The osteocyte is believed to act as the main sensor of mechanical stimulus in bone, controlling signalling for bone growth and resorption response changes demands placed on our bones throughout life. However, precise stimuli that cells experience vivo are not yet fully understood. objective this study use computational methods predict loading conditions experienced by osteocytes during normal physiological activities. Confocal imaging lacunar–canalicular network was used develop...

Significance Bone fragility increases with age as the result of concurrent decline bone mass, quality, and mechanosensitivity. While coordinated these behaviors remains unexplained, role osteocytes in each processes age-related degeneration lacunocanalicular network (LCN) which they reside implicate aging. In this work, we identify canalicular loss a driver behind declining mass transport mechanostimulation within LCN aged osteocyte-intrinsic defects transforming growth factor beta...

Androgen deprivation therapy (ADT) for localised and metastatic prostate cancer (PCa) is known to improve survival in patients but has been associated with negative long-term impacts on the skeleton, including decreased bone mineral density (BMD) increased fracture risk. Previously, dual-energy x-ray absorptiometry (DXA) measurements of areal BMD (aBMD) vertebrae. However, a prediction vertebral strength requires information that aBMD cannot provide, such as geometry volumetric (vBMD). This...

Fetal movements in the uterus are a natural part of development and known to play an important role normal musculoskeletal development. However, very little is about biomechanical stimuli that arise during utero, despite these being crucial bone joint formation. Therefore, objective this study was create series computational steps by which forces generated kick utero could be predicted from clinically observed fetal using novel cine-MRI data three fetuses, aged 20–22 weeks. A custom tracking...

Mechanical forces generated by fetal kicks and movements result in stimulation of the skeleton form stress strain. This is known to be critical for prenatal musculoskeletal development; indeed, abnormal or absent have been implicated multiple congenital disorders. However, mechanical strain experienced developing human utero never before characterized. Here, we quantify biomechanics during second half gestation modelling captured using novel cine-magnetic resonance imaging technology. By...

Metastatic bone disease is a complex condition resulting from the migration and colonization of cancer cells their primary site to microenvironment, where they typically develop metastatic niche. Osteocytes, most abundant in tissue master regulators remodelling, are increasingly thought play crucial role this process through intricate interactions with cells. This review covers recent progress made exploring multifaceted between osteocytes highlighting importance signalling networks...

Alterations in bone tissue composition during osteoporosis likely disrupt the mechanical environment of cells and may thereby initiate a mechanobiological response. It has proved challenging to characterize vivo, osteoporotic is not known. The objective this research local osteocytes osteoblasts from healthy rat model osteoporosis. Using custom-designed micromechanical loading device, we apply strains representative range physical activity (up 3000 με) fluorescently stained femur samples...

The fetal membrane surrounds the fetus during pregnancy and is a thin tissue composed of two layers, chorion amnion. While rupture this normally occurs at term, preterm can result in increased risk mortality morbidity, as well danger infection mother. Although structural changes have been observed such cases, mechanical behaviour human vivo remains poorly understood challenging to investigate experimentally. Therefore, objective study was develop simplified finite element models membrane,...

Mechanical environment plays a crucial role in regulating bone regeneration defects. Assessing the mechanobiological behavior of patient-specific orthopedic scaffolds in-silico could help guide optimal scaffold designs, as well intra- and post-operative strategies to enhance improve implant longevity. Additively manufactured porous scaffolds, specifically triply periodic minimal surfaces (TPMS), have shown promising structural properties act substitutes, yet their ability induce...

Breast and prostate cancers preferentially metastasise to bone tissue, with metastatic lesions forming in the skeletons of most patients. On arriving disseminated tumour cells enter a mechanical microenvironment that is substantially different primary largely regulated by cells. Osteocytes, ubiquitous cell type, orchestrate healthy remodelling response physical exercise. However, effects loading osteocytes on cancer behaviour still poorly understood. The aim this study was characterise...

Abstract Bone metastases are a common cause of suffering in breast and prostate cancer patients, however, the interaction between bone cells is poorly understood. Using series co‐culture, conditioned media, human spheroid, organ‐on‐a‐chip experiments, this study reveals that osteocytes suppress cell proliferation increase migration via tumor necrosis factor alpha (TNF‐α) secretion. This action regulated by osteocyte primary cilia associated intraflagellar transport protein 88 (IFT88)....

Recent therapeutic advancements have markedly increased the survival rates of individuals with multiple myeloma (MM), doubling compared to pre-2000 estimates. This progress, driven by highly effective novel agents, suggests a growing population MM survivors exceeding 10-year mark post-diagnosis. However, contemporary clinical observations indicate potential trends toward more aggressive relapse phenotypes, characterized extramedullary disease and dominant proliferative clones, despite these...

SUMMARY Bone continuously adapts its internal structure to accommodate the functional demands of mechanical environment. This process is orchestrated by a network mechanosensitive osteocytes that respond external signals and recruit osteoblasts osteoclasts alter bone mass meet loading demands. Because irregular hierarchical microarchitecture tissue, precise stimuli experienced located in different regions tissue not well‐understood. The objective this study characterise local stimulus...

Fetal kicking and movements generate biomechanical stimulation in the fetal skeleton, which is important for prenatal musculoskeletal development, particularly joint shape. Developmental dysplasia of hip (DDH) most common shape abnormality at birth, with many risk factors condition being associated restricted movement. In this study, we investigate biomechanics such situations, namely breech position, oligohydramnios primiparity (firstborn pregnancy). We also twin pregnancies, are not...

The primary cilium is a solitary, sensory organelle with many roles in bone development, maintenance, and function. In the osteogenic cell lineage, including skeletal stem cells, osteoblasts, osteocytes, plays vital role regulation of formation, this has made it promising pharmaceutical target to maintain health. While lineage been increasingly characterized, little known about potential impact targeting relation osteoclasts, hematopoietic responsible for resorption. objective study was...