A5069298555- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Contraception
- Gynecological conditions and treatments
- Endometriosis Research and Treatment
- Ovarian function and disorders
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Heat shock proteins research
- Pregnancy and preeclampsia studies
- Nanoparticles: synthesis and applications
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Pregnancy and Medication Impact
- MicroRNA in disease regulation
- Heavy Metal Exposure and Toxicity
- Orthopaedic implants and arthroplasty
- Protein Tyrosine Phosphatases
- Sexual function and dysfunction studies
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- GDF15 and Related Biomarkers
Huazhong University of Science and Technology
2014-2024
Hangzhou Cancer Hospital
2023-2024
Southwest University
2022-2023
Shanghai Jiao Tong University
2012-2019
The Medical Device (United Kingdom)
2018
Seattle Reproductive Medicine
2018
Laser Research Institute
2014
XinHua Hospital
2009
Army Medical University
2008
Institute of Zoology
1998
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on male reproductive organs, from perspective sperm parameters, semen plasma biochemical markers, and oxidative stress, evaluate whether different frequencies heat exposure cause degrees damage spermatogenesis. Two groups volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: consecutive days; 2: once every 3 days. Sperm...
Large studies on semen quality of the general healthy population from China are rare.A cross-sectional study was performed to evaluate 1346 men residing in Chongqing area southwest 2007. The parameters were measured and compared with World Health Organization (WHO) criteria. A linear regression model used examine determinants quality.The medians 2.3 ml for volume, 77.8 x 10(6) per concentration, 167.7 total sperm count, 33% rapid progressive motility, 52.6% motility 70.9% motility. According...
Infection and inflammation of the male reproductive tract are thought to be a primary aetiological factor infertility. Furthermore, several studies suggest that T lymphocytes critically involved as regulator in pathogenesis infertility under these conditions induce autoimmune orchitis. In this context autoimmunity recently described helper (Th) 17 subset has been suggested play an essential role so aim study was investigate expression characteristics Th17 cells well presence inducing antigen...
// Ming Xu 1,2 , Changhong Zhu 2 Xian Zhao Cheng Chen Hailong Zhang Haihua Yuan 1 Rong Deng Jinzhuo Dou Yanli Wang Jian Huang Qin Bin Jiang and Jianxiu Yu 2,3 Institute of Oncology & Department Oncology, Shanghai 9th People's Hospital, Jiao Tong University School Medicine, Shanghai, China Biochemistry Molecular Cell Biology Key Laboratory Tumor Microenvironment Inflammation, 3 State Oncogenes Related Genes, Correspondence: Yu, email: Keywords : Epithelial-mesenchymal transition (EMT),...
Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, whereas pathogenesis still not fully understood. Systemic and ovarian oxidative stress (OS) imbalance a pivotal feature PCOS. Humanin, mitochondria-derived peptide, has been reported to function as an antioxidant in cardiomyocytes, pancreatic beta cells other cells, but how this regulated remains unclear. In study, we investigated whether humanin expression differs granulosa (GCs)...
Grb2 (Growth factor receptor-bound protein 2) is a key adaptor in maintaining the ERK activity via linking Sos1 (Son of sevenless homolog 1) or other proteins to activated RTKs, such as EGFR. Currently, little knowledge available concerning post-translational modification (PTM) except for its phosphorylation. Since emerging evidences have highlighted importance SUMOylation (Small ubiquitin-related modifier), reversible PTM, modulating functions, we wondered if could be SUMOylated and thereby...
Copper nanoparticle-induced ovarian injury, follicular atresia, apoptosis, and gene expression alterations in female rats Jing Yang,1,* Shifu Hu,1,* Meng Rao,1 Lixia Hu,2 Hui Lei,1 Yanqing Wu,1 Yingying Wang,1 Dandan Ke,1 Wei Xia,1,3 Chang-hong Zhu1,3 1Family Planning Research Institute, Tongji Medical College, Huazhong University of Science Technology, Wuhan, Hubei, 2Department Histology Embryology, Preclinical Medicine Xinxiang University, Henan Province, Xinxiang, 3Reproductive Center,...
The toxicity of copper nanoparticle (CuNP) exposure in the ovaries has attracted attention recently, but precise molecular mechanism involved requires further investigation. We investigated cytotoxicity CuNPs ovarian granulosa cells and protective effect heme oxygenase 1 (HO-1) against CuNP-induced damage.Human (COV434) were treated with CuNPs, was evaluated using Cell Counting Kit-8 flow cytometry assays. Oxidative stress identified biochemical markers oxidation anti-oxidation. protein...
