A5064798533- Glioma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Heat shock proteins research
- Extracellular vesicles in disease
- Microbial Metabolism and Applications
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Biochemical and biochemical processes
- Protein Degradation and Inhibitors
- Macrophage Migration Inhibitory Factor
- Radiomics and Machine Learning in Medical Imaging
- Corporate Governance and Law
- Nuclear Receptors and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Enzyme-mediated dye degradation
- DNA Repair Mechanisms
- RNA Research and Splicing
German Cancer Research Center
2022-2025
Heidelberg University
2022-2025
University Medical Centre Mannheim
2025
University Hospital Heidelberg
2025
Max Planck Institute for Multidisciplinary Sciences
2024
Tissue Dynamics (Israel)
2024
Hopp Children's Cancer Center Heidelberg
2023
Universitätsmedizin Göttingen
2021
Heinrich Heine University Düsseldorf
2014
Macrophage migration inhibitory factor (MIF) is an upstream regulator of innate immunity, but its expression increased in some cancers via stabilization with HSP90-associated chaperones. Here, we show that MIF tumor-specific acute colitis-associated colorectal cancer (CRC) mouse model, leading to functions and selective therapeutic vulnerabilities. Therefore, demonstrate a Mif deletion reduced CRC tumor growth. Further, define dual role for progression. protects mice from...
Abstract Pediatric low-grade gliomas (pLGG) show heterogeneous responses to MAPK inhibitors (MAPKi) in clinical trials. Thus, more complex stratification biomarkers are needed identify patients likely benefit from MAPKi therapy. Here, we MAPK-related genes enriched MAPKi-sensitive cell lines using the GDSC dataset and apply them calculate class-specific sensitivity scores (MSSs) via single-sample gene set enrichment analysis. The MSSs discriminate non-sensitive cells significantly correlate...
Missense p53 mutations (mutp53) occur in approx. 70% of pancreatic ductal adenocarcinomas (PDAC). Typically, mutp53 proteins are aberrantly stabilized by Hsp90/Hsp70/Hsp40 chaperone complexes. Notably, stabilization is a precondition for specific alleles to acquire powerful neomorphic oncogenic gain-of-functions (GOFs) that promote tumor progression solid cancers mainly increasing invasion and metastasis. In colorectal cancer (CRC), we recently established the common hotspot mutants...
Summary Dishevelled (Dvl) proteins are essential transducers in Wnt signaling pathways, which have been implicated development, stem cell maintenance, and human diseases such as cancer. Several studies shown that Dvl form dynamic biomolecular condensates. However, how cellular signals states influence the formation of condensates remains poorly understood. Here, we analyzed cells with endogenous Dvl2 using image-based sorting combination phosphoproteomics identified protein enrichment for...
WNT signaling governs development, homeostasis, and aging of cells tissues, is frequently dysregulated in pathophysiological processes such as cancer. proteins are hydrophobic traverse the intercellular space between secreting receiving on various carriers, including extracellular vesicles (EVs). Here, we address relevance different EV fractions other vehicles for WNT5a protein, a non‐canonical ligand that signals independently beta‐catenin. Its highly context‐dependent roles cancer (either...
Abstract Wnt signaling controls cell proliferation, differentiation, and migration, with critical roles in diseases such as cancer. It was proposed that relies on the formation of dynamic biomolecular condensates, “signalosome” “destruction complex”, a potential to modify spatial temporal regulation outcomes. To investigate role phase separation signaling, we generated line endogenously tagged Dvl2, central scaffolding protein, using mEos3.2 fluorophore. Applying live-cell single-molecule...
Melanoma is the most serious and deadly form of skin cancer with progression to advanced melanoma, intrinsically disordered protein α-synuclein upregulated high levels. While toxic dopaminergic neurons in Parkinson’s disease, highly beneficial for primary metastatic melanoma cells. To gain detailed insights into this exact opposite role we performed proteomic studies high-level α-synuclein-expressing human cell lines that were treated diphenyl-pyrazole small-molecule compound anle138b, which...
The vast majority of human tumors with p53 mutations undergo loss the remaining wildtype allele (loss-of-heterozygosity, p53LOH). p53LOH has watershed significance in promoting tumor progression. However, driving forces for are poorly understood. Here we identify repressive WTp53-HSF1 axis as one driver p53LOH. We find that WTp53 AOM/DSS chemically-induced colorectal (CRC) p53R248Q/+ mice retains partial activity and represses heat-shock factor 1 (HSF1), master regulator proteotoxic stress...
Abstract Extracellular vesicles (EVs) are small, membrane-bound structures released by cells. They contain various biomolecules and have emerged as mediators of cellular signaling. EVs origins can be isolated from body fluids hold potential novel biomarkers. However, their clinical implementation biomarkers is hampered complex isolation procedures, limited expertise in routine labs, a lack mechanistic understanding signal transfer, particularly vivo. To better understand how carry oncogenic...
Abstract INTRODUCTION Pediatric low-grade gliomas (pLGG), the most common brain tumors in children, are driven by alterations MAPK pathway. Several clinical trials have shown potential for inhibitors (MAPKi) treatment pLGG. However, range of response is broad, even within entities sharing same driving genetic alteration. A predictive stratification tool needed to identify patients that will be more likely benefit from MAPKi therapy. METHODS We generated gene-expression-based sensitivity...
Abstract Pediatric low-grade glioma (pLGG), the most common brain tumors in children, are driven by alterations MAPK pathway. Several clinical trials have shown potential for inhibitor (MAPKi) treatment pLGG. However, range of response to MAPKi is heterogeneous, even between sharing same driving alteration. A predictive stratification tool needed identify that will be sensitive inhibition. We generated sensitivity gene signatures each class (BRAFi, MEKi, ERKi), based on MAPK-related genes...