A5019224205- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Identification and Quantification in Food
- Viral gastroenteritis research and epidemiology
- Heat shock proteins research
- Meat and Animal Product Quality
- Enzyme Catalysis and Immobilization
- Mass Spectrometry Techniques and Applications
- Virus-based gene therapy research
- Protein purification and stability
- Culinary Culture and Tourism
- Archaeology and ancient environmental studies
- RNA Interference and Gene Delivery
- Digestive system and related health
- Microbial Community Ecology and Physiology
- S100 Proteins and Annexins
- Muscle metabolism and nutrition
- Fungal and yeast genetics research
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
National Institutes for Food and Drug Control
2024
CNEX (China)
2022
Beijing Proteome Research Center
2006-2010
University of Florida
2010
Northeast Forestry University
2010
Harbin Normal University
2010
Second Affiliated Hospital of Dalian Medical University
2008
Dalian Medical University
2008
Chinese Academy of Sciences
2008
Dalian Institute of Chemical Physics
2008
Phosphorylation is one of the most important post-translational modifications proteins, which modulates a wide range biological functions and activities proteins. The phosphorylation proteins also associated with pathway cancer cells. We have previously enriched low molecular weight proteome from human plasma based on combination size exclusion adsorption mechanism by using highly ordered mesoporous silica particles. Herein, particles were modified titanium phosphonate to selectively capture...
Drought is one of the most severe limitations to plant growth and productivity. Resurrection plants have evolved a unique capability tolerate desiccation in vegetative tissues. Fern-ally Selaginella tamariscina (Beauv.) primitive vascular resurrection plants, which can survive desiccated state recover when water becomes available. To better understand mechanism tolerance, we applied physiological proteomic analysis. Samples S. were water-deprived for up seven days followed by 12 h...
A new C-type lectin-like gene encodes 293 amino acids and maps to chromosome 19p13.3 adjacent the previously described lectin genes, CD23, dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), DC-SIGN-related protein (DC-SIGNR). The four genes form a tight cluster in an insert size of 105 kb have analogous genomic structures. molecule, designated liver lymph node sinusoidal endothelial cell (LSECtin), is type II integral membrane ∼40 kDa with single domain...
Abstract Recently, a new coronavirus was isolated from the lung tissue of autopsy sample and nasal/throat swabs patients with Severe Acute Respiratory Syndrome (SARS) causative association SARS determined. To reveal further characteristics virus to provide insight about molecular mechanism etiology, proteomic strategy utilized identify structural proteins (SARS‐CoV) Vero E6 cells infected BJ‐01 strain virus. At first, Western blotting convalescent sera demonstrated that there were various...
Background Proteomic approaches based on mass spectrometry have been recently used in archaeological and art researches, generating promising results for protein identification. Little information is known about eastward spread eastern limits of prehistoric milking Eurasia. Methodology/Principal Finding In this paper, an ancient visible food remain from Subeixi Cemeteries (cal. 500 to 300 years BC) the Turpan Basin Xinjiang, China, preliminarily determined containing 0.432 mg/kg cattle...
Stable isotope dilution-multiple reaction monitoring-mass spectrometry (SID-MRM-MS), which is an alternative to immunoassay methods such as ELISA and Western blotting, has been used alleviate the bottlenecks of high-throughput verification biomarker candidates recently. However, inconvenience high consumption required obtain stably labeled peptide impedes broad application this method. In our study, 18O-labeling method was introduced generate stable isotope-labeled peptides instead Fmoc...
Royal jelly (RJ) is a proteinaceous secretion produced from the hypopharyngeal and mandibular glands of nurse bees. It plays vital roles in honeybee biology improvement human health. However, some proteins remain unknown RJ, mapping N-glycosylation modification sites on RJ demands further investigation. We used two different liquid chromatography-tandem mass spectrometry techniques, complementary N-glycopeptide enrichment strategies, bioinformatic approaches to gain better understanding...
A high throughput process including subcellular fractionation and multiple protein separation identification technology allowed us to establish the expression profile of human fetal liver, which was composed at least 2,495 distinct proteins 568 non-isoform groups identified from 64,960 peptides 24,454 peptides. In addition basic mentioned above, MS data were used for complementary novel mining. By doing analysis with integrated protein, expressed sequence tag, genome datasets, 223 15...
Abstract Hepatocellular carcinoma (HCC) is the fifth most common malignant cancer in world. The sensitivity of alpha-fetoprotein (AFP) still inadequate for HCC diagnosis. Tissue interstitial fluid (TIF), as liquid microenvironment cells, was used biomarker discovery this study. Paired tumor and nontumor TIF samples from 6 HBV-HCC patients were analyzed by a proteomic technique named iTRAQ (isobaric tag relative absolute quantitation). Totally, 241 up-regulated proteins (ratio ≥ 1.3, p <...
Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation reactive oxygen species (ROS) homeostasis, physiological diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, transgenic techniques applied validate proteomic results test functions Na2CO3-responsive proteins. A total...
Organelle proteome has become one of the most important fields proteomics, and subcellular fractionation with high purity yield always been a challenge for cell biologists also Human Liver Proteome Project (HLPP). The liver C57BL/6J mouse was chosen as model to find optimum method preparation. we selected could obtain multiple fractions including plasma membrane, mitochondria, nucleus, ER, cytosol from single homogenate. With same procedure, it is first time that preparation frozen...
The unicellular photosynthetic model-organism cyanobacterium Synechocystis sp. PCC6803 can grow photoautotrophically using CO2 or heterotrophically glucose as the sole carbon source. Several pathways are involved in metabolism Synechocystis, and concerted regulation of these by numerous known unknown genes is critical for survival growth organism. Here, we report that a hypothetical protein encoded open reading frame slr0110 necessary heterotrophic Synechocystis. slr0110-deletion mutant...
To gain a deep understanding of yeast-cell response to heat stress, multiple laboratory strains have been intensively studied via genome-wide expression analysis for the mechanistic dissection classical heat-shock (HSR). However, robust industrial Saccharomyces cerevisiae hardly explored in global elucidation mechanism thermotolerant (TR) during fermentation. Herein, we employed data-independent acquisition and sequential window all theoretical mass spectra based proteomic workflows...
During the production of cell and gene therapy products, residual host DNA (HCD) could cause safety risks biological longer HCD fragment, greater risk to human body. For this reason, it was necessary develop an effective method for size distribution analysis fragments with high accuracy sensitivity. In study, capillary gel electrophoresis laser-induced fluorescence detector (CGE-LIF) used analyze in lentivirus products. The results confirmed that lentiviral RNA genome interfere fragments. By...
Abstract Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy, which has three N-glycosylation sites and one O-glycosylation site. It contains variety of different glycosylation modifications, such sialyation, O-acetylation on sialic acids, etc, causes big challenge for analysis rhEPO. In this study, liquid chromatography-mass spectrometry...
Alkali-salinity exerts severe osmotic, ionic and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation reactive oxygen species (ROS) homeostasis, physiological diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, transgenic techniques applied validate proteomic results test functions Na2CO3-responsive proteins. A total...