A5074154425- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Sirtuins and Resveratrol in Medicine
- Immune Response and Inflammation
- interferon and immune responses
- Multiple Myeloma Research and Treatments
- FOXO transcription factor regulation
- Genetics and Neurodevelopmental Disorders
- Immune cells in cancer
- Heat shock proteins research
- Neuroblastoma Research and Treatments
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- PARP inhibition in cancer therapy
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Genomics and Chromatin Dynamics
- vaccines and immunoinformatics approaches
Fred Hutch Cancer Center
2022-2024
Sana Biotechnology (United States)
2022-2023
University of Massachusetts Chan Medical School
2015
Seattle University
2009-2012
Center for Infectious Disease Research
2012
Institute for Systems Biology
2007-2010
Abstract Although TLR5 regulates the innate immune response to bacterial flagellin, it is unclear whether its function essential during in vivo murine infections. To examine this question, we challenged Tlr5−/− mice transurethrally with Escherichia coli. At 2 days postinfection, wild-type exhibited increased inflammation of bladder comparison mice. By day 5 had significantly more bacteria bladders and kidneys showed both organs. In addition, flagellin induced high levels cytokine chemokine...
Abstract Motivation: Histone acetylation (HAc) is associated with open chromatin, and HAc has been shown to facilitate transcription factor (TF) binding in mammalian cells. In the innate immune system context, epigenetic studies strongly implicate transcriptional response of activated macrophages. We hypothesized that using data from large-scale sequencing a chromatin immunoprecipitation assay (ChIP-Seq) would improve performance computational prediction locations TFs mediating signaling...
Intrahepatic cholangiocarcinoma (ICC) is an aggressive bile duct malignancy that frequently exhibits isocitrate dehydrogenase (
Pancreatic ductal adenocarcinoma (PDAC) is classified into two key subtypes, classical and basal, with basal PDAC predicting worse survival. Using in vitro drug assays, genetic manipulation experiments, vivo studies human patient-derived xenografts (PDXs) of PDAC, we found that PDACs were uniquely sensitive to transcriptional inhibition by targeting cyclin-dependent kinase 7 (CDK7) CDK9, this sensitivity was recapitulated the subtype breast cancer. We showed cell lines, PDXs, publicly...
Abstract Introduction: Fusosomes are novel viral vectors pseudotyped with a modified paramyxovirus envelope targeting specific cell types. We have generated CD8-targeted fusosome to deliver CD19-directed chimeric antigen receptor (CAR) transgene CD8+ T cells in vivo following intravenous (i.v.) injection. Although fusosomes capable of genetically modifying resting cells, modulation chemical or biological agents may increase transduction and facilitate CAR expansion. Here, we demonstrate that...
Abstract Background: We have developed a novel gene therapy platform based on fusogen biology that allows targeted delivery of CAR transgenes to “resting” T cells through systemic administration CD8-targeted paramyxovirus-based viral vector (VV). Therapies utilizing autologous demonstrated success using the murine CD19-specific scFv, FMC63. These sequences potential incite cell and antibody responses against CAR, which may play role in elimination. This scenario increases likelihood context...
Abstract Pancreatic Ductal Adenocarcinoma (PDA) can be characterized by two distinct transcriptional subtypes: Classical and Basal-like. The basal PDA subtype is more aggressive has the worst overall survival. Previous work shown that Sirtuin 6 histone deacetylase (SIRT6) acts as a tumor suppressor cooperates with oncogenic KRAS in GEMM models of PDA. Our study identifies SIRT6 biomarker for investigates underlying sensitivity to inhibition cyclin dependent kinases (CDKs). Through analysis...