A5048122412- Fungal Biology and Applications
- Plant Reproductive Biology
- Mycorrhizal Fungi and Plant Interactions
- Digital Holography and Microscopy
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Molecular Biology Research
- Advanced Optical Imaging Technologies
- Enzyme-mediated dye degradation
- Plant tissue culture and regeneration
- Advanced Fluorescence Microscopy Techniques
- Fungal Plant Pathogen Control
- Liquid Crystal Research Advancements
- Light effects on plants
- Optical Polarization and Ellipsometry
- Optical Coherence Tomography Applications
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Mycotoxins in Agriculture and Food
- Advanced optical system design
- Plant and fungal interactions
- Optical Systems and Laser Technology
- Pesticide and Herbicide Environmental Studies
- Polysaccharides Composition and Applications
- Microbial Metabolism and Applications
Akita Prefectural University
2011-2024
Food Research Institute
2011
National Agriculture and Food Research Organization
2011
Okayama University
2002-2009
GTx (United States)
2005
Okayama University of Science
1997-2000
The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle 2 weeks, produces some 10 8 synchronized meiocytes, and can be manipulated at all stages by mutation transformation. 37-megabase genome of C. was sequenced assembled into 13 chromosomes. Meiotic recombination rates vary greatly along the chromosomes, retrotransposons are absent large regions with low levels meiotic recombination....
The basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate transcriptome detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. reads were aligned to 14,245...
The homobasidiomycete Coprinus cinereus exhibits remarkable photomorphogenesis during fruiting-body development. Under proper light conditions, primordia proceed to the maturation phase in which basidia pileus undergo meiosis, producing sexual spores, followed by stipe elongation and expansion for efficient dispersal of spores. In continuous darkness, however, do not but are etiolated: tissues at upper part primordium remain rudimentary basal elongates, "dark stipe." this study we...
The formation of the pileus in homobasidiomycete fungi is essential for sexual reproduction, because bears hymenium, a layer cells that includes specialised basidia which nuclear fusion, meiosis and sporulation occur. developmental mutant ichijiku Coprinus cinereus fails to develop differentiated at apex primordial shaft, basal part fruit-body primordia formed an early stage differentiation. Genetic analysis indicates this phenotype caused by recessive mutation single gene (ich1). ich1 was...
ABSTRACT The pileus (cap) of the fruiting body in homobasidiomycete fungi bears hymenium, a layer cells that includes basidia where nuclear fusion, meiosis and sporulation occur. Coprinopsis cinerea is model system for studying development. hymenium C. forms at surface gills pileus. In previous study, we identified mutation called cap-growthless1-1 (cag1-1) blocks gill formation, which yields primordia never mature. this found cag1 gene encodes homologue Saccharomyces cerevisiae Tup1. genome...
It is known that liquid crystal (LC) cells are useful as compact and easy-to-handle phase shifters readily coupled into the optics of standard microscope systems. Here, a uniformly aligned molecular LC shifter introduced polarization to attain birefringence imaging system, using phase-shift interferometric technique. Since can be determined accurately only when optical axis sample parallel or perpendicular slow (variable axis) shifter, an improved data analysis method proposed for...
The developmental biology underlying the morphogenesis of mushrooms remains poorly understood despite essential role fungi in terrestrial environment and global carbon cycle. mushroom Coprinopsis cinerea is a leading model system for molecular cellular basis fungal morphogenesis. dikaryotic vegetative hyphae this fungus grow by tip growth with clamp cell formation, conjugate nuclear division, septation, subapical peg fusion to peg. Studying these processes provides many opportunities gain...
Nematic liquid crystal behaves like an optically uniaxial whose optical axis coincides with the direction of molecular orientation. When electric field is applied, a lateral shear incident light induced, depending on angle inclination. While this may degrade image quality for display applications, precise electrical tunability distance desirable differential interference contrast (DIC) imaging. In Letter, pair twisted nematic (TN) cells used DIC imaging instead normal prisms, and unique...
The C. cinerea septins are observed at the hyphal tip and septum in growing vegetative hyphae. In Saccharomyces cerevisiae, septin dynamics have been suggested to be regulated by phosphorylation through protein kinases such as Cla4. To gain insight into relationship between Cla4 kinase (Cc.Cla4), we conducted disruption of cla4 gene (Cc.cla4) CRISPR/Cas9 system analyzed phenotypes Cc.cla4Δ strains. hyphae strains grow slowly zigzag shape highly branched compared wild-type enhanced green...