A5034777478- BRCA gene mutations in cancer
- Cancer Genomics and Diagnostics
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Acute Myeloid Leukemia Research
- Genomics and Phylogenetic Studies
- Genetic factors in colorectal cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Respiratory viral infections research
- DNA Repair Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- SARS-CoV-2 detection and testing
- Microtubule and mitosis dynamics
- PARP inhibition in cancer therapy
- Viral gastroenteritis research and epidemiology
- Enzyme-mediated dye degradation
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
Hong Kong Sanatorium and Hospital
2016-2025
Rush University Medical Center
2023
University of Hong Kong
2020
Chinese University of Hong Kong
2008-2016
The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle 2 weeks, produces some 10 8 synchronized meiocytes, and can be manipulated at all stages by mutation transformation. 37-megabase genome of C. was sequenced assembled into 13 chromosomes. Meiotic recombination rates vary greatly along the chromosomes, retrotransposons are absent large regions with low levels meiotic recombination....
Background Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of disease are focused on single pathogen Mycobacterium tuberculosis. Recent have revealed associations some diseases with an imbalance microbial community. Characterization TB microbiota could allow better understanding disease. Methodology/Principal Findings Here, sputum infection was examined by using 16S rRNA pyrosequencing. A total 829,873 high-quality sequencing reads were generated from 22...
Genomic techniques in recent years have allowed the identification of many mutated genes important pathogenesis acute myeloid leukemia (AML). Together with cytogenetic aberrations, these gene mutations are powerful prognostic markers AML and can be used to guide patient management, for example selection optimal post-remission therapy. The also hold promise as therapeutic targets themselves. We evaluated applicability a panel detection diagnostic molecular pathology laboratory.Fifty samples...
Abstract Background The transition from the vegetative mycelium to primordium during fruiting body development is most complex and critical developmental event in life cycle of many basidiomycete fungi. Understanding molecular mechanisms underlying this process has long been a goal research on basidiomycetes. Large scale assessment expressed transcriptomes these stages will facilitate generation more comprehensive picture mushroom process. In study, we coupled 5'-Serial Analysis Gene...
An in vitro drug screening using primary AML samples identified homoharringtonine (omacetaxine mepesuccinate) as an effective adjunct for treatment of FLT3-ITD AML.
Abstract Insertions are one of the major types structural variations and defined as addition 50 nucleotides or more into a DNA sequence. Several methods exist to detect insertions from next-generation sequencing short read data, but they generally have low sensitivity. Our contribution is two-fold. First, we introduce INSurVeyor, fast, sensitive precise method that detects paired-end data. Using publicly available benchmark datasets (both human non-human), show INSurVeyor not only than any...
Abstract Leukaemia of various subtypes are driven by distinct chromosomal rearrangement or genetic abnormalities. The leukaemogenic fusion transcripts mutations serve as molecular markers for minimal residual disease (MRD) monitoring. current study evaluated the applicability several droplet digital PCR assays detection these targets at RNA and DNA levels (atypical BCR::ABL1 e19a2, e23a2ins52, e13a2ins74, rare types CBFB::MYH11 (G I), PCM1::JAK2 , KMT2A::ELL2, PICALM::MLLT10 CEBPA...
Abstract Clinical outcome and mutations of 96 core-binding factor acute myeloid leukemia (AML) patients 18–60 years old were examined. Complete remission (CR) after induction was 94.6%. There no significant difference in CR, leukemia-free-survival (LFS) overall survival (OS) between t(8;21) ( N =67) inv(16) =29). Univariate analysis showed hematopoietic stem cell transplantation at CR1 as the only clinical parameter associated with superior LFS. Next-generation sequencing based on a gene...
