A5085734930- Mass Spectrometry Techniques and Applications
- Geological and Geochemical Analysis
- Analytical Chemistry and Chromatography
- Geochemistry and Geologic Mapping
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Computational Drug Discovery Methods
- Geoscience and Mining Technology
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Geomechanics and Mining Engineering
- Nanoplatforms for cancer theranostics
- Pharmacological Effects of Natural Compounds
- Advanced biosensing and bioanalysis techniques
- Ion-surface interactions and analysis
- RNA Interference and Gene Delivery
- Geochemistry and Geochronology of Asian Mineral Deposits
- Geological Studies and Exploration
- Geophysical Methods and Applications
- Crystallization and Solubility Studies
- High-pressure geophysics and materials
- X-ray Diffraction in Crystallography
- Geochemistry and Elemental Analysis
- Receptor Mechanisms and Signaling
Nankai University
2021-2025
Jilin University
2001-2025
Unité Matériaux et Transformations
2022-2025
China Coal Technology and Engineering Group Corp (China)
2013-2024
State Key Laboratory of Medicinal Chemical Biology
2023-2024
University of Wisconsin–Madison
2018-2024
Ministry of Natural Resources
2021-2024
Nanjing Medical University
2024
Beijing University of Chinese Medicine
2003-2023
University of Alberta
2019-2022
Abstract Lysine l -lactylation (K -la ) is a novel protein posttranslational modification (PTM) driven by -lactate. This PTM has three isomers: K , N -ε-(carboxyethyl)-lysine ce and d -lactyl-lysine ), which are often confused in the context of Warburg effect nuclear presence. Here we introduce two methods to differentiate these chemical derivatization high-performance liquid chromatography analysis for efficient separation, isomer-specific antibodies high-selectivity identification. We...
Glycosylation is a major protein post-translational modification whose dysregulation has been associated with many diseases. Herein, an on-tissue chemical derivatization strategy based on positively charged hydrazine reagent (Girard's P) coupled matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed for analysis of N-glycans from FFPE treated tissue sections. The performance the proposed approach evaluated by monosaccharides, oligosaccharides,...
Chrysin is a natural flavonoid compound that has antioxidant and neuroprotective effects. Cerebral ischemia reperfusion (CIR) closely connected with increased oxidative stress in the hippocampal CA1 region homeostasis disorder of transition elements such as iron (Fe), copper (Cu) zinc (Zn). This exploration was conducted to elucidate effects chrysin based on transient middle cerebral artery occlusion (tMCAO) rats. Experimentally, sham group, model (50.0 mg/kg) Ginaton (21.6 Dimethyloxallyl...
Abstract Self‐assembly in living cells represents one versatile strategy for drug delivery; however, it suffers from the limited precision and efficiency. Inspired by viral traits, we here report a cascade targeting‐hydrolysis‐transformation (THT) assembly of glycosylated peptides holistically resembling infection efficient cargo delivery combined tumor therapy. We design peptide via incorporating β‐galactose‐serine residue into bola‐amphiphilic sequences. Co‐assembling with two counterparts...
Tyrosine kinase and phosphatase are two important, antagonistic enzymes in organisms. Development of noninvasive approach for sensing their activity with high spatial temporal resolution remains challenging. Herein, we rationally designed a hydrogelator Nap-Phe-Phe(CF3)-Glu-Tyr-Ile-OH (1a) whose supramolecular hydrogel (i.e., Gel 1a) can be subjected to tyrosine kinase-directed disassembly, its phosphate precursor Nap-Phe-Phe(CF3)-Glu-Tyr(H2PO3)-Ile-OH (1b), which alkaline (ALP)-instructed...
CBT-Cys click condensation reaction has a high second-order rate constant and found wide applicability in recent years.
Tetrathiomolybdate (TM) is used in the clinic for treatment of Wilson's disease by targeting cellular copper efflux protein ATP7B (WLN). Interestingly, both TM and WLN are associated with efficacy cisplatin, a widely anticancer drug. Herein, we show that induces dimerization metal-binding domain (WLN4) through unique sulfur-bridged Mo2S6O2 cluster. expels ions from Cu-WLN4 forms copper-free dimer. The binding Mo to cysteine residues WLN4 inhibits platination protein. Reaction multi-domain...
