A5050213589- MicroRNA in disease regulation
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Genetics and Neurodevelopmental Disorders
- Virus-based gene therapy research
- Nerve injury and regeneration
- Viral Infections and Immunology Research
- Neurogenesis and neuroplasticity mechanisms
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- interferon and immune responses
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Wnt/β-catenin signaling in development and cancer
- Chronic Myeloid Leukemia Treatments
- RNA regulation and disease
- Galectins and Cancer Biology
Qingdao University
2021-2025
Affiliated Hospital of Qingdao University
2021-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Jiangnan University
2024-2025
Soochow University
2010-2024
First Affiliated Hospital of Soochow University
2010-2024
Nanjing Medical University
2018-2024
Wuxi No.2 People's Hospital
2018-2024
Xiamen University
2023
Fujian Normal University
2023
Abstract Neurological manifestations are frequently reported in the COVID-19 patients. Neuromechanism of SARS-CoV-2 remains to be elucidated. In this study, we explored mechanisms neurotropism via our established non-human primate model COVID-19. rhesus monkey, invades CNS primarily olfactory bulb. Thereafter, viruses rapidly spread functional areas central nervous system, such as hippocampus, thalamus, and medulla oblongata. The infection induces inflammation possibly by targeting neurons,...
Gliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor is overexpressed in gliomas, can promote gliomagenesis by modulating expression oncogenic microRNA-23a (mir-23a). First, found CREB highly expressed glioma tissues cell lines. also essential for growth survival vitro critical vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, luciferase...
// Jia Ma 1, 2 , Binbin Fang 3 Fanpeng Zeng Cong Haijie Pang Long Cheng 4 Ying Shi Hui Wang Bin Yin 1 Jun Xia Zhiwei The Cyrus Tang Hematology Center and Collaborative Innovation of Hematology, Jiangsu Institute First Affiliated Hospital, Soochow University, Suzhou 215123, China Department Biochemistry Molecular Biology, Bengbu Medical College, Anhui 233030, Research Clinical Laboratory Science, Laboratory, Yijishan Wannan Wuhu 241000, Anhui, Correspondence to: Wang, e-mail:...
PCBP2 is a member of the poly(C)-binding protein (PCBP) family, which plays an important role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs target mRNAs. Several PCBP family members have been reported to be involved human malignancies. Here, we show that upregulated glioma tissues cell lines. Knockdown inhibited growth vitro vivo through inhibition cell-cycle progression induction caspase-3-mediated apoptosis. Thirty-five mRNAs were...
Migration-proliferation dichotomy is a common mechanism in gliomagenesis; however, an understanding of the exact molecular this "go or grow" phenomenon remains largely incomplete. In present study, we first found that microRNA-9 (miR-9) highly expressed glioma cells. MiR-9 inhibited proliferation and promoted migration cells by directly targeting cyclic AMP response element-binding protein (CREB) neurofibromin 1 (NF1), respectively. Our data also suggested migration-inhibitory role for CREB...
Although the roles of circadian Clock genes and microRNAs in tumorigenesis have been profoundly studied, mechanisms cross‐talk between them regulation gliomagenesis are poorly understood. Here we show that expression level CLOCK is significantly increased high‐grade human glioma tissues glioblastoma cell lines. In contrast miR‐124 attenuated similar samples. Further studies a direct target miR‐124, either restoration or silencing can reduce activation NF‐κB. conclusion, suggest as suppressor...
Acidosis is a significant feature of the tumor microenvironment in glioma, and it closely related to multiple biological functions cancer stem cells. Here, we found that self-renewal ability, mitochondrial activity ATP production were elevated cell-like glioma cells (SLCs) under acidic microenvironment, which promoted maintained stemness SLCs. Under acidosis, 25-hydroxy vitamin D3-24-hydroxylase (CYP24A1) was upregulated catalyzed fast degradation 1α,25(OH)2D3. We further revealed active...
Aim: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world. There is urgent need to understand phylogeny, divergence and origin of SARS-CoV-2. Materials & methods: A recent study claimed that there was 17% between SARS-CoV-2 RaTG13 (a SARS-related coronaviruses) on synonymous sites by using sequence alignment. We re-analyzed sequences two coronaviruses with same methodology. Results: found 87% substitutions could be potentially explained RNA...
