A5100619956- Inflammatory mediators and NSAID effects
- BRCA gene mutations in cancer
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Chromatin Remodeling and Cancer
- Mechanisms of cancer metastasis
- Cancer-related molecular mechanisms research
- Helicobacter pylori-related gastroenterology studies
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Circular RNAs in diseases
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Metastasis and carcinoma case studies
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Synthesis and Biological Evaluation
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Bioactive Compounds and Antitumor Agents
University of Hong Kong
2014-2023
Chinese University of Hong Kong
2011-2022
University of Hong Kong - Shenzhen Hospital
2020-2022
Queen Mary Hospital
2010-2020
City Of Hope National Medical Center
2019
City of Hope
2019
Hong Kong Sanatorium and Hospital
2010-2019
Stanford University
2016
Institut de l'Information Scientifique et Technique
2014
National Yang Ming Chiao Tung University
2004
<h3>Objective:</h3> MicroRNAs (miRNAs) have been shown to offer great potential in the diagnosis of cancer. We investigated whether plasma miRNAs could discriminate between patients with and without colorectal cancer (CRC). <h3>Methods:</h3> This study was divided into three phases: (1) marker discovery using real-time PCR-based miRNA profiling on plasma, corresponding cancerous adjacent non-cancerous colonic tissues five CRC, along from healthy individuals as controls; (2) selection...
We previously showed microRNAs (miRNAs) in plasma are potential biomarkers for colorectal cancer detection. Here, we aimed to develop specific blood-based miRNA assay breast detection.TaqMan-based profiling was performed tumor, adjacent non-tumor, corresponding from patients, and matched healthy controls. All putative markers identified were verified a training set of patients. Selected validated case-control cohort 170 100 controls, 95 other types cancers then blindly an independent 70...
Abstract Triple-negative breast cancer (TNBC) is a malignant subtype of with the absence targeted therapy, resulting in poor prognosis patients. Chemotherapy remains mainstay treatment for TNBC; however, development drug resistance main obstacle successful treatments. In recent years, long non-coding RNA (lncRNA) has been implicated multiple biological functions various diseases, particularly cancers. Accumulating evidence suggested that lncRNA nuclear paraspeckle assembly transcript 1...
Abstract Chemoresistance remains the uppermost disincentive for cancer treatment on account of many genetic and epigenetic alterations. Long non-coding RNAs (lncRNAs) are emerging players in promoting initiation progression. However, regulation function chemoresistance largely unknown. Herein, we identified ARHGAP5-AS1 as a lncRNA upregulated chemoresistant gastric cells its knockdown reversed chemoresistance. Meanwhile, high expression was associated with poor prognosis patients....
Cigarette smoking is a risk factor for colorectal cancer. It suggested that 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), tobacco-specific nitrosamine, mediates the carcinogenic action of cigarette by promoting cancer growth. In present study, proliferative response cultured colon cell line HT-29 to NNK was determined. found dose-dependently stimulated proliferation. this regard, stimulatory abolished atenolol and ICI 118,551, beta1- beta2-selective antagonist, respectively....
Cigarette smoke is one of the risk factors for gastric cancer and nicotine has been reported to promote tumor growth. Deregulation microRNA (miRNA) cyclooxygenase-2 (COX-2) expressions are hallmarks many cancers including cancer. Here, we used an miRNA array platform covering a panel 95 human miRNAs examine expression profile in nicotine-treated cells. We found that miR-16 miR-21 were upregulated upon stimulation, transfection with anti-miR-16 or anti-miR-21 significantly abrogated cell...
Triple-negative breast cancer (TNBC) accounts for 15–20% of all in women globally. This subtype often has early and high recurrence rates resulting poor survival, partially due to lack targeted therapies. Therefore, there is an urgent need identify TNBC-specific biomarkers diagnosis treatment monitoring, develop more effective therapy. By using miRCURY LNA array platform, we compared the differential miRNA expressions plasma patient with TNBC (n=5) non-TNBC (n=5), as well healthy controls...
