Abstract Two meiotic recombination genes, rec6 and rec12, from Schizosaccharomyces pombe have been cloned by genetic complementation their DNA sequences determined. Gene replacements demonstrated that the fragments contained rec12 genes. Further analysis showed functional gene was within a 1.3-kb fragment 1.7-kb fragment. The nucleotide of these revealed open reading frames (ORFs) predicting 143 amino acids for product 139 product. After pat1-114 temperature-induced meiosis, transcripts were...

Saccharomyces cerevisiae has been intensively studied in responses to different environmental stresses such as heat shock through global omic analysis. However, the S. industrial strains with superior thermotolerance have not explored any proteomic studies for elucidating tolerance mechanism. Recently a new diploid strain was obtained evolutionary engineering of parental strain, and it exhibited even higher resistance prolonged thermal stress. Herein, we performed iTRAQ-based quantitative...

High-temperature fermentation is desirable for the industrial production of ethanol, which requires thermotolerant yeast strains. However, thermotolerance a complicated quantitative trait. The understanding genetic basis behind high-temperature performance still limited. Quantitative trait locus (QTL) mapping by pooled-segregant whole genome sequencing has been proved to be powerful and reliable approach identify loci, genes single nucleotide polymorphism (SNP) variants linked traits yeast....

Genome shuffling was used to improve the acetic acid tolerance of an ethanologenic yeast, Candida krusei GL560. A mutant, S4-3, isolated and selected after four rounds genome shuffling. It found that mutant S4-3 had a higher viability in YNBX (yeast nitrogen base/xylose) medium with grew better YPD extract, peptone dextrose) [1% (w/v) yeast 2% glucose] than parent strain The also improved its multiple stress ethanol, H2O2, heat freeze-thaw. Furthermore, showed ethanol production GL560 EFM...

Saccharomyces cerevisiae cannot utilize cellobiose, but this yeast can be engineered to ferment cellobiose by introducing both cellodextrin transporter (cdt-1) and intracellular β-glucosidase (gh1-1) genes from Neurospora crassa. Here, we report that an S. strain expressing the putative hexose gene HXT2.4 Scheffersomyces stipitis gh1-1 also cellobiose. This result suggests HXT2.4p may function as a when is overexpressed in cerevisiae. However, fermentation was much slower less efficient than...

Droplet-based microfluidics has emerged as a powerful tool for single-cell screening with ultrahigh throughput, but its widespread application remains limited by the accessibility of droplet microfluidic high-throughput (HTS) platform, especially to common laboratories having no background in microfluidics. Here, we first developed HTS platform based on fluorescence-activated sorting technology. This allowed (i) encapsulation single cells monodisperse water-in-oil droplets; (ii) cell growth...

Sugar alcohols have been widely applied in the fields of food and medicine owing to their unique properties. Compared chemical production, microbial production sugar has become attractive because its environmentally friendly sustainable characteristics. Our previous study identified nonconventional yeast Pichia anomala TIB-x229 as a potential producer from glucose. To further improve strain performance, we combined genome shuffling with optimized high throughput screening methods for...

Medium-chain (C8-C14) α, ω-dicarboxylic acids (α, ω-DCAs), which have numerous applications as raw materials for producing various commodities and polymers in chemical industry, are mainly produced from or microbial conversion of petroleum-derived alkanes plant-derived fatty at present. Recently, significant attention has been gained to production medium-chain ω-DCAs simple renewable sugars. Here, we designed created a synthetic omega oxidation pathway Saccharomyces cerevisiae produce C10...

Abstract Background Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals other bioproducts, but challenged by various harsh industrial conditions, such as hyperosmotic, thermal ethanol stresses. Thus, its stress tolerance enhancement has been attracting broad interests. Recently, CRISPR/Cas-based genome editing technology offers unprecedented tools explore genetic modifications performance improvement of S. . Results...

Pichia pastoris is a widely used host organism for heterologous production of industrial proteins, such as cellulases. Although great progress has been achieved in improving protein expression P. pastoris, the potential system not fully explored due to unknown genomic impact factors. Recently, whole-cell directed evolution, employing iterative rounds genome-wide diversity generation and high-throughput screening (HTS), considered be promising strategy strain improvement at genome level.In...

The alcohol dehydrogenase (ADH) system plays a critical role in sugar metabolism involving not only ethanol formation and consumption but also the general "cofactor balance" mechanism. Candida maltosa is able to ferment glucose as well xylose produce significant amount of ethanol. Here we report ADH C. composed three microbial group I genes (CmADH1, CmADH2A CmADH2B), mainly focusing on its metabolic regulation physiological function.Genetic analysis indicated that CmADH2B tandemly located...

To gain a deep understanding of yeast-cell response to heat stress, multiple laboratory strains have been intensively studied via genome-wide expression analysis for the mechanistic dissection classical heat-shock (HSR). However, robust industrial Saccharomyces cerevisiae hardly explored in global elucidation mechanism thermotolerant (TR) during fermentation. Herein, we employed data-independent acquisition and sequential window all theoretical mass spectra based proteomic workflows...

Genome editing is an important tool for building novel genotypes with a desired phenotype. However, the fundamental challenge to rapidly generate alterations on genome-wide scale. Here, we report TALENs (transcription activator-like effector nucleases)-assisted multiplex (TAME), based interaction of designed DNA sequences between critical TATA and GC boxes, generating multiple targeted genomic modifications. Through iterative cycles TAME induce abundant semirational indels coupled efficient...

The fast-growing Vibrio natriegens is an attractive robust chassis for diverse synthetic biology applications. However, V. lacks the suitable constitutive regulatory parts precisely tuning gene expression and, thus, recapitulating physiologically relevant changes in levels. In this study, we designed, constructed, and screened by varying promoter region ribosome binding site element with different transcriptional or translational strengths, respectively. fluorescence intensities of cells...

Abstract Background Prevalence rates of hyperuricemia and gout are increasing. Clinical investigations hyperuricemia-related risk factors aid in the early detection, prevention, management gout. Ongoing research is examining association obesity, dietary patterns, blood pressure (BP) with serum uric acid (sUA). Methods A cross-sectional study was conducted based on National Health Nutrition Examination Survey. The exposures included body mass index (BMI), BP. outcome variable sUA level....

ABSTRACT BACKGROUND To compete with chemical hydrogenation, xylitol production by a biotechnological method has to decrease cost improving the performance of strain, using low‐cost substrate and developing simple efficient bioprocess. This study attempted reach high‐efficient cheap substrate. RESULTS A new evolved Candida maltosa CHH65 showed improved cell growth in corncob hemicellulosic hydrolyzate ( CHH ) similar product xylose as substrate, was developed serial culture transfer for...

ABSTRACT Enhanced capability of co-fermenting glucose and xylose at high temperature is highly desirable for yeast application in second-generation bioethanol production. Here, we obtained hybrid strains with improved glucose-xylose co-fermentation properties by combining genome shuffling adaptive evolution. Genome resequencing these suggested predominantly inherited genetic information from one parental strain Spathaspora passalidarum SP rather than the other Saccharomyces cerevisiae ScY01,...

TALENs-assisted multiplex editing (TAME) toolbox was previously established and used to successfully enhance ethanol stress tolerance of Saccharomyces cerevisiae laboratory strain. Here, the TAME harnessed improve elucidate tolerances S. industrial One osmotolerant strain one thermotolerant were selected from mutant library generated by at corresponding conditions, exhibited 1.2-fold 1.3-fold increases fermentation capacities, respectively. Genome resequencing uncovered genomic alterations...