A5046860131- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
- Plant tissue culture and regeneration
- Food Industry and Aquatic Biology
- Folate and B Vitamins Research
- Advanced Scientific Research Methods
- Plant Reproductive Biology
- Metabolism and Genetic Disorders
- Seed Germination and Physiology
- Hepatitis C virus research
- Methemoglobinemia and Tumor Lysis Syndrome
- Plant responses to water stress
- Genomics, phytochemicals, and oxidative stress
- ATP Synthase and ATPases Research
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- Plant Genetic and Mutation Studies
- Metabolism, Diabetes, and Cancer
University Hospital St. Ivan Rilski
2011-2024
ARC Centre of Excellence in Plant Energy Biology
2009-2023
The University of Western Australia
2012-2023
Agrobioinstitute
1994-2022
Agricultural Academy
2022
Siberian Federal University
2009-2021
Université Paris Cité
2019
Australian Research Council
2008-2019
La Trobe University
2014-2017
Commonwealth Scientific and Industrial Research Organisation
2015
Up-regulation of hepatic delta-aminolevulinic acid synthase 1 (ALAS1), with resultant accumulation (ALA) and porphobilinogen, is central to the pathogenesis acute attacks chronic symptoms in porphyria. Givosiran, an RNA interference therapy, inhibits ALAS1 expression.In this double-blind, placebo-controlled, phase 3 trial, we randomly assigned symptomatic patients porphyria receive either subcutaneous givosiran (2.5 mg per kilogram body weight) or placebo monthly for 6 months. The primary...
Treatment of Arabidopsis (Arabidopsis thaliana) alternative oxidase1a (aox1a) mutant plants with moderate light under drought conditions resulted in a phenotypic difference compared ecotype Columbia (Col-0), as evidenced by 10-fold increase the accumulation anthocyanins leaves, alterations photosynthetic efficiency, and increased superoxide radical reduced root growth at early stages seedling growth. Analysis metabolite profiles revealed significant changes upon treatment aox1a typical...
Upon disturbance of their function by stress, mitochondria can signal to the nucleus steer expression responsive genes. This mitochondria-to-nucleus communication is often referred as mitochondrial retrograde regulation (MRR). Although reactive oxygen species and calcium are likely candidate signaling molecules for MRR, protein components in plants remain largely unknown. Through meta-analysis transcriptome data, we detected a set genes that common robust targets MRR used them bait identify...
Abstract Plants require daily coordinated regulation of energy metabolism for optimal growth and survival therefore need to integrate cellular responses with both mitochondrial plastid retrograde signaling. Using a forward genetic screen characterize regulators alternative oxidase1a (rao) mutants, we identified RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as direct positive regulator AOX1a. RAO2/ANAC017 is targeted connections junctions in the endoplasmic reticulum (ER) F-actin...
Background and Aims Acute hepatic porphyria comprises a group of rare genetic diseases caused by mutations in genes involved heme biosynthesis. Patients can experience acute neurovisceral attacks, debilitating chronic symptoms, long‐term complications. There is lack multinational, prospective data characterizing the disease current treatment practices severely affected patients. Approach Results EXPLORE prospective, natural history study activity clinical management patients with who...
Abstract Transcriptome and metabolite profiling of rice (Oryza sativa) embryo tissue during a detailed time course formed foundation for examining transcriptional posttranscriptional processes germination. One hour after imbibition (HAI), independent changes in transcript levels, rapid metabolism occurred, including increases hexose phosphates, tricarboxylic acid cycle intermediates, γ-aminobutyric acid. Later the metabolome, those involved carbohydrate, amino acid, cell wall metabolism,...
Abstract Background Reference genes are widely used to normalise transcript abundance data determined by quantitative RT-PCR and microarrays. However, the approaches taken define reference can be variable. Although Oryza sativa (rice) is a model plant important crop specie, there has been no comprehensive analysis carried out superior genes. Results Analysis of 136 Affymetrix transcriptome datasets comprising 373 genome microarrays from studies in rice that encompass tissue, developmental,...
Abstract Analysis reveals that there is limited overlap in the sets of transcripts show significant changes abundance during anaerobiosis different plant species. This may be due to fact a combination primary effects, presence or absence oxygen, and secondary responses tissue developmental responses, are measured together not differentiated from each other. In order dissect out these effect oxygen was investigated using three experimental designs rice (Oryza sativa) as model system. A total...
Abstract The perception and integration of stress stimuli with that mitochondrion function are important during periods perturbed cellular homeostasis. In a continuous effort to delineate these mitochondrial/stress-interacting networks, forward genetic screens using the mitochondrial response marker alternative oxidase 1a (AOX1a) provide useful molecular tool identify characterize regulators signaling (referred as [RAOs] components). this study, we reveal mutations in genes coding for...
