Abstract Aphelids are little-known phagotrophic parasites of algae whose life cycle and morphology resemble those the parasitic rozellids (Cryptomycota, Rozellomycota). In previous phylogenetic analyses RNA polymerase rRNA genes, aphelids, Microsporidia (parasites animals) formed a clade, named Opisthosporidia, which appeared as sister group to Fungi. However, statistical support for Opisthosporidia was always moderate. Here, we generated full life-cycle transcriptome data aphelid species...

Ophthalmic complications of 131I therapy, including ocular dryness, have been recently investigated and described. However, nasolacrimal drainage system obstruction (NDSO), complicating has not previously well appreciated or characterized. One our patients developed bilateral complete duct after therapy that prompted awareness this potential complication. Over 16 months, 423 with epithelial-derived thyroid cancer were provided routine clinical care; 390 these had received ablation 10...

OBJECTIVE—To determine the effectiveness of specialist nurse-led clinics for hypertension and hyperlipidemia provided diabetic patients receiving hospital-based care. RESEARCH DESIGN AND METHODS—This study was a randomized controlled implementation trial at Hope Hospital, Salford, U.K. The subjects consisted 1,407 presenting annual review with raised blood pressure (≥140/80 mmHg), total cholesterol (≥5.0 mmol/l), or both. Individuals diabetes were to usual care subsequent invitation attend...

Abstract All animals develop from a single-celled zygote into complex multicellular organism through series of precisely orchestrated processes. Despite the remarkable conservation early embryogenesis across animals, evolutionary origins this process remain elusive. By combining time-resolved imaging and transcriptomic profiling, we show that single cells ichthyosporean Chromosphaera perkinsii - close relative diverged approximately 1 billion years ago undergo symmetry breaking cleavage...

In Brief Purpose To report the finding of nasolacrimal drainage system obstruction associated with I131 therapy for thyroid carcinoma from an updated and expanded cohort. Methods Patients a history epithelial derived who had tearing were offered referral evaluation by oculoplastic surgeon. underwent probing irrigation procedures localization their obstruction. Therapy site was instituted. Results Clinically significant identified in 26 patients, all whom previously undergone (n = 563)....

During embryonic development, cells of the green alga Oophila amblystomatis enter salamander Ambystoma maculatum forming an endosymbiosis. Here, using de novo dual-RNA seq, we compared host that harbored intracellular algae to those without and inside animal in egg capsule. This two-by-two-way analysis revealed exhibit hallmarks cellular stress undergo a striking metabolic shift from oxidative metabolism fermentation. Culturing experiments with showed glutamine may be utilized by algal...

While algal phago-mixotrophs play a major role in aquatic microbial food webs, their diversity remains poorly understood. Recent studies have indicated several species of prasinophytes, early diverging green algae, to be able consume bacteria for nutrition. To further explore the occurrence phago-mixotrophy we conducted feeding experiments with live fluorescently labeled stained CellTracker Green CMFDA, heat-killed 5-(4,6-dichlorotriazin-2-yl) aminofluorescein (DTAF), and magnetic beads....

Protists, defined as unicellular eukaryotes distinct from animals, plants, and fungi, are a polyphyletic group that predominates the eukaryotic tree of life, exhibiting significant phylogenetic diversity fulfilling critical ecological roles. Historically, research has prioritized protists associated with animals particularly those medical significance, thereby overlooking majority protist diversity. Conventional molecular methods, such 18S rRNA gene amplicon sequencing, frequently encounter...

Acute dacryocystitis frequently is extremely painful and slow to resolve even with systemic antibiotic therapy. We have identified that incision, drainage, direct application of antibiotics inside the infected sac result in almost immediate resolution pain rapid control infection. This also provides optimal culture material. Twelve consecutive patients treated this manner had acute infectious process. All eight subsequently undergoing dacryocystorhinostomy were fully cured. A total 58.3%...

In Brief Purpose: Nasolacrimal outflow obstruction has been associated with high-dose (>150 mCi) radioactive iodine (I131) treatment. Commonly used for thyroid cancer treatment, I131 is effectively transported in the targeted tissue by Na+/I symporter (NIS). We hypothesized that NIS expressed lacrimal sac and nasolacrimal duct active accumulation of responsible clinical observations seen these patients Methods: Reverse transcriptase–polymerase chain reaction immunohistochemical analyses were...

