// Ming Xu 1,2 , Changhong Zhu 2 Xian Zhao Cheng Chen Hailong Zhang Haihua Yuan 1 Rong Deng Jinzhuo Dou Yanli Wang Jian Huang Qin Bin Jiang and Jianxiu Yu 2,3 Institute of Oncology & Department Oncology, Shanghai 9th People's Hospital, Jiao Tong University School Medicine, Shanghai, China Biochemistry Molecular Cell Biology Key Laboratory Tumor Microenvironment Inflammation, 3 State Oncogenes Related Genes, Correspondence: Yu, email: Keywords : Epithelial-mesenchymal transition (EMT),...

ABSTRACT Prostate cancer (PCa) is the most prevalent malignant carcinoma among males in western countries. Currently no treatments can cure advanced prostate cancers, so new diagnostic and therapeutic strategies are urgent need. At present limited knowledge available concerning roles of dysregulated microRNAs metastasis. In this study, we found that expression miR‐130b was significantly down‐regulated cell lines clinical tissues. Enforced over‐expression cells suppressed whereas knock‐down...

Grb2 (Growth factor receptor-bound protein 2) is a key adaptor in maintaining the ERK activity via linking Sos1 (Son of sevenless homolog 1) or other proteins to activated RTKs, such as EGFR. Currently, little knowledge available concerning post-translational modification (PTM) except for its phosphorylation. Since emerging evidences have highlighted importance SUMOylation (Small ubiquitin-related modifier), reversible PTM, modulating functions, we wondered if could be SUMOylated and thereby...

Doxorubicin, an anthracycline commonly used for treating cancer patients, is known its cardiotoxic side-effects. Although dose-dependent, but susceptibility remains variable among and childhood-exposure-adult-onset challenging. Besides topoisomerase toxicity, Doxorubicin also toxic to the mitochondria yet underlying late onset mechanism elusive. Here, it observed that mitochondrial copy number in PBMCs of patients treated with chemotherapy negatively correlated change plasma BNP levels after...

Prostate cancer (PCa) is the second leading cause of cancer-related deaths in north American men, and most its related are due to advanced metastatic PCa. However, molecular mechanisms underlying PCa progression still unclear. Here we use a pair prostate cell lines P69/M12, which have same genetic background highly line M12 subline derived from P69, identify pathogenesis We find that key miRNA--miR186 significantly reduced compared P69. Further, validate miR186 also downregulated human...

This study aimed to evaluate the prognostic value of circulating tumor cells (CTCs) in advanced colorectal cancer (CRC) patients during chemotherapy course. From January 2016 September 2017, clinicopathological variables, such as gender, age, location, de-differentiation, depth invasion, lymphatic distant metastasis, TNM stage, CTCs enumeration 2-6 cycles chemotherapy, and serum carcinoembryonic antigen (CEA) level same period, 121 newly acquired histopathologically confirmed CRC were...

Epidermal growth factor receptor gene (EGFR) mutations are among the best predictive markers of efficacy EGFR tyrosine kinase inhibitor (EGFR-TKI) treatment. Mutations in confer sensitivity to EGFR-TKIs patients with advanced non-small cell lung cancer (NSCLC). This study determined concordance rate serum samples and tumour tissue from Chinese NSCLC compared two detection methods: mutant-enriched polymerase chain reaction-based DNA sequencing non-enriched sequencing. The mutation status was...

MicroRNAs (miRNAs) are important regulators involved in diverse physiological and pathological processes including cancer. SUMO (small ubiquitin-like modifier) is a reversible protein modifier. We recently found that SUMOylation of TARBP2 DGCR8 the regulation miRNA pathway. KHSRP single stranded nucleic acid binding with roles transcription mRNA decay, it also component Drosha-DGCR8 complex promoting biogenesis. The vivo assay using Ni2+-NTA affinity pulldown or immunoprecipitation (IP)...

Abstract Small RNA-induced gene silencing is essential for post-transcriptional regulation of expression; however, it remains unclear how miRNA/siRNA efficiency regulated. Here we show that TARBP2 SUMOylated at K52, which can be enhanced by its phosphorylation. This modification stabilize via repressing K 48 -linked ubiquitination. We find SUMOylation does not influence the overall production mature miRNAs, but regulates efficiency. recruits Ago2 to constitute complex (RISC)-loading (RLC),...

FOXM1, a member of the Forkhead transcriptional family, plays an important role in EMT process, and transforming growth factor-β1 (TGF-β1) has been identified as most potent factor that can independently induce various types cancer cells. Here we examine FOXM1 TGF-β1-induced investigate mechanism underlying relationship between TGF-β1 FOXM1. Lentivirus-mediated transfection was used to stably upregulate expression small interfering RNA (siRNA) introduced silence Transwell wound-healing...

