A5039965367- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Molecular Biology Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Genomics and Chromatin Dynamics
- Pregnancy and preeclampsia studies
- Cassava research and cyanide
- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Plant and fungal interactions
- Cancer Genomics and Diagnostics
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Developmental Biology and Gene Regulation
- Microbial Community Ecology and Physiology
- Genetic factors in colorectal cancer
- Neuroscience and Neuropharmacology Research
- Corneal Surgery and Treatments
- Protein Degradation and Inhibitors
- Chromosomal and Genetic Variations
- Congenital heart defects research
University of Otago
2013-2023
University of California, Irvine
2020-2022
Massey University
2008
University of Bath
1998-2001
City Of Hope National Medical Center
1965
United States Department of State
1965
Eastman Dental Hospital
1963-1964
Summary Beneficial associations between plants and microbes play an important role in both natural agricultural ecosystems. For example, fungi of the genus Epichloë , cool‐season grasses are known for their ability to increase resistance insect pests, fungal pathogens drought. However, little is about molecular changes induced by endophyte infection. To study impact infection, we compared expression profiles, based on RNA sequencing, perennial ryegrass infected with festucae noninfected...
During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds mitosis without cytokinesis. This stage development important in determining final size a model for studying aspects cellular molecular biology, such as cell cycle genomic imprinting. However, small makes high-throughput analysis technically difficult. Laser capture microdissection enabled high-resolution transcript at 4 d after pollination....
A recently emerged plant disease, bacterial canker of kiwifruit (Actinidia deliciosa and A. chinensis), is caused by Pseudomonas syringae pv. actinidiae (PSA). The disease was first reported in China Japan the 1980s. severe outbreak PSA began Italy 2008 has spread to other European countries. found both New Zealand Chile 2010. To study evolution pathogen analyse transmission between countries, genomes strains from (where genus Actinidia endemic), Italy, were sequenced. are very similar....
Abstract Background Arabidopsis thaliana is a useful model organism for deciphering the genetic determinants of seed size; however small size its seeds makes measurements difficult. Bulk weights are often used as an indicator average size, but details individual obscured. Analysis images possible issues arise from variations in pigmentation and shadowing making analysis laborious. We therefore investigated use consumer level scanner to facilitate conjunction with open source image-processing...
Abstract The germline is the only cellular lineage capable of transferring genetic information from one generation to next. Intergenerational transmission epigenetic memory through germline, in form DNA methylation, has been proposed; however, mammals this largely prevented by extensive erasure during definition. Here we report that, unlike mammals, continuously-defined ‘preformed’ zebrafish does not undergo genome-wide methylation development. Our analysis also uncovers oocyte-specific...
Aquatic environmental DNA (eDNA) surveys are transforming how marine ecosystems monitored. The time-consuming preprocessing step of active filtration, however, remains a bottleneck. Hence, new approaches that eliminate the need for filtration required. Filter-feeding invertebrates have been proven to collect eDNA, but side-by-side comparative studies investigate similarity between aquatic and filter-feeder eDNA signals essential. Here, we investigated differences among four sources (water;...
Control of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but innate mechanisms are unknown. The aim was to study phenylpropanoids and associated enzymes as possible defence components.
Increased resilience of pasture grasses mediated by fungal Epichloë endophytes is crucial to pastoral industries. The underlying mechanisms are only partially understood and likely involve very different activities the endophyte in plant tissues responses these. We analyzed transcriptomes festucae its host, Lolium perenne, host function developmental stages. contributed approximately 10× more than biomass infected tissues. Proliferating mycelium growing highly expressed genes involved hyphal...
Abstract Background Epimorphic regeneration is the process by which complete of a complex structure such as limb occurs through production proliferating blastema. This type rare among vertebrates but does occur in African clawed frog Xenopus laevis , traditionally model organism for study early development. tadpoles can regenerate their tails, buds and lens eye, although ability latter two organs to diminishes with advancing developmental stage. Using heat shock inducible transgene that...
PurposeA few de novo missense variants in the cytoplasmic FMRP-interacting protein 2 (CYFIP2) gene have recently been described as a novel cause of severe intellectual disability, seizures, and hypotonia 18 individuals, with p.Arg87 substitutions majority.MethodsWe assembled data from 19 newly identified all previously published individuals CYFIP2 variants. By structural modeling investigation WAVE-regulatory complex (WRC)-mediated actin polymerization six patient fibroblast lines we...
Abstract Environmental DNA (eDNA) metabarcoding has shown great promise as an effective, non‐invasive monitoring method for marine biomes. However, long filtration times and the need state‐of‐the‐art laboratories are restricting sample replication in situ species detections. Methodological innovations, such passive self‐contained extraction protocols, have potential to alleviate these issues. We explored implementation of sampling a protocol by comparing fish diversity obtained from active...
Surgical removal of the lens from larval Xenopus laevis results in a rapid transdifferention central corneal cells to form new lens. The trigger for this process is understood be an induction event arising unprecedented exposure cornea vitreous humour that occurs following removal. molecular identity unknown.Here, we have used functional transgenic approach show BMP signalling required regeneration and microarray identify genes are upregulated specifically during process. Analysis array data...
New Zealand Māori have a considerably higher incidence of gastric cancer compared to non-Māori, and are one the few populations worldwide with prevalence diffuse-type disease. Pathogenic germline CDH1 mutations causative hereditary diffuse cancer, predisposition syndrome primarily characterised by an extreme lifetime risk developing cancer. well described in families Zealand. However, contribution these high is unknown. We used next-generation sequencing, Sanger Multiplex Ligation-dependent...
The mature cerebral cortex contains a wide diversity of neuron phenotypes. This is specified during development by neuron-specific expression key transcription factors, some which are retained for the life animal. One these developmental factors that also in adult Fezf2, but types expressing it unknown. With validated Fezf2-Gfp reporter mouse, whole-cell electrophysiology with morphology reconstruction, cluster analysis, vivo retrograde labeling, and immunohistochemistry, we identify...
To compare the Roche Cell-Free DNA Collection Tubes® against Streck BCT®s for sample stability using Cell Free (cfDNA) from healthy volunteers (n = 20).Whole blood was drawn into five and tubes per volunteer, stored at room temperature processed different time points (Days 0, 4, 7, 10 14). One volunteer had ×10 K3EDTA to observe effect of no preservation buffer on White Blood (WBC) lysis. extracted plasma concentration (ng/μL) measured Qubit Fluorometer® each point. The eluted further...
While overall colorectal cancer (CRC) cases have been declining worldwide there has an increase in the incidence of disease among patients under 50 years age. Mutation APC gene is a common early event CRC but reported at lower rates early-onset (EOCRC) than older patients. Here we investigate mutation status cohort EOCRC New Zealand using novel sequencing approach targeting regions encompassing vast majority known mutations. Using this strategy find higher rate (72%) previously with...
Laser microdissection (LM) of biological specimens is enabling high‐throughput molecular analysis to be carried out with a cellular or tissue‐specific resolution. If particular structure identifiable using conventional light microscope then it most likely available for isolation by LM, this includes subcellular structures such as organelles even individual chromosomes. To obtain reliable data from laser microdissected samples several protocols need established and in many cases optimized...
Laser microdissection (LM) provides a useful method for isolating specific cells or tissues from biological samples. Here, we adapted protocols to allow high-resolution transcript analysis of different developing Arabidopsis seed. Sufficient RNA (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mo>∼</mml:mo></mml:math>50 ng) was extracted endosperm tissue RT-PCR. However, obtain enough microarray analyses, it necessary amplify the RNA. PCR- and IVT-based amplification methods...