A5076943977- RNA modifications and cancer
- Lipoproteins and Cardiovascular Health
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Viral Infectious Diseases and Gene Expression in Insects
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Chemical and Physical Properties in Aqueous Solutions
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Thermodynamic properties of mixtures
- Circular RNAs in diseases
- Cancer-related gene regulation
- Nutritional Studies and Diet
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Microbial Metabolism and Applications
- Silk-based biomaterials and applications
- Cardiac Fibrosis and Remodeling
- CRISPR and Genetic Engineering
- Chemical Thermodynamics and Molecular Structure
- Sirtuins and Resveratrol in Medicine
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2024
Central South University
2019-2024
Sir Run Run Shaw Hospital
2016-2024
Zhejiang University
2016-2024
Ningbo University
2024
Shanghai Power Equipment Research Institute
2024
Second Affiliated Hospital of Zhejiang University
2024
Second Xiangya Hospital of Central South University
2019-2023
University of Science and Technology of China
2023
Hunan Provincial Center for Disease Control and Prevention
2020-2023
Protein acetylation emerged as a key regulatory mechanism for many cellular processes. We used genetic analysis of Saccharomyces cerevisiae to identify Esa1 histone acetyltransferase required autophagy. further identified the autophagy signaling component Atg3 substrate Esa1. Specifically, K19 and K48 regulated by controlling Atg8 interaction lipidation Atg8. Starvation induced transient K19-K48 through spatial temporal regulation localization acetylase deacetylase Rpd3 on pre-autophagosomal...
Abstract Chemoresistance remains the uppermost disincentive for cancer treatment on account of many genetic and epigenetic alterations. Long non-coding RNAs (lncRNAs) are emerging players in promoting initiation progression. However, regulation function chemoresistance largely unknown. Herein, we identified ARHGAP5-AS1 as a lncRNA upregulated chemoresistant gastric cells its knockdown reversed chemoresistance. Meanwhile, high expression was associated with poor prognosis patients....
Cuproptosis and ferroptosis are the two newly defined metal-related regulated cell death. However, crosstalk between cuproptosis is obscure.We analyzed effect of inducers on copper ionophores-induced death through CCK-8 assay. was studied using immunofluorescence protein soluble-insoluble fraction isolation. GSH assay, qRT-PCR western blot were adopted to explore machinery enhanced cuproptosis. And mouse xenograft model built detect synergy elesclomol-Cu sorafenib in vivo.Herein we found...
Abstract Titanium dioxide nanoparticles (TiO 2 NPs) are widely used in toothpastes, sunscreens, and products for cosmetic purpose that the human use daily. Although neurotoxicity induced by TiO NPs has been demonstrated, very little is known about molecular mechanisms underlying brain cognition behavioral injury. In this study, mice were exposed to 2.5, 5, 10 mg/kg body weight (BW) nasal administration 90 consecutive days, respectively, their brains' injuries gene‐expressed profile...
Abstract A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few confer light-switchable a generic target interest. Such capability would offer substantial advantages, enabling photoswitchable endogenous proteins in cells or light-based purification vitro. Here, we report the development opto-nanobodies (OptoNBs), versatile class chimeric whose interest can be enhanced inhibited upon blue...
Gliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor is overexpressed in gliomas, can promote gliomagenesis by modulating expression oncogenic microRNA-23a (mir-23a). First, found CREB highly expressed glioma tissues cell lines. also essential for growth survival vitro critical vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, luciferase...
Abstract Background Heat shock transcription factor1 (HSF1) was overexpressed to promote glutaminolysis and activate mTOR in colorectal cancer (CRC). Here, we investigated the mechanism for cancer-specific overexpression of HSF1. Methods HSF1 expression analyzed by chromatin immunoprecipitation, qRT-PCR, immunohistochemistry staining immunoblotting. translation explored polysome profiling nascent protein analysis. Biotin pulldown m6A RNA immunoprecipitation were applied investigate RNA/RNA...
As glutamine plays a central role in cancer metabolism, inhibition of glutaminolysis has become an ideal anticancer therapeutic target.However, leads to activation autophagy, which compromises its antitumor effect.Hence, we investigated the mechanism underlying inhibition-induced pro-survival autophagy.Methods: High-throughput sequencing was performed on colorectal (CRC) cells before and after identify differentially expressed genes.Activating transcription factor 4 (ATF4) pathway enrichment...
Background: Timely and complete restoration of blood flow is the most effective intervention for patients with acute myocardial infarction. However, efficacy limited by ischemia-reperfusion (MI/R) injury. PDE4 (phosphodiesterase-4) hydrolyzes intracellular cyclic adenosine monophosphate it has 4 subtypes A-D. This study aimed to delineate role PDE4B (phosphodiesterase-4 subtype B) in MI/R Methods: Mice were subjected 30-minute coronary artery ligation, followed 24-hour reperfusion. Cardiac...
Migration-proliferation dichotomy is a common mechanism in gliomagenesis; however, an understanding of the exact molecular this "go or grow" phenomenon remains largely incomplete. In present study, we first found that microRNA-9 (miR-9) highly expressed glioma cells. MiR-9 inhibited proliferation and promoted migration cells by directly targeting cyclic AMP response element-binding protein (CREB) neurofibromin 1 (NF1), respectively. Our data also suggested migration-inhibitory role for CREB...
Background: Genome-wide association studies identified the of CXCL12 genetic locus (which encodes chemokine CXCL12, also known as stromal cell–derived factor 1) with coronary artery disease and myocardial infarction (MI). Unlike CXCR4, classic receptor for function CXCR7 (the most recently receptor) in vascular responses to injury MI remains unclear. Methods: Tissue expression was examined arteries from mice humans. Mice that harbored floxed Cdh5 -promoter driven CreERT2 were treated...
The gut microbiota has a crucial role in the growth, development and environmental adaptation host insect. objective of our work was to investigate healthy silkworm Bombyx mori changes after infection B. cypovirus (BmCPV). Intestinal contents infected larvae fifth instar were collected at 24, 72 144 h post with BmCPV. bacteria analyzed by pyrosequencing 16S rRNA gene. 147(135) 113(103) genera found content control female (male) BmCPV-infected larvae, respectively. In general, microbial...
Abstract The use of nonsteroidal anti-inflammatory drugs that inhibit cyclooxygenase (COX)-1 and COX-2, increases heart failure risk. It is unknown whether microsomal (m) prostaglandin (PG) E synthase (S)-1, a target downstream COX, regulates myocardial (M) ischemia/reperfusion (I/R) injury, key determinant failure. Here we report COX-1 mPGES-1 mediate production substantial amounts PGE 2 confer cardiac protection in MI/R. Deletion mPges-1 impairs microvascular perfusion inflammatory cell...