Abstract Despite extensive efforts on probing the mechanism of Alzheimer’s disease (AD) and enormous investments into AD drug development, lack effective disease-modifying therapeutics complexity pathogenesis process suggest a great need for further insights alternative targets. Herein, we focus chiral effects truncated amyloid beta (Aβ) offer structural molecular evidence epitope region-specific, chirality-regulated Aβ fragment self-assembly its potential impact receptor-recognition. A...
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has emerged as a label-free analytical tool for fast biomolecule profiling on tissue sections. Among various functional molecules, mapping neurotransmitters and related metabolites is of tremendous significance, these compounds are critical to signaling in the central nervous system. Here, we demonstrated use both derivatization reaction-free approaches that greatly reduced signal complexity thus enabled...
Investigating the connection between reactive oxygen species (ROS) and oxidative protein unfolding is critical to reveal mechanisms underlying disease involving elevated ROS misfolding. This could inform development of therapeutics targeting cells based on their redox status. In this study, we developed a plasma-droplet fusion-mass spectrometry platform rapidly assess resilience ROS. home-built system fuses generated from microplasma source with microdroplets tunable nanospray source. At...
Photothermal therapy (PTT) has emerged as a noninvasive and precise cancer treatment modality known for its high selectivity lack of drug resistance. However, the clinical translation many PTT agents is hindered by limited biodegradability inorganic nanoparticles instability organic dyes. In this study, peptide conjugate, IR820-Cys-Trp-Glu-Trp-Thr-Trp-Tyr (IR820-C), was designed to self-assemble into both potent vascular disruption in melanoma treatment. When co-assembled with poorly soluble...
Sialylation, a crucial yet labile protein modification, is increasingly recognized for its role in modulating structure, function, and stability. While the impact of oxidative stress on integrity well-established, protective sialylation against such damage remains poorly understood. This study employs microscale low-temperature plasma device to generate controlled, deep radical oxidation environment mimicking cellular stress. By subjecting free sialic acids (Neu5Ac Neu5Gc) time-resolved...
The Jiamusi Massif in the eastern margin of Eurasia, located at tectonic transition belt between Paleo-Asian Ocean and Paleo-Pacific domain, underwent a pivotal from passive to active continental during late Paleozoic. However, exact timing geodynamics this remain controversial. To address issue, paper presents new petrological, mineralogical, detrital zircon U-Pb geochronology, trace element analyses sedimentary rocks sourced both Heitai Zhenzishan formations Massif. results indicate that...
Protein asymmetry, while crucial for life, can arise from subtle stereoisomerization. However, a comprehensive understanding of the breadth and specificity whole stereoproteome (STEP) has been hindered by insufficient stereoisomeric resolution. Here, we introduce an untargeted, de novo STEP discovery protocol profiling relative quantification. This method employs multidimensional isomeric separation, advanced algorithms, stereoisomer-specific retention time shifts. mapping identifies 182...
Double-stranded RNA (dsRNA) binding proteins (dsRBPs) play crucial roles in various cellular processes, especially the innate immune response. Comprehensive characterization of dsRBPs is essential to understand intricate mechanisms for dsRNA sensing and Traditional methods have predominantly relied on affinity purification, favoring isolation strong binders. Here, we adopt proteome integral solubility alteration (PISA) workflow characterizing dsRBPs, resulting observation 18 known...
Rationally coupling natural biochemical reactions for live-cell synthesis of inorganic nanocrystals with fluorescence, such as quantum dots (QDs) especially near-infrared (NIR), holds significant potential in situ labeling and bioimaging. However, the introduced exogenous reactants intracellularly produced species, e.g., reactive oxygen species (ROS), often cause cell damage, decreasing fluorescence QDs. Herein, we have found that cell-adaptable selenocystine ((Cys-Se)2) can be reduced to...
In situ living cell protein analysis would enable the structural identification and functional interrogation of intracellular proteins in native cellular environments. Previously, we have presented an mass spectrometry (MS) strategy to identify protein/metal ion complex with relatively small molecular weight (Anal. Chem. 2016, 88, 10860–10866). However, it is still challenging directly larger protein/ligand complexes cell, due numerous nonspecific bindings ligands, solvents, other...