A5040114152- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- 14-3-3 protein interactions
- Epigenetics and DNA Methylation
- Plant Gene Expression Analysis
- Acute Lymphoblastic Leukemia research
- Plant Stress Responses and Tolerance
- Bacterial Genetics and Biotechnology
- Vector-Borne Animal Diseases
- Cassava research and cyanide
- Chronic Myeloid Leukemia Treatments
- RNA regulation and disease
- Expert finding and Q&A systems
- Drug Transport and Resistance Mechanisms
- Natural Language Processing Techniques
- Toxoplasma gondii Research Studies
- Calcium signaling and nucleotide metabolism
Heilongjiang Bayi Agricultural University
2024-2025
Southwestern Medical Center
2017-2024
The University of Texas Southwestern Medical Center
1992-2024
Howard Hughes Medical Institute
2023-2024
Harvard University
2023-2024
Xi'an Medical University
2022
Shandong Normal University
2019
Yale University
2019
China Agricultural University
2013-2018
Tsinghua University
2012-2015
Protein acetylation emerged as a key regulatory mechanism for many cellular processes. We used genetic analysis of Saccharomyces cerevisiae to identify Esa1 histone acetyltransferase required autophagy. further identified the autophagy signaling component Atg3 substrate Esa1. Specifically, K19 and K48 regulated by controlling Atg8 interaction lipidation Atg8. Starvation induced transient K19-K48 through spatial temporal regulation localization acetylase deacetylase Rpd3 on pre-autophagosomal...
Polycomb repressive complexes 1 and 2 (PRC1 2) are required for heritable repression of developmental genes. The cis- trans-acting factors that contribute to epigenetic inheritance mammalian not fully understood. Here, we show that, in human cells, ectopically induced silencing at initially active genes, but near ubiquitously expressed housekeeping is inherited many cell divisions. Unexpectedly, cells with mutations the H3K27me3 binding pocket Embryonic Ectoderm Development (EED) subunit...
Autophagy is a conserved eukaryotic lysosomal degradation pathway that responds to environmental and cellular cues. essential for the meiotic exit sporulation in budding yeast, but underlying molecular mechanisms remain unknown. Here, we show autophagy maintained during meiosis stimulated anaphase I II. Cells with higher levels of complete faster, genetically enhanced increases kinetics efficiency. Strikingly, our data reveal phosphatase Cdc14 regulates meiosis-specific autophagy. activated...
The rixosome and PRC1 silencing complexes are associated with deSUMOylating deubiquitinating enzymes, SENP3 USP7, respectively. How deSUMOylation deubiquitylation contribute to rixosome- Polycomb-mediated is not fully understood. Here, we show that the enzymatic activities of USP7 required for Polycomb target genes. deSUMOylates several subunits, this activity association PRC1. associates canonical (cPRC1) deubiquitinates chromodomain subunits CBX2 CBX4, inhibition USP results in disassembly...
In yeast meiosis, autophagy is active and essential. Here, we investigate the fate of Rim4, a meiosis-specific RNA-binding protein (RBP), its associated transcripts during meiotic autophagy. We demonstrate that Rim4 employs nuclear localization signal (NLS) to enter nucleus, where it loads mRNA substrates before export. Upon reaching cytoplasm, selectively spares Rim4-mRNA complex. During divisions, preferentially degrades in an Atg11-dependent manner, coinciding with release Rim4-bound...
Autophagy is an evolutionarily conserved cellular process which degrades intracellular contents. The Atg17-Atg31-Atg29 complex plays a key role in autophagy induction by various stimuli. In yeast, occurs with autophagosome formation at special site near the vacuole named pre-autophagosomal structure (PAS). forms scaffold for PAS organization, and recruits other autophagy-related (Atg) proteins to PAS. Here, we show that Atg31 phosphorylated protein. phosphorylation sites on were identified...
