Cells of Escherichia coli under conditions certain cellular stresses excrete attractants. chemotactic strains respond to these excreted signaling molecules by moving up their local concentration gradients and forming different types stable multicellular structures. Multicellular clusters are the simplest among Fluorescence microscopy was used characterize macroscopic properties track individual E. cells in real time. A quantitative analysis reveals that equilibrium cluster size is only...

We present a novel technique to accurately position single cells on substrate using negative dielectrophoresis and cell–substrate adhesion. The are suspended in physiological media throughout the patterning process. also verify biocompatibility of this method by demonstrating that patterned proliferate show normal morphology. calculate temperatures transmembrane potential device experience compare them physiologically acceptable levels described previous studies.

The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches the in vitro study human cardiac physiology and pathophysiology. We present a method to comprehensively assess single pluripotent cell-derived (hPSC-CMs) through simultaneous contraction kinetics, force generation, electrical activity. demonstrate that statistical movies contracting hPSC-CMs can be used quantify changes cellular morphology over time compute contractile kinetics. Using...

In mammals, cardiac development proceeds from the formation of linear heart tube, through complex looping and septation, all while increasing in mass to provide oxygen delivery demands embryonic growth. The developing must orchestrate regional differences cardiomyocyte proliferation control morphogenesis. During ventricular wall formation, compact myocardium proliferates more vigorously than trabecular myocardium, but mechanisms controlling such among populations are not understood. Control...

Abstract In this work, the performance of N‐(3‐trimethoxysilylpropyl)diethylenetriamine (DAEAPTS)‐grafted mesoporous SBA‐15 adsorbent for postcombustion carbon capture was studied. Wet grafting technique adopted to functionalize surface. The adsorption CO 2 on amine‐grafted sorbent measured by thermogravimetric method over a partial pressure range 0–90 kPa and temperature 25–105°C under atmospheric pressure. optimal amine loaded containing 40 wt% DAEAPTS exhibited capacity up 2.3 mmol/g...

Pluripotent stem cell derived hepatocyte-like cells (hPSC-HLCs) are an attractive alternative to primary human hepatocytes (PHHs) used in applications ranging from therapeutics drug safety testing studies. It would be critical improve and maintain mature hepatocyte functions of the hPSC-HLCs, especially for long-term If 3D culture systems were such purposes, it important that system can support formation maintenance optimal-sized spheroids long periods time, also directly deployed liver...

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency monoclonal antibodies (mAbs) and identify viral escape mutants neutralization severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on use execution this protocol, please refer Zost et al. (2020) Suryadevara (2021).

Obtaining functional hepatocytes from human pluripotent stem cells (hPSCs) holds great potential for applications in drug safety testing, as well the field of regenerative medicine. However, developing functionally mature hPSC-derived (hPSC-Heps) remains a challenge. We hypothesized that cellular microenvironment plays vital role maturation immature hepatocytes. In this study, we examined mechanical stiffness, key component microenvironment, hPSC-Heps. cultured hPSC-Heps on collagen-coated...

Defining the pathways that control cardiac development facilitates understanding pathogenesis of congenital heart disease. Herein, we identify enrichment a Cullin5 Ub ligase key subunit, Asb2, in myocardial progenitors and differentiated cardiomyocytes. Using two conditional murine knockouts, Nkx+/Cre.Asb2fl/fl AHF-Cre.Asb2fl/fl, tissue clarifying technique, reveal Asb2 requirement for embryonic survival complete looping. Deletion results upregulation its target Filamin A (Flna), concurrent...

A common assumption made when performing in vitro cellular assays is that the concentration of substances culture system uniform. However, since cells internalize and secrete reside at bottom well, it conceivable a gradient could arise across fluid layer. Importantly, substance vicinity cell, which interest, cannot be measured via existing methods. In this work simple strategy for estimating chemical species surface cell presented. Finally, result used to outline method determining...

Abstract Rhabdomyosarcoma (RMS) is the most common childhood soft tissue sarcoma, with two subtypes: Fusion-positive RMS (FPRMS), which has PAX3-FOXO1 fusion gene, and fusion-negative (FNRMS). Despite their distinct characteristics, treatments mainly rely on conventional chemotherapies without considering these differences. This study highlights that FNRMS cells exhibit significantly heightened interaction extracellular matrix (ECM) compared to FPRMS cells. Using single-cell RNA sequencing...

Cell-cell interactions consisting of diffusible signaling and cell-cell contact (juxtacrine signaling) are important in numerous biological processes such as tumor growth, stem cell differentiation, self-renewal. A number methods currently exist to modulate vitro. One method modulating the total amount is vary seeding density during culture. Due random nature seeding, this results considerable variation actual spacing contact, cannot prescribe local environment. more specific approach for...

ABSTRACT Emerging novel human contagious viruses and pathogens put humans at risk of hospitalization possibly death due to the unavailability vaccines drugs which may take years develop. Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic theWorld Health Organization has over 550,000 deaths worldwide July 2020. Accurate scalable point-of-care devices would increase screening, diagnosis, monitoringof COVID-19...

Abstract Previous studies have suggested that primary hepatocytes cultured in vitro undergo a rapid loss of function. On the other hand, clinic, drug induced liver injury typically manifests 5 days to 3 months after starting medication. Thus, novel approaches can maintain function human for longer durations time may enable development improved assays detecting hepatotoxicity. demonstrated two-dimensional micro-patterning with fibroblasts leads maintenance hepatocyte phenotype relative...

Abstract Cardiac myocytes (CMs) obtained by differentiating embryonic stem cells (ES-CMs) have an immature phenotype and promoting the maturation of such PSC-derived cardiomyocytes remains a major limitation in development cell models human cardiovascular disease. We cultured murine ES-CMs collagen gel (3D) at low density, or on collagen-coated polystyrene (2D) found that 3D culture results dramatic improvement rate end-state gene expression ES-CMs. There are two main differences between CMs...