A5040001024- Innovative Energy Harvesting Technologies
- Advanced Sensor and Energy Harvesting Materials
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Amyotrophic Lateral Sclerosis Research
- Tactile and Sensory Interactions
- NF-κB Signaling Pathways
- Wireless Power Transfer Systems
- Conducting polymers and applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenetic and Muscular Disorders Research
- Wind Energy Research and Development
- Pluripotent Stem Cells Research
- Energy Harvesting in Wireless Networks
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Parkinson's Disease Mechanisms and Treatments
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Quinazolinone synthesis and applications
- RNA modifications and cancer
- Cancer-related gene regulation
- Inflammasome and immune disorders
Xidian University
2022-2024
Shaanxi Normal University
2018-2023
Wannan Medical College
2023
Progranulin (PGRN) is a secreted glycoprotein with multiple biological functions in early embryogenesis, anti-inflammation, and neurodegeneration. A good model for the functional study of PGRN zebrafish knockdown or knockout grn, gene encoding PGRN. Morpholino oligonucleotides (MOs) zinc finger nucleases have been used to generate grn models, yet they shown inconsistent phenotypes due either neurotoxicity MOs possible genetic compensation responses during editing. In this study, we generated...
FAM76B has been reported to be a nuclear speckle-localized protein with unknown function. In this study, was first demonstrated inhibit the NF-κB-mediated inflammatory pathway by affecting translocation of hnRNPA2B1 in vitro. We further showed that suppressed inflammation vivo using traumatic brain injury (TBI) mouse model. Lastly, shown interact human tissues taken from patients acute, organizing, and chronic TBI, different neurodegenerative diseases. The results suggested mediated...
Progranulin (PGRN) plays critical roles in inflammation, tumorigenesis, and neurodegeneration. PGRN levels blood cerebrospinal fluid (CSF) are being increasingly investigated as potential biomarkers for these disorders. However, the value of CSF a biomarker has been limited because currently available commercial enzyme-linked immunosorbent assay (ELISA) kits have suboptimal sensitivity detecting PGRN. In this study, pairs monoclonal antibodies (MAbs) were first screened from eleven antiPGRN...
Abstract Mutations in the GRN gene coding for progranulin (PGRN) are responsible many cases of familial frontotemporal lobar degeneration (FTLD) with TAR DNA-binding protein 43 (TDP-43)-positive inclusions (FTLD-TDP). mutations create null alleles resulting decreased or haploinsufficiency. FTLD-TDP is characterized by lentiform neuronal intranuclear that positive TDP-43 affected brain regions. In this study, stably expressed short hairpin RNA, we established a neuroblastoma cell line PGRN...
Abstract Natural wind energy distributes over a wide speed range, but conventional turbines with high electrical damping can only work under relatively speeds, whereas breeze harvesters low suffer from limited electric outputs despite their start-up speeds. To solve this dilemma, we report herein an automatic mode transition (AMT) strategy for rotary (WEHs) to realize self-regulation of according ambient The superior performance achieved the AMT has been demonstrated through AMT-WEH...
Numerous diseases have been connected to protein arginine methylations mediated by methyltransferase 5 (PRMT5). Clinical investigations of the PRMT5-specific inhibitor GSK3326595 are currently being conducted, and results promising for preventing cancers. However, detailed mechanism PRMT5 promoting colorectal cancer (CRC) malignant progression remains unclear. Here, we found that directly catalyzes AlkB homologue (ALKBH5) symmetric dimethylation at R316 residue (meR316-ALKBH5), which...
In order to study the molecular mechanism and physiological significance of interaction between PGRN Rev-erbβ, gene in HEK293 (Rev-erbβ-/-) marked as C3-6 cell lines was knocked out by CRISPR/Cas9 system generate Rev-erbβ double genes knockout lines. First, four sgRNAs were designed for gene, sgRNA2 sgRNA3 with higher activity used construct Lentiviral vector, pLenti/CMV-Loxp-Cas9-sgRNA2-U6-sgRNA3-U6-Loxp-EF1α-Puro. Then, lentivirus vector carrying Cas9 sgRNA infect cells. Through drug...
Abstract FAM76B has been reported to be a nuclear speckle localized protein with unknown function. In this study, was first demonstrated inhibit the NF-κB-mediated inflammatory pathway by affecting translocation of hnRNPA2B1 in vitro. We further showed that suppressed inflammation regulating NF-κB vivo using traumatic brain injury (TBI) model knockout mice. Lastly, shown interact human tissues taken from patients acute, organizing, and chronic TBI, different neurodegenerative diseases. The...