Phage display technology has become an important research tool in biological research, fundamentally changing the traditional monoclonal antibody preparation process, and been widely used establishment of antigen-antibody libraries, drug design, vaccine pathogen detection, gene therapy, antigenic epitope cellular signal transduction research.The phage is a powerful platform for development. Using technology, single chain fragment variable (scFv) can be screened, replacing disadvantage large...
Maize is an important food crop and its fungal disease has become a limiting factor to improve the yield quality of maize. In control plant pathogens, commercial fungicides have no obvious effect on corn diseases due emergence drug resistance. Therefore, it great significance develop new with novel structure, high efficiency, low toxicity maize diseases. this paper, series 1,3,4-oxadiazole derivatives were designed synthesized from benzoyl hydrazine aromatic aldehydes through condensation...
Rim4 is a meiosis-specific RNA-binding protein (RBP) that sequesters mRNAs to suppress their translation. Previous work has defined the C-terminal low-complexity domain (LCD) as sequences form self-propagating amyloid-like aggregates. Here, we uncovered dynamic and reversible of self-assembly primarily triggered by heat during meiosis, proportionally from 30°C 42°C. The formed thermal condensates in cell promptly stimulates stress granule (SG) assembly, recruiting SG-resident proteins, such...
<title>Abstract</title> The mdtE and mdtF genes encode membrane transporters, with their transport substrates mechanisms still not fully understood. Amino acid sequence analysis suggests that MdtE belongs to the MFP family while MdtF RND family. is positioned in inner membrane, found periplasmic space, connecting outer porin TolC create a MdtEF-TolC tripartite efflux pump for drug substrate efflux. Subsequently, an Escherichia coli (E. coli) mdtEF gene deletion strain was developed using...
Background Bacteria can develop resistance to various antibiotics under selective pressure, leading multifaceted changes in mechanisms. Transcriptomic sequencing allows for the observation of transcriptional level alterations cells antibiotic stress. Understanding bacterial response such stress is essential deciphering their strategy against drug-resistant and identifying potential targets development. Methods This study using wild-type (WT) Escherichia coli ( E. ) discovered that continuous...
6083 Background: While compliance to drug therapy is vital receive optimal patient benefits, the costs of delivering adequate medical care for cancer patients remain an important consideration society and payers. This study examined relationship between with IM health CML GIST. Methods: Claims data from 6/1/01–3/31/04 a US plan were used identify non-Medicare IM-treated or GIST diagnosis who had continuous pharmacy benefits in 3 months prior 12 following initiation therapy. Compliance was...
Abstract In budding yeast, Rim4 sequesters a subset of meiotic transcripts and essentially suppresses their translation until being degraded at the end meiosis I. We found that loads mRNAs in nucleus as prerequisite for relocating into cytoplasm, where protect from autophagy. Nonetheless, underlying mechanism remains unknown. Using genetic, biochemical, cell imaging approaches, here, we revealed phosphorylation states regulate Rim4’s intracellular interactions with yeast 14-3-3 proteins Bmh1...
Abstract Autophagy can degrade RNA, with an intricate preference for specific RNAs. However, the mechanism remains unclear. During yeast meiosis, autophagy is active, while a subset of transcripts needs to survive until their programmed translation during and at end meiotic divisions. Thus, challenge here how temporally spares mRNAs. Rim4, meiosis-specific RNA binding protein (RBP), sequesters set mid-late early meiosis suppress premature translation. Recently, we reported that degrades...
In Saccharomyces cerevisiae, macroautophagy/autophagy plays a pivotal role and is indispensable for multiple meiotic processes. this study, we demonstrate that Rim4, meiosis-specific RNA-binding protein (RBP) holds back the translation of specific subset transcripts until its programmed degradation by autophagy during divisions, forms heterotrimeric complex in vivo with yeast YWHA/14-3-3 proteins Bmh1 Bmh2, which effectively expels mRNAs from Rim4's binding grip. We pinpoint four distinct...