A5012280023- Glioma Diagnosis and Treatment
- Neurofibromatosis and Schwannoma Cases
- Meningioma and schwannoma management
- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Cancer-related molecular mechanisms research
- Respiratory Support and Mechanisms
- MicroRNA in disease regulation
- Traumatic Brain Injury and Neurovascular Disturbances
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Hepatitis C virus research
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Hereditary Neurological Disorders
- Systemic Lupus Erythematosus Research
- Genomic variations and chromosomal abnormalities
- Cardiac Valve Diseases and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Amoebic Infections and Treatments
- Autoimmune Neurological Disorders and Treatments
Humboldt-Universität zu Berlin
2019-2024
Charité - Universitätsmedizin Berlin
2014-2024
Freie Universität Berlin
2019-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2021
Klinikum im Friedrichshain
2017-2019
Jena University Hospital
2003-2017
Friedrich Schiller University Jena
2017
Centre Hospitalier Universitaire de Lille
1996
Radboud University Nijmegen
1986
Abstract Introduction A hallmark of pediatric low-grade glioma (pLGG) is aberrant signaling the mitogen activated protein kinase (MAPK) pathway. Hence, inhibition MAPK using small molecule inhibitors such as MEK (MEKi) may be a promising strategy. Methods In this multi-center retrospective centrally reviewed study, we analyzed 18 patients treated with MEKi trametinib for progressive pLGG an individual treatment decision between 2015 and 2019. We have investigated radiological response per...
Abstract Pediatric low-grade gliomas (pLGG) show heterogeneous responses to MAPK inhibitors (MAPKi) in clinical trials. Thus, more complex stratification biomarkers are needed identify patients likely benefit from MAPKi therapy. Here, we MAPK-related genes enriched MAPKi-sensitive cell lines using the GDSC dataset and apply them calculate class-specific sensitivity scores (MSSs) via single-sample gene set enrichment analysis. The MSSs discriminate non-sensitive cells significantly correlate...
Abstract Background The international, multicenter registry LOGGIC Core BioClinical Data Bank aims to enhance the understanding of tumor biology in pediatric low-grade glioma (pLGG) and provide clinical molecular data support treatment decisions interventional trial participation. Hence, question arises whether implementation RNA sequencing (RNA-Seq) using fresh frozen (FrFr) tissue addition gene panel DNA methylation analysis improves diagnostic accuracy provides additional benefit. Methods...
Thrombin, the ligand of protease-activated receptor 1 (PAR1), is a well-known stimulator proangiogenic responses in vascular endothelial cells (ECs), which are mediated through induction growth factor (VEGF). However, transcriptional events underlying this thrombin-induced VEGF and angiogenic response less well understood at present. As reported here, we conducted detailed promotor activation signal transduction pathway studies human microvascular ECs, to decipher transcription factors...
Abstract Background Although cavitating ultrasonic aspirators are commonly used in neurosurgical procedures, the suitability of aspirator-derived tumor material for diagnostic procedures is still controversial. Here, we explore feasibility using aspirator-resected tissue to classify otherwise discarded sample by fast DNA methylation-based analysis low pass nanopore whole genome sequencing. Methods Ultrasonic specimens from pediatric patients undergoing brain resection were subjected low-pass...
NANOG, as a key regulator of pluripotency and acting synergistically with other factors, has been described crucial transcription factor in various types cancer. In meningiomas the expression this marker not yet described. With our study, we aimed to identify localize NANOG possible markers pluripotency, stem cell properties differentiation meningioma tissue, elucidate effect on tumorigenesis. The gene levels ( NANOG1 NANOGP8 ), SOX2 , OCT4 KLF4 ABCG2 CMYC MSI1 CD44 NOTCH1 NES SALL4B TP53...
Scleroderma renal crisis (SRC) is a life-threatening complication of systemic sclerosis (SSc). Autoantibodies (Abs) against endothelial cell antigens have been implicated in SSc and SRC. However, their detailed roles remain poorly defined. Pro-inflammatory cytokine interleukin-6 (IL-6) has found to be increased SSc, but its role SRC unclear. Here, we aimed determine how the autoantibodies from patients with affect IL-6 secretion by micro-vascular cells (HMECs). Serum IgG fractions were...
