A5059781025- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Venomous Animal Envenomation and Studies
- Signaling Pathways in Disease
- Sports injuries and prevention
- Healthcare and Venom Research
- Ubiquitin and proteasome pathways
- Sports Performance and Training
- Ion channel regulation and function
- Nerve injury and regeneration
- Heat shock proteins research
- Nutrition and Health in Aging
- Tissue Engineering and Regenerative Medicine
- Pain Mechanisms and Treatments
- Cardiovascular Effects of Exercise
- Neurogenetic and Muscular Disorders Research
- Genetics and Physical Performance
- Laser Applications in Dentistry and Medicine
- Cardiomyopathy and Myosin Studies
- Nitric Oxide and Endothelin Effects
- Histone Deacetylase Inhibitors Research
- Mesenchymal stem cell research
- Cardiovascular Function and Risk Factors
Universidade de São Paulo
2014-2024
Stanford University
2014
Bridge University
2013
Edith Cowan University
2013
Institute of Biomedical Science
2012
Universidade Brasil
2005
Universidade Federal de São Carlos
2001
A Wnt-TGFβ2 signaling pathway modulates stem cell dysfunction in muscles of dystrophic mice.
The aim of this study was to assess the effect leucine supplementation on elements ubiquitin-proteasome system (UPS) in rat skeletal muscle during immobilization. This evaluated by submitting animals a protocol hindlimb immobilization, after which different parameters were determined, including: mass; cross-sectional area (CSA); gene expression E3 ligases/deubiquitinating enzymes; content ubiquitinated proteins; and rate protein synthesis. Our results show that attenuates soleus mass loss...
This study was undertaken in order to provide further insight into the role of leucine supplementation skeletal muscle regeneration process, focusing on myofiber size and strength recovery. Young (2-month-old) rats were subjected or not (1.35 g/kg per day) started 3 days prior cryolesion. Then, soleus muscles cryolesioned continued receiving until 1, 10 later. Soleus from leucine-supplemented animals displayed an increase a reduction collagen type III expression post-cryolesion day 10....
Abstract Aim To identify microRNAs (miRs) involved in the regulation of skeletal muscle mass. For that purpose, we have initially utilized an silico analysis, resulting identification miR‐29c as a positive regulator Methods was electrotransferred to tibialis anterior address its morphometric and functional properties determine level satellite cell proliferation differentiation. qPCR used investigate effect overexpression on trophicity‐related genes. C2C12 cells were impact myogenesis...
Heat shock protein expression is elevated upon exposure to a variety of stresses and limits the extent stress-induced damage. To investigate putative role inducible 70-kDa heat (HSP70) in skeletal muscle damage regeneration, soleus tibialis anterior (TA) muscles from HSP70-overexpressing transgenic mice were subjected cryolesioning analyzed after 1, 10, 21 days. Histological analysis showed that both HSP70 wild-type treated with radicicol (a HSP inducer) had decreased necrosis compared...
Abstract The bone–muscle unit refers to the reciprocal regulation between bone and muscle by mechanical interaction tissue communication via soluble factors. RANKL stimulation induces mitochondrial biogenesis increases oxidative capacity in osteoclasts adipocytes. may bind membrane bound RANK or osteoprotegerin (OPG), a decoy receptor that inhibits RANK–RANKL activation. is highly expressed skeletal muscle, but contribution of healthy fiber remains elusive. Here we show C2C12-derived...
Although we have shown that catecholamines suppress the activity of Ubiquitin-Proteasome System (UPS) and atrophy-related genes expression through a cAMP-dependent manner in skeletal muscle from rodents, underlying mechanisms remain unclear. Here, report single injection norepinephrine (NE; 1 mg kg-1 ; s.c) attenuated fasting-induced up-regulation FoxO-target tibialis anterior (TA) muscles by stimulation PKA/CREB Akt/FoxO1 signaling pathways. In addition, muscle-specific activation PKA...
In this study we investigated the gene expression of proteins related to myostatin (MSTN) signaling during skeletal muscle longitudinal growth. To promote growth, Wistar male rats were submitted a stretching protocol for different durations (12, 24, 48, and 96 hours). Following protocol, soleus weight length sarcomere number determined. addition, levels genes that encode MSTN, follistatin isoforms 288 315 (FLST288 FLST315), follistatin-like 3 protein (FLST-L3), growth differentiation...
