It is well established that 14-3-3 proteins are key regulators of multiple stress signal transduction cascades. However, the biological functions soybean proteins, especially in plant drought response, not yet known. In this study, we characterized a Glycine soja gene, GsGF14o, which involved development and response. GsGF14o expression was greatly induced by stress, as evidenced quantitative real-time PCR β-glucuronidase (GUS) activity analysis. overexpression Arabidopsis thaliana resulted...

Rice, being the most important food crop in China, is also world's largest producer and consumer, accounting for 30% of global production consumption. In 2023, brown stripes were observed on leaf sheaths diseased rice seedlings a Jingxi field (22°51'N, 105°56'E) Guangxi, China. The investigated area fields, which was about 1.5 hectares, had an estimated disease incidence 15 ± 5%. Leaf samples exhibiting symptoms suspected stripe collected stored at 4 °C bacterial isolation. junction margins...

Abstract In order to investigate the effects of N deposition on soil biochemistry in secondary forests, one addition experiment was conducted a evergreen broad-leaved forest western edge Sichuan Basin, with highest level background (about 95 kg ha −1 yr ) China. Three treatment levels (+0, +50, +150 were monthly added surface this beginning April 2013. Soil and root biomass 0–10 cm horizon measured from May 2014 2015. respiration for two years (September 2013 August 2015). It showed that...

Heterodera avenae is one of the most important plant pathogens and causes vast losses in cereal crops. As a sedentary endoparasitic nematode, H. secretes effectors that modify defenses promote its biotrophic infection hosts. However, number involved interaction between host remains unclear. Here, we report identification putative regulate on large scale. Our results showed 78 95 suppressed programmed cell death (PCD) triggered by BAX 7 themselves caused Nicotiana benthamiana. Among...

Rabbit can produce meat, fur and leather, serves as an important biomedical animal model. Understanding the microbial community of rabbits helps to raise healthily better support their application models.In this study, we selected 4 healthy Belgium gray collect samples from 12 body sites, including skin, lung, uterus, mouth, stomach, duodenum, ileum, jejunum, colon, cecum, cecal appendix rectum. The microbiota across rabbit whole was investigated via 16S rRNA gene amplicon sequencing. After...

Plant parasitic nematodes secrete effectors into host plant tissues to facilitate parasitism. In this study we identified a G16B09-like effector protein family from the transcriptome of Heterodera avenae, and then verified that most members could suppress programmed cell death triggered by BAX in Nicotiana benthamiana. Ha18764, homologous G16B09, was further characterized for its function. Our experimental evidence suggested Ha18764 specifically expressed dorsal gland dramatically...

Heterodera avenae mainly infects cereal crops and causes severe economic losses. Many studies have shown that parasitic nematodes can secrete effector proteins to suppress plant immune responses then promote parasitism. In this study, we showed HaGland5, a novel of H. avenae, was exclusively expressed in dorsal esophageal gland cell nematode, up-regulated the early stage. Transgenic Arabidopsis thaliana lines expressing HaGland5 were significantly more susceptible schachtii than wild-type...

Rabbit coccidiosis is a major disease caused by various Eimeria species and causes enormous economic losses to the rabbit industry. Coccidia infection has wide impact on gut microbiota intestinal biochemical equilibrium. In present study, we established model of intestinalis in rabbits evaluate jejunal fecal metabolite profiles. Rabbits infected group were orally inoculated with 3 × 103 E. oocysts. On eighth day infection, contents feces collected for 16S rRNA gene sequencing liquid...

Acidovorax citrulli (Ac) is the causal agent of bacterial fruit blotch (BFB), and BFB poses a threat to global watermelon production. Despite its economic importance, molecular mechanisms underlying Ac pathogenicity virulence are not well understood, particularly with regard type III secreted effectors. We identify new effector, AopP, in confirm secretion translocation. AopP suppresses reactive oxygen species burst salicylic acid (SA) content significantly contributes virulence....

Bacterial fruit blotch (BFB), caused by Acidovorax citrulli, seriously affects watermelon and other cucurbit crops, resulting in significant economic losses. However, the pathogenicity mechanism of A. citrulli is not well understood. Plant pathogenic bacteria often suppress plant immune response secreting effector proteins. Thus, identifying proteins determining their functions may improve our understanding underlying pathogenetic mechanisms. In this study, a novel effector, AopN, which...

The nitrate removal rate of denitrifying granular sludge in an upflow blanket reactor is very high and reaches up to 3.6 gNO3-N-gVSS-1 d-1 at a loading (NLR) 32.0 gNO3-N L-1 d-1. However, the exhibits flotation under NLR conditions, where granules become large size (of approximately 3-5 mm), light, easily adhere gas bubbles. In order decrease potentiality sludge, three measures were taken as follows: reducing granules, increasing density weakening adhesion effect While, these did not...

Pasteurized milk contains complex microbial communities affected by sterilization and storage conditions. This microflora may be the possible reason that pasteurized dairy products are highly prone to spoilage. In this study, packaged collected from processing factories in China were stored at 0, 4, 10, 15, 25 ºC for 0−15 days subjected identification using high-throughput sequencing. Accordingly, 6 phyla 44 genera identified as dominant microbiota. Moreover, changes nutritional composition...

Introduction The intestinal structure is the foundation for various activities and functions in poultry. An important question concerns changes status under endotoxin stimulation. This study aimed to investigate mechanism of injury induced by lipopolysaccharide (LPS) Wahui pigeons. Methods Thirty-six 28-day-old healthy pigeons were randomly divided into two groups. experimental group was injected with LPS (100 μg/kg) once per day five days, control treated same amount sterile saline. Blood...

A pot experiment was conducted to study the effect of decomposing Cinnamomum septentrionale leaf litter on growth maize. In this study, morphological traits maize were significantly inhibited when amount reached or exceeded 40 g per pot; Furthermore, during early stage with a large addition, pigment contents by C. litter. Gas chromatography-mass spectrometry used determine volatile substances and 34 compounds identified, several which reported be phytotoxic. conclusion, showed strong...

American ginseng ( Panax quinquefolius L.) is a perennial medicinal plant that has long usage history in China. However, root rot, which mainly caused by Fusarium solani can severely reduce the yield and quality of ginseng, but no disease-resistant variety exists, resistance against this disease not yet well understood. Thus, it very urgent to analyze interaction mechanism regulating interactions between F. mine genes. Using transcriptome data quantitative polymerase chain reaction (qPCR),...

Copperleaf (Acalypha australis; Euphorbiaceae), widely cultivated in China, is a traditional Chinese herbal medicine that used for clearing heat and detoxifying, astringency hemostasis (Zhang Zhang 1994). In September 2021, wild Asian copperleaf plants showed leaf yellowing corner outside greenhouse (22°50' N; 108°17' E), Guangxi Province, China. Galls egg masses were observed on the plant roots approximately 60% of plants. Females second-stage juveniles (J2) dissected extracted from with...

Root-knot nematode disease severely affects the yield and quality of mandarin variety

Luffa cylindrica (L.), an annual climbing herb of the Cucurbitaceae family, has a long planting history in China and is deeply loved by people due to its nutritional medicinal value (Partap S, Kumar A et al., 2012). April 2023, symptoms such as plant dwarfism, decreased yield serious root-knots appeared on L. sampled from commercial production base Beihai, Guangxi, (GPS 21°55′79″ N;109°49′61″ E). The investigated area was about 1.3 ha, incidence root-knot nematode disease almost 90%. roots...