A5064620582- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Enzyme function and inhibition
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Electrochemical sensors and biosensors
- Biofuel production and bioconversion
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
- interferon and immune responses
- Origins and Evolution of Life
- Galectins and Cancer Biology
- Biochemical and Structural Characterization
- thermodynamics and calorimetric analyses
Institute of Chemistry of the Slovak Academy of Sciences
2003-2024
Slovak Academy of Sciences
2012-2024
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2012-2024
Graz University of Technology
2006-2010
Austrian Centre of Industrial Biotechnology (Austria)
2010
Institute of Molecular Biotechnology
2010
BIOS Bioenergiesysteme (Austria)
2007
Wayne State University
2002-2003
Insoluble protein particles showing high specific enzyme activity are potentially useful biocatalysts. The commercialized crosslinked crystals and aggregates have the disadvantage that their preparation requires isolation of before critical precipitation step. We introduce a novel concept controlled in vivo which target is fused to cellulose-binding domain (CBD) Clostridium cellulovorans, expression Escherichia coli performed under conditions induce selective pull down folded chimeric via...
Active inclusion bodies of polyphosphate kinase 3 and cytidine 5′-monophosphate were combined with whole cells that co-express sialic acid aldolase CMP-sialic synthetase. The biocatalytic mixture was used for the synthesis acid, which then converted to 3′-sialyllactose by cells.
Abstract Background Trigonopsis variabilis D -amino acid oxidase ( Tv DAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands with respect to activity, operational stability and costs also vary field of application. Processes that use soluble suffer from fast inactivation DAO while immobilized preparations raise issues related expensive carriers catalyst efficiency. Therefore, are more robust active than those currently available...
Abstract A one‐step procedure of immobilizing soluble and aggregated preparations D ‐amino acid oxidase from Trigonopsis variabilis ( Tv DAO) is reported where carrier‐free enzyme was entrapped in semipermeable microcapsules produced the polycation poly(methylene‐co‐guanidine) combination with CaCl 2 polyanions alginate cellulose sulfate. The yield immobilization, expressed as fraction original activity present microcapsules, approximately 52 ± 5%. effectiveness for O ‐dependent conversion...
Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing Escherichia coli cells with nonionic detergent and then they immobilized in agar/TiO2 beads. Bioenergy beads are charged to act as rechargeable suppply adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis artificial receptors.
Polyphosphate kinases (PPKs) catalyse the polymerisation and degradation of polyphosphate chains. As a result this process, PPK produces or consumes energy in form ATP. is linear molecule that contains tens to hundreds phosphate residues connected by macroergic bonds, it appears be an easily obtainable rich source from prebiotic times present. Notably, present cells all three domains life, but PPKs are widely distributed only Bacteria, as Archaea Eucarya use various unrelated "nonhomologous"...
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient recycle "gelatinous masses" protein from one reaction cycle another, as high centrifugation forces are needed in large volumes. The magnetization smart solution large-scale applications, enabling easier separation process using magnetic field.Magnetically modified...
Inclusion bodies are typically ignored as they considered unwanted protein waste generated by prokaryotic host cells during recombinant production or harmful inclusions in human cell biology. However, these particles may have applications for vivo immobilization industrial biocatalysis cell-tolerable materials the pharmaceuticals industry and clinical development. Thus, there is a need to “pull-down” (insolubilize) soluble enzymes proteins into inclusion bodies. Accordingly, this study,...
Physiological aggregation of lectin functional domains into active inclusion bodies would provide a simple tool for glycocode reading by well-established agglutination assays.
Abstract Background Adhesins of pathogens recognise the glycans on host cell and mediate adherence. They are also crucial for determining tissue preferences pathogens. Currently, glyco-nanomaterials provide potential tool antimicrobial therapy. We demonstrate that properly glyco-tailored inclusion bodies can specifically bind pathogen adhesins release therapeutic substances. Results In this paper, we describe preparation tailored via conjugation indicator protein aggregated to form with...
[GADV]-protein world hypothesis [1] leaded me to quantification of decapeptides assembled from G, A, V, D in human proteome. The and amino acids were related the nucleotides Guanine (g), Cystosine (c), Uracil (u), Adenine (a). search revealed agreement with genetic code. types prebiotic peptide bonds represent probably first selection power that established base order codons. code underwent three phases formation, which explain why modern codons have their particular nucleotides: monobase,...
Approaches using immobilized biological materials are very promising for application in different branches of the food industry, especially production fermented beverages. Materials tested by our team process entrapment belong to family charged polysaccharides able form beaded hydrogels ionotropic gelation (e.g. alginate, pectate, κ-carrageenan) and synthetic polymers polyvinyl alcohol) forming bead- lens-shaped thermal sol/gel transition. Concentration a gel, conditions instrumentation...
The present study focuses on the application of immobilization technology to enzymic sugar syntheses. paper describes an improved silica-alginate matrix established for entrapment and encapsulation. replacement alginate with pectate provided enhanced chemical resistance matrix, which allows use 1% (w/v) polyphosphate in reaction mixtures. Polylysine, a reagent silica condensation, was replaced by much cheaper alternative, namely polyethyleneimine. proposed design applied production cytidine...