A5029578308- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Synthesis and characterization of novel inorganic/organometallic compounds
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Synthetic Organic Chemistry Methods
- Enzyme Catalysis and Immobilization
- Synthesis and Reactions of Organic Compounds
- Advanced Polymer Synthesis and Characterization
- biodegradable polymer synthesis and properties
- Synthesis and properties of polymers
- CRISPR and Genetic Engineering
- Coordination Chemistry and Organometallics
- Bacterial Genetics and Biotechnology
- Genomics, phytochemicals, and oxidative stress
- Innovative Microfluidic and Catalytic Techniques Innovation
- Synthesis of heterocyclic compounds
- Free Radicals and Antioxidants
- Crystallization and Solubility Studies
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and biological activity
- Organoboron and organosilicon chemistry
Agency for Science, Technology and Research
2015-2025
Environment Agency
2025
Institute for Sustainability
2023-2025
Institute of Chemical and Engineering Sciences
2018-2022
Nanyang Technological University
2010-2016
Abstract Natural products possess significant therapeutic potential but remain underutilized despite advances in genomics and bioinformatics. While there are approaches to activate upregulate natural product biosynthesis both native heterologous microbial strains, a comprehensive strategy elicit production of as well generalizable efficient method interrogate diverse strains collection, remains lacking. Here, we explore flexible robust integrase-mediated multi-pronged activation approach...
Abstract Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis novel doubly glycosylated 24‐membered polyene macrolactam from silent cluster in Streptomyces roseosporus by using CRISPR‐Cas9 activation strategy. Structural characterization this polyketide, named auroramycin, revealed rare isobutyrylmalonyl extender unit unique pair amino sugars. Relative absolute stereochemistry were...
Natural products (NP) are instrumental in drug development, but their discovery and validation remain challenging laborious despite advances both genomic analytical technologies. In this study, we demonstrate the use of an integrated multi-modal characterization a microbial strain library for enhanced natural product discovery. This utilizes language- transformer-based models, through cross-validate rank approach to search mass spectrometry (MS)-genome dataset with high confidence. MS data...
Biocatalysis using whole cell biotransformation presents an alternative approach to producing complex molecules when compared traditional synthetic chemical processes. This method offers several advantages, including scalability, self-contained co-factor recycling systems, the use of cost-effective raw materials, and reduced purification costs. Notably, microbial consortia provides benefits over monocultures by enhancing biosynthesis efficiency productivity through division labor a reduction...
A 1,4-addition of a dichlorogermylene dioxane complex with α,β-unsaturated imine 1 gave dichlorogermane derivative 2 bearing GeC₃N five-membered ring skeleton. By reducing KC₈, cyclic (alkyl)(amino)germylene 3 was synthesized and fully characterized. Germylene readily reacted TEMPO, N₂O S₈, producing the 1:2 adduct 4, oxo-bridged dimer 5 sulfido-bridged 6, respectively.
Abstract Application of the well‐characterized Streptococcus pyogenes CRISPR‐Cas9 system in actinomycetes streptomycetes has enabled high‐efficiency multiplex genome editing and CRISPRi‐mediated transcriptional regulation these prolific bioactive metabolite producers. Nonetheless, SpCas9 its limitations can be ineffective depending on strains target sites. Here, we built tested alternative CRISPR‐Cas constructs based standalone pCRISPomyces‐2 plasmid. We showed that thermophilus CRISPR1 Cas9...
Nature has provided unique molecular scaffolds for applications including therapeutics, agriculture, and food. Due to differences in ecological environments laboratory conditions, engineering is often necessary uncover utilize the chemical diversity. Although we can efficiently activate mine these complex 3D molecules, sufficient production of target molecules further application remain a considerable bottleneck. An example bioactive armeniaspirols, which are potent polyketide antibiotics...
Abstract The first systematic study of the thermal rearrangement/fragmentation 5,5‐disubstituted 1,4,2‐oxathiazoles into isothiocyanates is reported. Structure–activity relationships reveal that choice substituent at 5‐position predominant factor to influence ease fragmentation.
Abstract The rearrangement of substituted 1,4,2‐oxathiazoles to nitriles in the presence water is described. Preliminary investigations suggest that reaction pathway proceeds via a 1,4,2‐oxathiazolium intermediate, followed by trapping with and subsequent decomposition products.