Zavanela, PM, Crewther, BT, Lodo, L, Florindo, AA, Miyabara, EH, and Aoki, MS. Health fitness benefits of a resistance training intervention performed in the workplace. J Strength Cond Res 26(3): 811–817, 2012—This study examined effects workplace-based on different health-, fitness-, work-related measures untrained men (bus drivers). The subjects were recruited from bus company divided into (n = 48) control groups after initial prescreening. group 24-week program, whereas maintained their...
In the present study we have compared effects of leucine supplementation and its metabolite β-hydroxy-β-methyl butyrate (HMB) on ubiquitin-proteasome system PI3K/Akt pathway during two distinct atrophic conditions, hindlimb immobilization dexamethasone treatment. Leucine was able to minimize reduction in rat soleus mass driven by immobilization. On other hand, unable provide protection against loss treated rats. Interestingly, HMB all treatments. While solely fiber type I cross sectional...
The neural mobilization (NM) technique is a noninvasive method that has been proven to be clinically effective in reducing pain; however, the molecular mechanisms involved remain poorly understood. aim of this study was analyze whether NM alters expression mu-opioid receptor (MOR), delta-opioid (DOR) and Kappa-opioid (KOR) periaqueductal gray (PAG) improves locomotion muscle force after chronic constriction injury (CCI) rats. CCI imposed on adult male rats followed by 10 sessions every other...
Abstract This work was undertaken to provide further insight into the role of mammalian target rapamycin complex 1 (mTORC1) in skeletal muscle regeneration, focusing on myofiber size recovery. Rats were treated or not with rapamycin, an mTORC1 inhibitor. Soleus muscles then subjected cryolesion and analyzed 1, 10, 21 days later. A decrease soleus cross‐section area post‐cryolesion 10 accentuated by which also effective reducing protein synthesis these freeze‐injured muscles. The incidence...
This study investigated the effect of leucine supplementation on skeletal muscle regenerative process, focusing remodeling connective tissue fast twitch tibialis anterior (TA). Young male Wistar rats were supplemented with (1.35 g/kg per day); then, TA muscles from left hind limb cryolesioned and examined after 10 days. Although induced increased protein synthesis, it was not sufficient to promote an increase in cross-sectional area (CSA) regenerating myofibers (p > 0.05) muscles....
Acute renal failure is a serious complication of human envenoming by Bothrops snakes. The ion pump Na+/K+-ATPase has an important role in tubule function, where it modulates sodium reabsorption and homeostasis the extracellular compartment. Here, we investigated morphological functional alterations changes expression activity rats injected with alternatus snake venom.Male Wistar were venom (0.8 mg/kg, i.v.) function was assessed 6, 24, 48 72 h 7 days post-venom. then killed assayed based on...
β2-adrenergic stimulation causes beneficial effects on structure and function of regenerating muscles; thus, the β2-adrenoceptor may play an important role in muscle regenerative process. Here, we investigated β2 -adrenoceptor skeletal regeneration.Tibialis anterior (TA) muscles from knockout (β2 KO) mice were cryolesioned analysed after 1, 3, 10 21 days. The was assessed through analysis morphological contractile aspects, M1 M2 macrophage profile, cAMP content, activation TGF-β signalling...
In order to gain insight into intracellular mechanisms involved in longitudinal growth of skeletal muscle, we determined gene expression ubiquitin-ligases (MAFbx/atrogin-1, E3 alpha, and MuRF-1) deubiquitinating enzymes (UBP45, UBP69, USP28) at different time-points (24, 48, 96 h) continuous stretch the soleus tibialis anterior (TA) muscles. soleus, real-time polymerase chain reaction (PCR) showed that MAFbx/atrogin-1, MuRF-1 was downregulated, peaking 24-48 h. Gene all increased with...
The present study investigated changes in indirect markers of muscle damage following a simulated tennis match play using nationally ranked young (17.6 ± 1.4 years) male players.Ten athletes played 3-hour on outdoor red clay courts the International Tennis Federation rules.Muscle soreness, plasma creatine kinase activity (CK), serum myoglobin concentration (Mb), one repetition maximum (1RM) squat strength, and jump (SJ) counter movement (CMJ) heights were assessed before, immediately after,...
Abstract Background Fetal stage is a critical developmental window for the skeletal muscle, but little information available about impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in adult life male and female animals. Methods Female rats (Wistar Hannover) were fed either control (1000 IU Vit. D3/kg) or VDD diet (0 6 weeks during gestation lactation. At weaning, randomly separated received standard up to 180 days old. Results Vitamin induced muscle...