Background Asthma is a chronic inflammatory condition of the airways, leading to excessive production mucus and an lungs. The primary clinical manifestations asthma comprise thoracic constriction, coughing, wheezing, dyspnea. Purpose present work was designed examine therapeutic effects aucubin in treating ovalbumin (OVA)-induced mice via suppressing responses. Methods aucubin-induced RAW 264.7 cell viability assessed using MTT test. cytokine levels lipopolysaccharide (LPS)-treated cells...
Abstract The development of the mammalian neocortex is precisely regulated by temporal gene expression, yet regulatory mechanisms cortical neurogenesis, particularly how radial glial cells (RGCs) sequentially generate deep to superficial neurons, remain unclear. Here, hnRNP family member Syncrip (hnRNP Q) identified as a key modulator neuronal differentiation in neocortical neurogenesis. knockout RGCs disrupts and abnormal localization, ultimately resulting layer defects well learning memory...
Polypyrimidine tract-binding protein 1 (PTBP1) involving in almost all steps of mRNA regulation including alternative splicing metabolism during tumorigenesis due to its RNA-binding activity. Initially, we found that high expressed PTBP1 and poor prognosis was interrelated colorectal cancer (CRC) patients with stages II III CRC, which widely different treatment, by immunohistochemistry. also upregulated colon cell lines. In our study, knockdown siRNA transfection decreased proliferation...
Abstract In our study, we aimed to investigate the role of CDR1as during competitive inhibition miR‐7 in regulation cisplatin chemosensitivity breast cancer via regulating REGγ. RT‐qPCR was applied detect expression and tissues, cell lines corresponding drug‐resistant lines. The correlation between REGγ evaluated. MCF‐7‐R MDA‐MB‐231‐R cells were selected followed by transfection a series mimics, inhibitors or siRNA. effect on half maximal inhibitor concentration (IC50), sensitivity apoptosis...
Gliomas account for approximately 80% of primary malignant tumors in the central nervous system. Despite aggressive therapy, prognosis patients remains extremely poor. Glioma stem cells (GSCs) which considered as potential target therapy their crucial role therapeutic resistance and tumor recurrence, are believed to be key factors disappointing outcome. Here, we took advantage GSCs cell model perform high-throughput drug screening old antibiotic, clofoctol, was identified most effective...
Understanding how SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) efficiently reproduces itself by taking resources from the human host could facilitate development of drugs against virus. translates its own proteins using tRNAs, so that GC or codon usage should fit cells. It is necessary to study both virus and genomes in light evolution adaptation. The has significantly lower content GC3 as compared human. However, when we selected a set genes have similar properties...
Background: Acute myeloid leukemia (AML) is an aggressive cancer with variable treatment responses. While clinical factors such as age and genetic mutations contribute to prognosis, recent studies suggest that CT attenuation scores may also predict outcomes. This study aims develop a nomogram combining CT-based response guide personalized therapy for AML patients. Methods: retrospective included 74 newly diagnosed patients who underwent unenhanced abdominal scans within one week before...
Abstract Background microRNAs (miRNAs) are shown to be involved in the regulation of circadian clock. However, it remains largely unknown whether miRNAs can regulate core clock genes ( Clock and Bmal1 ). Results In this study, we found that mir-142-3p directly targeted 3’UTR human BMAL1 mouse . The over-expression (in 293ET NIH3T3 cells) knockdown U87MG reduced up-regulated Bmal1/BMAL1 mRNA protein levels, respectively. Moreover, expression level oscillated serum-shocked cells results ChIP...
Abstract The accurate generation of an appropriate number different neuronal and glial subtypes is fundamental to normal brain functions requires tightly orchestrated spatial temporal developmental programmes maintain the balance between proliferation differentiation neural progenitor cells. However, molecular mechanism governing this process has not been fully elucidated. Here, we found that miR-214-3p was highly expressed in cells dynamically regulated during neocortical development....
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in sumoylation pathway, is up-regulated many cancers. However, expression and regulation of UBC9 glioma remains unknown. In this study, we found Ubc9 was tissues cell lines compared to a normal control. knockdown by small interfering RNA (siRNA) affected proliferation apoptosis T98G cells. Further experiments revealed microRNA (miR)-214 directly targeted 3' untranslated region (UTR) there an inverse...