Triple-negative breast cancer (TNBC) remains a poor prognostic factor for since no effective targeted therapy is readily available. Our previous studies confirmed miR-199a-5p TNBC-specific circulating biomarker, however, its functional roles in largely unknown. Thus, we investigated the implication of TNBC and potential underlying mechanisms. MTT assay was performed to investigate cell proliferation after transient transfection MDA-MB-231 line, followed by cycle analysis. Transwell invasion...
Abstract Chromatin remodeling has emerged as a hallmark of gastric cancer, but the regulation chromatin regulators other than genetic change is unknown. Helicobacter pylori causes epigenetic dysregulation to promote carcinogenesis, roles and functions microRNAs (miRNA) in this multistage cascade are not fully explored. In study, miRNA expression preneoplastic neoplastic lesions murine stomachs induced by H. N-methyl-N-nitrosourea (MNU) was profiled array. miR-490-3p exhibited progressive...
Despite the declining incidence of gastric cancer, mortality rate remains high due to late presentation. We aimed evaluate sensitivity miRNA as a diagnostic marker for cancer in circulation.Plasma samples from 3 independent groups comprise 123 patients and 111 healthy controls profiling microarray screening.Microarray data showed that 25 miRNAs were upregulated 6 highly expressed (miR-18a, miR-140-5p, miR-199a-3p, miR-627, miR-629 miR-652) selected validation. In an validation set, levels...
Early studies revealed that cigarette smoke promotes gastric cancer growth through the induction of cyclooxygenase-2 (COX-2). Nicotine, one active ingredients in smoke, has detrimental effects stomach. To date, there is no direct evidence to validate effect nicotine on tumor and its carcinogenic mechanism(s). We therefore investigated whether could promote neovascularization vivo, biological mechanism(s) connection with signaling cascade involving COX-2 extracellular signal-regulated protein...
MicroRNAs (miRNAs) are small non-protein-coding RNAs that regulate expression of a wide variety genes including those involved in cancer development. Here, we investigate the role miR-143 breast cancer. In this study, showed was frequently downregulated 80% carcinoma tissues compared to their adjacent noncancerous tissues. Ectopic inhibited proliferation and soft agar colony formation cells also DNA methyltransferase 3A (DNMT3A) on both mRNA protein levels. Restoration reduces PTEN...
Abstract Blockade of angiogenesis is a promising strategy to suppress tumor growth, invasion, and metastasis. Vascular endothelial growth factor (VEGF), which binds tyrosine kinase receptors [VEGF (VEGFR) 1 2], the mediator mitogen for cells. Cyclooxygenase-2 (COX-2) plays an important role in promoting action nicotine on gastric cancer growth. However, relationship between COX-2 VEGF/VEGFR system tumorigenesis remain undefined. In this study, effects angiogenesis, invasiveness, metastasis...
Chemoresistance is one of the most important challenges in clinical management lung cancer. SIRT1 a NAD dependent protein deacetylase and implicated diverse cellular processes such as DNA damage repair, cancer progression. upregulated chemoresistant cells, genetic knockdown or chemical inhibition reversed chemoresistance by enhancing apoptosis activation, accompanied with XRCC1 degradation. E3 ligase β-TrCP catalyzed poly-ubiquitination to promote its proteasome-dependent bound deacetylated...
Nicotine [3-(1-methyl-2-pyrrolidinyl)-pyridine], a major alkaloid in tobacco, has been implicated as playing role carcinogenesis. Our previous study showed that passive cigarette smoking promoted inflammation-associated colonic adenoma formation mice, and 5-lipoxygenase (5-LOX) plays an important this process. In the present study, we aimed to investigate whether nicotine could stimulate colon cancer cell proliferation tumor growth nude mice xenograft model possible mechanisms involved....