Neurosurgical treatment is an integral part of the algorithms for pediatric low-grade glioma (LGG), yet patterns surgical procedures are rarely challenged. The objective this study was to evaluate in LGG.
Abstract Background Intracerebral schwannomas are rare tumors resembling their peripheral nerve sheath counterparts but localized in the CNS. They not classified as a separate tumor type 2021 WHO classification. This study aimed to compile and characterize these neoplasms morphologically molecularly. Methods We analyzed 20 samples by histology, RNA Next-Generation Sequencing, DNA-methylation profiling, copy number analyses, single nucleus sequencing (snRNA-seq). Clinical data, including age,...
Optic pathway gliomas are often asymptomatic tumors occurring in children with neurofibromatosis type 1 (NF1 + OPG) or sporadically (spOPG). Treatment is usually prompted by visual loss and/or tumor progression on MRI. The aim of this study was to investigate the relationship between acuity (VA), growth, and contrast enhancement provide more distinct indications for administration gadolinium-based agents.Tumor load retrospectively measured semi-quantitatively scored 298 MRIs 35 patients (63%...
Abstract Surgical resection is a mainstay of treatment for pediatric low‐grade glioma (LGG) within all current therapy algorithms, yet associated morbidity scarcely reported. As supratentorial midline (SML) interventions are particularly challenging, we investigated the frequency neurosurgical complications/new impairments aiming to identify their risk factors. Records were retrospectively analyzed from 318 patients with SML‐LGG successive German multicenter LGG studies, undergoing surgery...
To compare endtidal and transcutaneous respiratory monitoring of high-frequency jet ventilation (HFJV) in rigid bronchoscopy. Both techniques provide a noninvasive measurement pCO2.High-frequency was applied via bronchoscope. Driving pressure (DP) initially adapted to ensure normal ventilation. It then changed twice by +/- 30% from the initial value. Endtidal data were compared with arterial blood gas (ABG).Results analyzed separately for time just after changing driving (10 min) remaining...
KIAA1549-BRAF fusions occur in certain brain tumours and provide druggable targets due to a constitutive activation of the MAP-kinase pathway. We introduce workflows for calling fusion from DNA methylation array-derived copy number as well panel sequencing data.Copy profiles were analysed by automated screening visual verification tandem duplication on chromosome 7q34, indicative fusion. Pilocytic astrocytomas ICGC cohort with known status used validation. called data using callers Manta,...
In this study the effects of nicomorphine, administered either intramuscularly or by high thoracic epidural route, on ventilatory and airway occlusion pressure response to CO 2 were investigated compared. Twenty‐four patients scheduled for surgery allocated randomly postoperative pain relief i.m. nicomorphine nicomorphine. The 5% carbon dioxide was measured in all patients: first 1 day before operation, secondly after immediately administration finally administration, at moment when no...
Abstract Aims DNA methylation profiling, recently endorsed by the World Health Organisation (WHO) as a pivotal diagnostic tool for brain tumours, most commonly relies on bead arrays. Despite its widespread use, limited data exist technical reproducibility and potential cross‐institutional differences. The LOGGIC Core BioClinical Data Bank registry conducted prospective laboratory comparison trial with 12 international laboratories to enhance accuracy paediatric low‐grade gliomas, focusing...
Abstract Background Although cavitating ultrasonic aspirators are commonly used in neurosurgical procedures, the suitability of aspirator-derived tumor material for diagnostic procedures is still controversial. Here, we explore feasibility using aspirator-resected tissue to classify otherwise discarded sample by fast DNA methylation-based analysis low pass nanopore whole genome sequencing. Methods specimens from pediatric patients undergoing brain tumour resection were subjected low-pass...
The assessment of the skin flap above cranial defects (SCD) following craniectomy is routine in neurosurgical practice, and a change consistency may indicate raised intracranial pressure or occurrence complication necessitating intervention. purpose this study was to develop clinically useful classification system based on clinical degree bulging sinking its firmness. This prospective single-center study. SCDs consecutive patients who underwent were assessed daily by two trained independent...