We reviewed the multiplex PCR results of 20,127 respiratory specimens tested in a hospital setting from January 2014 to April 2023. The seasonal oscillation patterns 17 viruses were studied. Compared with 2014–2019, prominent drop positivity (from 64.46–69.21% 17.29–29.89%, p < 0.001) and virus diversity was observed during COVID-19 pandemic, predominance rhinovirus/enterovirus, sporadic spikes parainfluenza 3 4, syncytial SARS-CoV-2, rare detection influenza viruses, metapneumovirus,...
Laccases are versatile biocatalysts for the bioremediation of various xenobiotics, including dyes and polyaromatic hydrocarbons. However, current sources new enzymes, simple heterologous expression hosts enzymatic information (such as appropriateness common screening substrates on laccase engineering) remain scarce to support efficient engineering better "green" applications. To address issue, this study began with cloning family Lentinula edodes. Three laccases perfectio sensu stricto...
Amplicon-based next-generation sequencing (NGS) has been widely adopted for genetic variation detection in human and other organisms. Conventional data analysis paradigm includes primer trimming before read mapping. Here we introduce BAMClipper that removes sequences after mapping original reads by soft-clipping SAM/BAM alignments. Mutation accuracy was affected the choice of handling approach based on real NGS datasets 7 peripheral blood or breast cancer tissue samples with known...
Detection of chromosomal translocation is a key component in diagnosis and management acute myeloid leukemia (AML). Targeted RNA next-generation sequencing (NGS) emerging as powerful clinically practical tool, but it depends on expression transcript from the underlying DNA translocation. Here, we show clinical utility nanopore long-read rapidly detecting with exact breakpoints. In newly diagnosed patient AML, conventional karyotyping showed t(10;12)(q22;p13) NGS detected NUP98-NSD1 fusion...
Abstract The present study aimed to define a subtype of complex/monosomal karyotype (CK/MK) acute myeloid leukemia (AML) by its distinct clinical features, p53 signaling and responses targeting agents. Ninety‐eight young adults (range: 21‐60 years; median: 49 years) with CK/MK AML were studied. They received standard induction, consolidation allogeneic hematopoietic stem cell transplantation from siblings or matched unrelated donors if available. Chromosomal abnormalities most commonly...
Abstract Background Diversified etiology of lower respiratory tract infection renders diagnosis challenging. The mainstay microbial culture is time-consuming and constrained by variable growth requirements. In this study, we explored the use Nanopore sequencing as a supplementary tool to alleviate diagnostic bottleneck. Methods We developed targeted method based on amplification bacterial 16S rRNA gene fungal internal transcribed spacer region. performance was compared with routine...
The BIOFIRE SPOTFIRE Respiratory (R) Panel is a novel, in vitro diagnostic PCR assay with 15 pathogen targets. runtime about min which the shortest among similar panels market. We evaluated performance of R 151 specimens, including 133 collected from upper respiratory tract (URT), 13 lower (LRT) and 5 external quality assessment program (EQAP) samples. specimens were enrolled throughout first two post-COVID-19 influenza seasons Hong Kong (March to December 2023). For URT full concordance was...
Human fecal carriage of Enterobacteriaceae possessing mobilized colistin resistance genes (mcr-1 and mcr-2) remains obscure in Hong Kong. As part routine surveillance on emerging antibiotic resistance, we conducted a prospective study this topic regional hospital From October 31 to November 25, 2016, all specimens submitted for analysis were included study. These comprised 672 consecutive collected from 616 individuals. Fecal screened colistin-resistant by culture-based method, the presence...
Background Omacetaxine mepesuccinate (OME) has antileukemic effects against acute myeloid leukemia (AML) carrying an internal tandem duplication of Fms‐like tyrosine kinase 3 ( FLT3 ‐ITD). A phase 2 clinical trial was conducted to evaluate a combination treatment sorafenib and omacetaxine (SOME). Methods Relapsed or refractory (R/R) newly diagnosed patients were treated with (200‐400 mg twice daily) OME (2 for 7 (first course) 5 days (second course onward) every 21 until disease progression...