Using an established CRISPR-Cas mediated genome editing technique for streptomycetes, we explored the combinatorial biosynthesis potential of auroramycin biosynthetic gene cluster in Streptomyces roseosporous. Auroramycin is a potent anti-MRSA polyene macrolactam. In addition, has antifungal activities, which unique among structurally similar macrolactams, such as incednine and silvalactam. this work, employed different engineering strategies to target glycosylation acylation machineries...
Two methodologies have been tested for the functionalization of phosphirenes. In first one, C−Si bond a 2-silylphosphirene is activated by substoichiometric quantity fluoride ion (TBAF) in THF at −78 °C. Using this technique, it possible to perform protodesilylation or benzaldehyde. However, room temperature with stoichiometry fluoride, nucleophilic attack takes place P, leading ring-opened fluorophosphine. Stille cross-coupling 2-stannylphosphirene presence [PdL2] as catalyst leads an...
Abstract Recently, de novo MS/MS peptide sequencing has enabled the application of affinity selections to synthetic mixtures that approach diversity phage libraries (> 10 8 random peptides). In conjunction with ‘split-mix’ solid phase synthesis access equimolar mixtures, this provides a straightforward means examine considerably higher than been feasible historically. Here, we offer critical perspective on work, report emerging data, and highlight opportunities for further methods...
Extending the durability of polymer plastics and minimizing toxicity concerns to humans environment are among essential goals toward sustainability. Continuing our efforts in this area, we report herein design, optimized synthesis, migration studies macromolecular benzophenones (MBPs) using three important building blocks including poly(ethylene-co-acrylic acid), pentaerythritol, tris(2,3-epoxypropyl)isocyanurate for sustainable food packaging applications. All MBPs synthesized gram scales,...
Abstract Compared to traditional synthetic chemical processes, biocatalysts offer a more sustainable and eco-friendly approach producing complex molecules. In particular, whole-cell boast numerous advantages, including scalable, self-containing co-factor recycling systems, the use of cost-effective raw materials, reduced purification costs. However, challenges arise when working with microbial consortia for biotransformation cascades. Our encapsulation strategy addresses these by controlling...
Herein we describe an improved synthesis of glucosinolates, in which the quantity and cost materials have been reduced by approximately order magnitude compared to typical literature procedures. This allowed us produce multiple glucosinolates 10–25 gram batches using vessel sizes no larger than 0.5 litres.
A "chemical linearization" approach was applied to synthetic peptide macrocycles enable their de novo sequencing from mixtures using nanoliquid chromatography-tandem mass spectrometry (nLC-MS/MS). This approach─previously individual but not mixtures─involves cleavage of the backbone at a defined position give product capable generating sequence-determining fragment ions. Here, we first established compatibility by Edman degradation with prominent macrocycle scaffold based on
Application of the well-characterized Streptococcus pyogenes CRISPR-Cas9 system in actinomycetes has enabled high efficiency multiplex genome editing and CRISPRi-mediated transcriptional regulation these prolific bioactive metabolite producers. Nonetheless, SpCas9 its limitations can be ineffective depending on strains target sites. Here, we built tested alternative CRISPR-Cas constructs based standalone pCRISPomyces-2 plasmid. We showed that thermophilus CRISPR1 (sth1Cas9), Staphylococcus...
Abstract Using an established CRISPR-Cas mediated genome editing technique for streptomycetes, we explored the combinatorial biosynthesis potential of auroramycin biosynthetic gene cluster in Streptomyces roseoporous. Auroramycin is a potent anti-MRSA polyene macrolactam. In addition, it also displays antifungal activities, which unique among structurally similar macrolactams, such as incednine and silvalactam. this work, employed different engineering strategies to target glycosylation...
Abstract The role of water in the ring‐opening rearrangement substituted 1,4,2‐oxathiazoles towards nitriles is examined.
Nature has provided unique molecular scaffolds for applications including therapeutics, agriculture, and food. Due to differences in ecological environments laboratory conditions, engineering is often necessary uncover utilize the chemical diversity. Although we can efficiently activate mine these complex 3D molecules, sufficient production of target molecules further application remain a considerable bottleneck. An example bioactive are armeniaspirols, which potent polyketide antibiotics...