A5008381398- Plant Pathogens and Fungal Diseases
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- DNA and Biological Computing
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Cancer-related gene regulation
Inserm
2017-2024
Aix-Marseille Université
2017-2021
Theories and Approaches of Genomic Complexity
2020-2021
Université Paris-Saclay
2021
CEA Paris-Saclay
2021
Institut de Biologie Intégrative de la Cellule
2017-2021
Centre National de la Recherche Scientifique
2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021
Abstract Gene expression is controlled by the involvement of gene-proximal (promoters) and distal (enhancers) regulatory elements. Our previous results demonstrated that a subset gene promoters, termed Epromoters, work as bona fide enhancers regulate expression. Here, we hypothesized Epromoters play key role in coordination rapid induction during inflammatory response. Using high-throughput reporter assay explored function response to type I interferon. We find clusters IFNa-induced genes...
Abstract Background Selective gene silencing is key to development. It generally accepted that H3K27me3-enriched heterochromatin maintains transcriptional repression established during early development and regulates cell fate. Conversely, H3K9me3-enriched prevents differentiation but constitutes protection against transposable elements. We exploited the fungus Podospora anserina , a valuable alternative higher eukaryote models, question biological relevance functional interplay of these two...
Mapping the binding sites of DNA- or chromatin-interacting proteins is essential to understanding biological processes. DNA adenine methyltransferase identification (DamID) has emerged as a comprehensive method map genome-wide occupancy interest. A caveat DamID specificity Dam for GATC motifs that are not homogenously distributed in genome. Here, we developed an optimized named MadID, using proximity labeling by M.EcoGII. M.EcoGII mediates N6-adenosine methylation any sequence context,...
Stress insults trigger the rapid and global reprogramming of gene transcription by coordinated recruitment a limited number key inducible factors to cis-regulatory elements. Here, we performed comprehensive analysis different stress models observed that co-induced genes are generally located in close genomic proximity. By integrating expression factor binding resources models, found an enrichment for clusters whereby only one promoters cluster recruits factors, reminiscent Epromoters, type...
Abstract Selective gene silencing is key to development. The H3K27me3 enriched heterochromatin maintains transcription repression established during early development and regulates cell fate. Conversely, H3K9me3 prevents differentiation but constitutes a permanent protection against transposable element. We exploited the fungus Podospora anserina , valuable alternative higher eukaryote models question biological relevance interplay of these two distinct conformations. found that...