Abstract STUDY QUESTION Does a humanin analogue (HNG) have therapeutic effect on intrauterine adhesions (IUAs) caused by uterine cavity surgery in rat model? SUMMARY ANSWER HNG supplementation attenuated the development of endometrial fibrosis and IUAs, improved fertility, contributed to regulation inhibiting ferroptosis rats with IUAs. WHAT IS KNOWN ALREADY which are characterized fibrosis, common cause female infertility. Humanin (rattin rats) is mitochondrial-derived peptide that widely...
DGCR8 (DiGeorge syndrome critical region gene 8) is essential for primary microRNA (pri-miRNA) processing in the cell nucleus. It specifically combines with Drosha, a nuclear RNase III enzyme, to form Microprocessor complex (MC) that cleaves pri-miRNA precursor miRNA (pre-miRNA), which further processed mature by Dicer, cytoplasmic enzyme. Increasing evidences suggest pri-/pre-miRNAs have direct functions regulation of expression, however underlying mechanism how it fine-tuned remains...
Abstract Small RNA-induced gene silencing is essential for post-transcriptional regulation of expression; however, it remains unclear how miRNA/siRNA efficiency regulated. Here we show that TARBP2 SUMOylated at K52, which can be enhanced by its phosphorylation. This modification stabilize via repressing K 48 -linked ubiquitination. We find SUMOylation does not influence the overall production mature miRNAs, but regulates efficiency. recruits Ago2 to constitute complex (RISC)-loading (RLC),...
Abstract Background Through this prospective study, we aimed to explore the change of molecular modification after transient scrotal hyperthermia on human sperm. Methods Ten healthy subjects selected with strict screening criteria underwent testicular warming in a 43 °C water bath for 30 min day 10 consecutive days. Semen samples were collected 2 weeks before first heat treatment and 6 treatment. Proteins from labeled isobaric tags relative absolute quantitation analyzed by two-dimensional...
Abstract Background Intrauterine adhesion (IUA) is one of the leading causes infertility and main clinical challenge high recurrence rate. The key to solving this dilemma lies in elucidating mechanisms endometrial fibrosis. aim our team study mechanism underlying intrauterine fibrosis origin fibroblasts repair Methods Our experimental involving an animal model detection fibrosis-related molecules. levels molecular factors related endothelial-to-mesenchymal transition (EndMT) were examined a...
DNA methylation analysis is useful for investigation of male fertility in mammals, whereas the reliance on tissues limits research human. We have previously found presence high concentration cell-free seminal (cfsDNA) human semen. proposed that some testis and epididymis-specific methylated promoters could be detected cfsDNA, thus hold promise as noninvasive epigenetic biomarkers infertility, which most cases are caused by defects testicular sperm production or epididymal maturation.The...
The aim of this study was to evaluate the semen quality university students in Wuhan, largest city world terms number students. All student sperm donors recorded Hubei Province Human Sperm Bank from 1 March 2010 31 December 2013 were screened. At last, a total 3616 samples 1808 eligible and retrospectively analyzed. Each donor's parameters averaged over two compared with World Health Organization criteria, generalized linear regression model used examine several determinants quality. We...
// Rong Deng 1 , Xian Zhao YingYing Qu Cheng Chen Changhong Zhu Hailong Zhang Haihua Yuan Hui Jin Xin Liu Yanli Wang Qin Jian Huang Jianxiu Yu 1, 2, 3 Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory Tumor Microenvironment Inflammation, Jiao Tong University School Medicine, 200025, China 2 State Oncogenes Related Genes, Institute Oncology & Oncology, 9th People's Hospital, Correspondence to: Yu, e-mail: Jianxiu.Yu@gmail.com Keywords: Shp2, SUMOylation, Gab1, ERK...
// Meng Rao 1, * , Zhengyan Zeng 2, Li Tang 1 Guiping Cheng 3 Wei Xia and Changhong Zhu Department of Reproduction Genetics, The First Affiliated Hospital Kunming Medical University, Kunming, China 2 Neurology, Family Planning Research Institute, Tongji College, Huazhong University Science Technology, Wuhan, These authors have contributed equally to this work Correspondence to: Rao, email: rm3816205@163.com Keywords: heat stress; spermatogenesis; microRNA; next-generation sequencing;...