• This is, to our knowledge, the first report in ophthalmic literature of punctal-canalicular stenosis and concomitant epiphora associated with prolonged administration fluorouracil; fluorouracil is systemically administered agent cause obstruction lacrimal system. Each three patients receiving this antimetabolite alone one patient it combination methotrexate cyclophosphamide were studied for complaints epiphora. All four exhibited or occlusion more puncta, while also had their canaliculi...

In Brief Purpose: To evaluate the safety and efficacy of botulinum toxin type A for treatment eyelid retraction resulting from thyroid eye disease (TED) during inflammatory phase condition. Methods: this prospective, nonrandomized case series, 18 patients with caused by active TED received injection (10, 5, or 2.5 U) upper retraction. Botulinum (Allergan, Irvine, CA, U.S.A.) was injected transconjunctivally just above superior tarsal border in elevator complex eyelid. Results: Seventeen...

Purpose. To analyze the clinical findings associated with involutional entropion and ectropion compare them to each other age-matched controls. Methods. Prospective, cohort study involving 30 lids entropion, ectropion, 52 control lids. Results. The statistically significant differences both groups compared group were presence of a retractor dehiscence, “white line,” occurrence orbital fat prolapse in cul-de-sac, decreased lower lid excursion, increased laxity by snapback test, an...

Cymbomonas tetramitiformis—a marine prasinophyte—is one of only a few green algae that still retain an ancestral particulate-feeding mechanism while harvesting energy through photosynthesis. The genome the alga is estimated to be 850 Mb–1.2 Gb in size—the bulk which filled with repetitive sequences—and annotated 37,366 protein-coding gene models. A number unusual metabolic pathways (for Chloroplastida) are predicted for C. tetramitiformis, including Lipid-A and peptidoglycan metabolism....

Placozoa is an ancient phylum of extraordinarily unusual animals: miniscule, ameboid creatures that lack most fundamental animal features. Despite high genetic diversity, only recently have the second and third species been named. While prior genomic studies suffer from incomplete placozoan taxon sampling, we more than double count with protein sequences seven key genomes produce first nuclear phylogenomic reconstruction all major lineages. This leads us to complete Linnaean taxonomic...

Revolutionary advancements in underwater imaging, robotics, and genomic sequencing have reshaped marine exploration. We present demonstrate an interdisciplinary approach that uses emerging quantitative imaging technologies, innovative robotic encapsulation system with situ RNA preservation next-generation to gain comprehensive biological, biophysical, data from deep-sea organisms. The synthesis of these provides rich morphological genetic information for species description, surpassing...

All animals develop from a single-celled zygote into complex multicellular organism through series of precisely orchestrated processes1,2. Despite the remarkable conservation early embryogenesis across animals, evolutionary origins how and when this process first emerged remain elusive. Here, by combining time-resolved imaging transcriptomic profiling, we show that single cells ichthyosporean Chromosphaera perkinsii—a close relative diverged about 1 billion years ago3,4—undergo symmetry...

Upper eyelid position, pupillary diameter, and visual field impairment were measured in 26 eyes with adult-onset blepharoptosis. The magnitude of superior was inversely proportional to the distance between upper lid margin aperture. central corneal reflex most useful measurement for predicting impairment. Superior present when <2.5 mm. Visual inferiorly, temporally, nasally occurred obscured <1.5 mm vertical diameter pupil visible. Observation relationship aperture provides information...

O6-Methylguanine (O6-meG), which is produced in DNA following exposure to methylating agents, instructs human RNA polymerase II mis-insert bases opposite the lesion during transcription. In this study, we examined effect of O6-meG on transcription cells and investigated subsequent effects protein function translation resulting mRNA. HEK293 cells, induced incorporation uridine or cytidine nascent adduct. containing active O6-alkylguanine-DNA alkyltransferase (AGT), repairs O6-meG, 3%...

The focus of this paper is on the quality historical text digitised through optical character recognition (OCR) and how it affects mining. We study effect OCR errors have named entity (NER) show that in a random sample documents picked from several collections, 30.6% false negative commodity location mentions 13.3% all manually annotated contain errors. introduce simple method for estimating OCRed examine well human raters can evaluate it. also illustrate automatic estimation compares to...

Summary Phytomyxea are intracellular biotrophic parasites infecting plants and stramenopiles, including the agriculturally impactful Plasmodiophora brassicae brown seaweed pathogen Maullinia ectocarpii . They belong to clade Rhizaria, where phagotrophy is main mode of nutrition. Phagocytosis a complex trait eukaryotes, well documented for free‐living unicellular eukaryotes specific cellular types animals. Data on phagocytosis in intracellular, scant. Phagocytosis, parts host cell consumed at...