Leonurine hydrochloride (LH), a major alkaloid compound extracted from Leonurus japonicas Houtt. (Labiatae), is considered to have antitumor roles.This study investigated its effects on human non-small cell lung cancer (NSCLC) H292 cells and illustrated the possible mechanism involved.After treatment with different concentrations of LH (0, 10, 25, 50 μmol/L) for 6, 12, 24, 48, 72 h, viability was assessed by MTT assay. After exposed doses 24 cell-cycle distribution, apoptosis, mitochondrial...

The purpose of this study is to explore the prognostic value associating pre-treatment neutrophil-lymphocyte ratio (NLR) with circulating tumor cells counts (CTCs) in patients gastrointestinal cancer.We collected related data 72 gastric cancer (GC) and colorectal (CRC) who received different therapies from August 2016 October 2020, including age, gender, primary location, TNM stage, tumor-differentiation, NLR, CTCs, disease-free survival (DFS) overall (OS). We chose optimal cut-off NLR >3.21...

Circulating free DNA (cfDNA) in plasma is promising to be a surrogate for tumor tissue DNA. However, not all epidermal growth factor receptor (EGFR) mutations has been detected matched cfDNA, at least partly due inefficient cfDNA extraction method. The purpose of this study was establish an efficient protocol.The yield extracted by our modified phenol-chloroform (MPC) method from non-small-cell lung cancer (NSCLC) patients compared with that QIAamp MinElute Virus Spin kit (Qiagen kit) as...

// Rong Deng 1 , Xian Zhao YingYing Qu Cheng Chen Changhong Zhu Hailong Zhang Haihua Yuan Hui Jin Xin Liu Yanli Wang Qin Jian Huang Jianxiu Yu 1, 2, 3 Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory Tumor Microenvironment Inflammation, Jiao Tong University School Medicine, 200025, China 2 State Oncogenes Related Genes, Institute Oncology & Oncology, 9th People's Hospital, Correspondence to: Yu, e-mail: Jianxiu.Yu@gmail.com Keywords: Shp2, SUMOylation, Gab1, ERK...

Aim: To explore the prognostic value of systemic inflammatory marker (SIM) based on neutrophil, lymphocyte and monocyte counts in head neck squamous cell carcinoma (HNSCC) patients. Patients & methods: We retrospectively collected data 367 patients with HNSCC who underwent surgery. The Kaplan–Meier survival analysis Cox regression were conducted disease-free overall survival. Results: A high SIM (>1.34) was associated larger tumor size, advanced clinical stage shorter time. showed that only...

Background: To investigate the relationship of circulating tumor cells (CTCs) and clinical characteristic parameters prognosis in patients with head neck squamous cell carcinoma (HNSCC). Methods: The retrospective study included 95 HNSCC who after surgery Shanghai Ninth People’s Hospital affiliated to Jiao Tong University School Medicine between December 2015 2016. All were followed up for survival until end June 2019. CTCs detection was performed by negative enrichment (NE)...

SUMOylation is an important post-translational modification that participates in a variety of cellular physiological and pathological processes eukaryotic cells. Sirt2, NAD+-dependent deacetylase, usually exerts tumor-suppressor function. However, the role cancer cells not fully known. In this study, we found can occur Sirt2 protein at both lysine 183 340 sites. did affect localization or stability but was involved P38-mTORC2-AKT signal transduction via direct deacetylation on new substrate...

Abstract MAPK/p38 is an important mammalian signaling cascade that responds to a variety of intracellular or extracellular stimuli, such as reactive oxygen species (ROS), and participates in numerous physiological pathological processes. However, the biological function p38 different tumors, even at stages same tumor, remains elusive. To further understand regulatory mechanism oxidative stress occurrence development gastric cancer, we report SUMOylation novel post-translational modification...

Lung cancer is one of the most threatening malignant tumors to human health. Epidermal growth factor receptor ( EGFR )‐targeted therapy a common and essential means for clinical treatment lung cancer. However, drug resistance has always affected therapeutic effect survival rate in non‐small cell NSCLC ). Tumor heterogeneity significant reason, yielding various mechanisms, such as ‐dependent or ‐independent extracellular signal‐regulated kinase 1 and/or 2 ERK1 /2) activation . To examine...

Androgen-regulated DNA damage response (DDR) is one of the essential mechanisms in prostate cancer (PCa), a hormone-sensitive disease. The heterogeneous nuclear ribonucleoprotein K (hnRNPK)-homology splicing regulatory protein known as far upstream element-binding 2 (KHSRP) an RNA-binding that can attach to AU-rich elements 3' untranslated region (3'-UTR) messenger RNAs (mRNAs) mediate mRNA decay and emerges critical regulator DDR preserve genome integrity. Nevertheless, how KHSRP responds...