Previous studies have identified a range of transcription factors that modulate retrograde regulation mitochondrial and chloroplast functions in Arabidopsis (Arabidopsis thaliana). However, the relative importance these regulators whether they act downstream separate or overlapping signaling cascades is still unclear. Here, we demonstrate multiple stress-related pathways, with distinct kinetic signatures, converge on gene sets involved energy organelle function. The factor ANAC017 almost...
The grape and wine industries are heavily reliant on sulphite preservatives. However, the view that sulphites act directly bacterial fungal pathogens may be simplistic. Mechanisms of sulphur-enhanced defences largely unknown; many sulphur-rich compounds enhance plant can also have oxidative consequences via production H(2)O(2) or sulphitolysis. To investigate effects sulphur dioxide (SO(2) ) fresh table grapes (Vitis vinifera L. 'Crimson Seedless'), transcriptome analysis was carried out...
Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as model system retrograde or stress between mitochondria nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified negative regulator (regulator alternative oxidase1a 7 [rao7] mutant) in genetic screen Arabidopsis (Arabidopsis thaliana). rao7/myb29 mutants have increased...
The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 homozygous (letm1(−/−) LETM2(+/−)) displayed a mild retarded growth phenotype during early seedling growth. It was shown accumulation proteins reduced in plants. Examination respiratory chain by Western blotting, blue native...
Protein homeostasis in eukaryotic organelles and their progenitor prokaryotes is regulated by a series of proteases including the caseinolytic protease (CLPP). CLPP has essential roles chloroplast biogenesis maintenance, but significance plant mitochondrial remains unknown factors that aid coordination nuclear- mitochondrial-encoded subunits for complex assembly mitochondria await discovery. We generated knockout lines single gene CLP subunit, CLPP2, Arabidopsis (Arabidopsis thaliana)....
Endogenous indole‐3‐acetic acid, abscisic acid and cytokinins (zeatin, zeatin riboside, N‐isopentenyladenine N‐isopentenyladenosine) were evaluated in initial explants (leaves) of vitro propagated plants alfalfa ( Medicago falcata L.) lines varying embryogenic capacity during the somatic embryogenesis process. Fast embryo‐genic induction was correlated with high IAA low ABA levels explants. No significant differences observed cytokinin contents. Our results suggest that a certain hormone...
Isoprinosine is a synthetic purine derivative with immunomodulatory and antiviral properties, which result from an apparent in vivo enhancement of host immune responses. To evaluate the serum levels certain cytokines during after isoprinosine treatment, we assigned 10 healthy volunteers to receive 1 g, 3 times daily, 5 consecutive days weekly. Both treatment follow-up phase last weeks. Interferon-gamma (IFN-gamma), interleukin-2 (IL-2), IL-10, tumor necrosis factor-alpha (TNF-alpha) were...
In Arabidopsis (Arabidopsis thaliana), small gene families encode multiple isoforms for many of the components mitochondrial protein import apparatus. There are three TRANSLOCASE OF THE INNER MEMBRANE17 (Tim17). Transcriptome analysis indicates that AtTim17-1 is only detectable in dry seed. this study, two independent transfer DNA insertional mutant lines tim17-1 exhibited a germination-specific phenotype, showing significant increase rate germination. Microarray analyses revealed Attim17-1...
A variety of eukaryotes, in particular plants, do not contain the required number tRNAs to support translation mitochondria-encoded genes and thus need import from cytosol. This study identified two Arabidopsis (Arabidopsis thaliana) proteins, Tric1 Tric2 (for tRNA component), which on simultaneous inactivation by T-DNA insertion lines displayed a severely delayed chlorotic growth phenotype significantly reduced capacity into isolated mitochondria. The predicted tRNA-binding domain Tric2,...
Complex I biogenesis requires the expression of both nuclear and mitochondrial genes, import proteins, cofactor biosynthesis, assembly at least 49 individual subunits. Assembly factors interact with subunits but are not part final holocomplex. We show that in Arabidopsis (Arabidopsis thaliana), a matrix protein (EMB1793, At1g76060), which we term COMPLEX ASSEMBLY FACTOR 1 (CIAF1), contains LYR domain is required for assembly. T-DNA insertion mutants CIAF1 lack Supercomplex I+III. Biochemical...
Evidence is presented for the role of a mitochondrial ribosomal (mitoribosomal) L18 protein in cell division, differentiation, and seed development after characterization recessive mutant, heart stopper (hes). The hes mutant produced uncellularized endosperm embryos arrested at late globular stage. differentiated partially on rescue medium with some forming callus. HES (At1g08845) encodes mitochondrially targeted member highly diverged family. substitution conserved amino residue potentially...