentially pathogenic variants a man with SCOS, Sanger verify this his father brother. mechanisms were investigated by vitro...

Mitofusin 2 is a kind of mitochondria membrane protein that has been implicated in maintenance mitochondrial morphology and function. However, the expression function mitofusin human sperm are not well described at present. The aim this study was to explore location discover its relationship functions like motility cryoprotective potentials. Our result showed specifically localized 5–7 μm midpiece between neck main part tail. level significantly different normozoospermia asthenozoospermia...

Abstract Background In mammals, specification of primordial germ cells (PGCs) is established in the early post­implantation embryo. The bone morphogenetic protein (BMP)-SMAD and WNT3-β-catenin signaling initiate gene regulatory network for PGC specification. activation SOX17-BLIMP1 axis critical human program. Moreover, EpCAM INTEGRINα6 were identified as surface markers PGC-like (PGCLCs) recently. However, mechanism non­rodent mammals remains to be clarified. Methods We differentiated...

Environmental pollution has emerged as a global concern due to its detrimental effects on human health. One of the critical aspects this is impact environmental sperm quality in males. Male factor infertility accounts for approximately 40%- 50% all cases. Nonobstructive azoospermia (NOA) most severe type male infertility. Human umbilical cord mesenchymal stem cell (hUCMSC) exosomes enhance proliferation and migration, playing crucial roles tissue organ injury repair. However, whether hUCMSC...

Human primordial germ cells (hPGCs) initiate from the early post-implantation embryo at week 2-3 and undergo epigenetic reprogramming during development. However, regulatory mechanism of DNA methylation hPGC specification is still largely unknown due to difficulties in analyzing human embryos. Using an vitro model induction, we found a novel function TET proteins NANOG which was different that discovered mice.Using CRISPR-Cas9 system, generated set TET1, TET2 TET3 knockout H1 embryonic stem...

Mahogunin is an important mediator of chromogenesis and neurodegeneration. Mahoganoid a mutation the mahogunin gene, which causes pleiotropic phenotype that includes suppression obesity, spongiform neurodegeneration improvement insulin sensitivity. Our previous research found mahoganoid widely expressed in male rat reproductive system, mahoganoid-deficient mice have reduced embryonic viability. But change knockout (md(nc) ) has not been reported previously. Here, we report mRNA also exists...